Results 51 to 60 of about 939 (180)

Intestinal Organoids in Colitis Research: Focusing on Variability and Cryopreservation [PDF]

, 2021
In recent years, stem cell-derived organoids have become a cell culture standard that is widely used for studying various scientific issues that were previously investigated through animal experiments and using common tumor cell lines.
Bauer, Verena N., Bleich, André, Buettner, Manuela, Czech, Sandra, Dittrich-Breiholz, Oliver, Donath, Sören, Kalies, Stefan, Kosanke, Maike, Liese, Andrea, zur Bruegge, Talke F.   +9 more
core   +2 more sources

Colitis-induced upregulation of tumor necrosis factor receptor-2 (TNFR2) terminates epithelial regenerative signaling to restore homeostasis

iScience, 2023
Summary: Colonic epithelial repair is a key determinant of health. Repair involves changes in epithelial differentiation, an extensive proliferative response, and upregulation of regeneration-associated “fetal-like” transcripts, including Ly6a (Sca-1 ...
Zohreh Sharifkhodaei, Cambrian Y. Liu, Nandini Girish, Ying Huang, Shivesh Punit, M. Kay Washington, D. Brent Polk   +6 more
doaj   +1 more source

Opinion: Are Organoids the End of Model Evolution for Studying Host Intestinal Epithelium/Microbe Interactions?

Microorganisms, 2019
In the pursuit to understand intestinal epithelial cell biology in health and disease, researchers have established various model systems, from whole animals (typically rodents) with experimentally induced disease to transformed human carcinomas.
Michelle M. George, Mushfiqur Rahman, Jessica Connors, Andrew W. Stadnyk   +3 more
doaj   +1 more source

Human Enteroids/Colonoids and Intestinal Organoids Functionally Recapitulate Normal Intestinal Physiology and Pathophysiology [PDF]

Journal of Biological Chemistry, 2016
Identification of Lgr5 as the intestinal stem cell marker as well as the growth factors necessary to replicate adult intestinal stem cell division has led to the establishment of the methods to generate "indefinite" ex vivo primary intestinal epithelial cultures, termed "mini-intestines." Primary cultures developed from isolated intestinal crypts or ...
Zachos, NC, Kovbasnjuk, O, Foulke-Abel, J, In, J, Blutt, SE, de Jonge, Hugo, Estes, MK, Donowitz, M   +7 more
openaire   +2 more sources

An in vitro chronic damage model impairs inflammatory and regenerative responses in human colonoid monolayers [PDF]

Cell Reports, 2022
Acute damage to the intestinal epithelium can be repaired via de-differentiation of mature intestinal epithelial cells (IECs) to a stem-like state, but there is a lack of knowledge on how intestinal stem cells function after chronic injury, such as in inflammatory bowel disease (IBD).
William D. Rees, Nikita Telkar, David T.S. Lin, May Q. Wong, Chad Poloni, Ayda Fathi, Michael Kobor, Nicholas C. Zachos, Theodore S. Steiner   +8 more
openaire   +2 more sources

Heat-inactivated Bifidobacterium adolescentis ameliorates colon senescence through Paneth-like-cell-mediated stem cell activation

Nature Communications, 2023
Declined numbers and weakened functions of intestinal stem cells (ISCs) impair the integrity of the intestinal epithelium during aging. However, the impact of intestinal microbiota on ISCs in this process is unclear.
Yadong Qi, Jiamin He, Yawen Zhang, Qiwei Ge, Qiwen Wang, Luyi Chen, Jilei Xu, Lan Wang, Xueqin Chen, Dingjiacheng Jia, Yifeng Lin, Chaochao Xu, Ying Zhang, Tongyao Hou, Jianmin Si, Shujie Chen, Liangjing Wang   +16 more
doaj   +1 more source

Long-term culture of intestinal cell progenitors: an overview of their development, application, and associated technologies [PDF]

, 2016
Purpose of Review Long-term culture of adult progenitor cells in 3D is a recently emerging technology that inhabits the space between 2D cell lines and organ slice culture.
Hollins, Andrew John, Parry, Lee
core   +1 more source

Human iPSC Colonoid Function is Improved by Exposure to Fecal Fermentates

The FASEB Journal, 2021
The host‐microbe interaction is critical for intestinal homeostasis. By‐products from microbial metabolism of unabsorbed dietary components have been studied increasingly as potential contributors to health and disease. In vitro fermentation systems provide a way to simulate microbial activity and by‐product production of the colon using human fecal ...
Sarah Pearce, Gregory Weber, Laurel Doherty, Jason Soares   +3 more
openaire   +1 more source

A8 REPEATED SUBMERGENCE OF AIR-LIQUID INTERFACE COLONOID CULTURES IMPAIRS INFLAMMATORY AND REGENERATIVE RESPONSES [PDF]

Journal of the Canadian Association of Gastroenterology, 2022
Abstract Background Damage in the intestinal epithelium is repaired via de-differentiation of mature intestinal epithelial cells to a stem-like state. Indeed, literature has primarily focused on acute forms of intestinal damage, but there is a lack of models to study how intestinal stem cells function
W D Rees, N Telkar, D Lin, M Wong, C Poloni, A Fathi, M Kobor, N Zachos, S Ted   +8 more
openaire   +1 more source

Loss of SLC26A3 Results in Colonic Mucosal Immune Dysregulation via Epithelial-Immune Cell Crosstalk

Cellular and Molecular Gastroenterology and Hepatology, 2023
Background & Aims: Down-regulation of chloride transporter SLC26A3 or down-regulated in adenoma (DRA) in colonocytes has recently been linked to the pathogenesis of ulcerative colitis (UC).
Dulari Jayawardena, Shubha Priyamvada, Takahiro Kageyama, Zachary White, Anoop Kumar, Theodor F. Griggs, Apurba Majumder, Ramsha Akram, Arivarasu N. Anbazhagan, Teruyuki Sano, Pradeep K. Dudeja   +10 more
doaj   +1 more source