Results 91 to 100 of about 33,064 (290)

The next three decades of the comet assay: a report of the 11th International Comet Assay Workshop

, 2017
The International Comet Assay Workshops are a series of scientifc conferences dealing with practical and theoretical aspects of the Comet Assay (single-cell gel electrophoresis)-a simple method for detecting DNA strand breaks.
Sabine A. S. Langie, Langie, Sabine A.S.; id_orcid, Koppen, Gudrun, Collins, Andrew R., Gudrun Koppen, Gunnar Brunborg, Azqueta, Amaya, Brunborg, Gunnar, Pourrut, Bertrand, Andrew R. Collins, Bertrand Pourrut, Amaya Azqueta   +11 more
core   +1 more source

Visualizing Specific Tubulin Isotypes and Pathogenic Variants in Cellular Microtubule Arrays

Cytoskeleton, EarlyView.
ABSTRACT Eukaryotic cells depend on dynamic microtubule arrays to execute a wide range of functions vital for life. These microtubule filaments are formed through the polymerization of α/β‐tubulin proteins, which can be generated from numerous tubulin genes, or isotypes.
Sareen Fiaz, Benjamin Powers, Jayne Aiken   +2 more
wiley   +1 more source

Genomic instability in patients with type 2 diabetes mellitus on hemodialysis

Revista Brasileira de Hematologia e Hemoterapia, 2012
OBJECTIVE: A previous study by our research group evaluated the levels of DNA damage using the comet assay in hemodialysis patients with type 2 diabetes mellitus. The same blood samples were also evaluated using the cytochalasin B micronucleus assay.
Roberta Passos Palazzo, Pamela Brambilla Bagatini, Patrícia Brandt Schefer, Fabiana Michelsen de Andrade, Sharbel Weidner Maluf   +4 more
doaj   +1 more source

Standardization of the COMET-assay

, 2022
S.S113The COMET-assay is a widespread method for determination of genetic damage on the DNA level. Mostly, it is conducted on a lower level of automation including a semi-automated Comet scoring procedure. However, with the increasing knowledge about the
Ritter, D., Knebel, J.
core   +1 more source

Impact of Methylparaben on Cumulus Cell DNA Integrity and Porcine Oocyte Developmental Competence In Vitro

Journal of Applied Toxicology, EarlyView.
ABSTRACT Parabens (PBs) are compounds widely used as preservatives in personal care products, food, and pharmaceuticals. Methylparaben (MePB) is the most used by different industries due to its chemical properties and low cost. The high daily human exposure to these compounds has raised concerns about their potential effects on health, particularly on ...
Yenny Ramírez‐Jara, Brenda Casado, Miguel Betancourt, Adyeni Barajas‐Salinas, Eduardo Casas, Gabriela Hurtado‐Alvarado, Juan José Rodríguez‐Mercado, Alma López, Iván Bahena, Lizbeth Juárez‐Rojas, Leticia González‐Núñez, Fahiel Casillas   +11 more
wiley   +1 more source

Health Biomarkers of Pesticide Exposure in Rural Populations of Santa Fe, Argentina: Oxidative Stress and Genotoxicity

Journal of Applied Toxicology, EarlyView.
ABSTRACT The present study aimed to quantify pesticide residues in environmental matrices and plasma and to evaluate their potential effects on oxidative stress and DNA damage in rural populations of Santa Fe province. Soil and rainwater samples were collected, and biological samples and semistructured surveys were obtained from volunteers in three ...
María Priscila Dechiara, Carlina Leila Colussi, Lucía Magdalena Odetti, Damián Marino, María Fernanda Simoniello   +4 more
wiley   +1 more source

Genotoxicity assessment in estuarine environments using oyster hemocytes: a comparative comet assay study

Ocean and Coastal Research
The comet assay with sentinel organisms has been employed to determine the genotoxicity of water samples for environmental risk or hazard assessment. Methodologies with diverse sensitivities have been proposed to elucidate the sources of genetic damage,
Clovis Lira da Rocha-Júnior, Luis Fernando Carvalho-Costa, Mateus Brandão Marques, Adalto Bianchini, Ricardo Luvizotto-Santos   +4 more
doaj   +1 more source

DNA damage in buccal cells in oral PMDs and malignant disorders by comet assay

Brazilian Journal of Oral Sciences, 2019
Aim: DNA damage associated with Oral Squamous Cell Carcinoma (OSCC) and potentially malignant disorders (PMDs) is produced due to carcinogenic agents or increased oxidative stress. Comet assay can assist in early detection and evaluation of the amount of
Garima Rawat, Aadithya B Urs, Anita Chakravarti, Priya Kumar   +3 more
doaj   +1 more source

Bisphenol A Induces DNA Damage and Differential Cytotoxicity in Thyroid Cells: High‐Dose Effects Challenge Regulatory Thresholds

Journal of Applied Toxicology, EarlyView.
ABSTRACT Bisphenol A (BPA), a ubiquitous environmental endocrine disruptor, has been implicated in carcinogenesis; however, its effects on thyroid cells remain unclear. This study investigated BPA‐induced cytotoxicity and genotoxicity in thyroid cell lines, including normal follicular (Nthy‐ori 3‐1), papillary carcinoma (TPC‐1 and BCPAP) and anaplastic
Izabela Fernanda Dal' Bó, Natássia Elena Bufalo, Valdemar Máximo, Laura Sterian Ward   +3 more
wiley   +1 more source

Detection of Hypoxia in Human Brain Tumor Xenografts Using a Modified Comet Assay

Neoplasia: An International Journal for Oncology Research, 2003
We used the standard comet assay successfully to generate in vitro dose-response curves under oxic and hypoxic conditions. We then made mixtures of cells that had been irradiated with 3 and 9 Gy of X-rays to simulate two subpopulations in a tumor, but ...
Jingli Wang, Jack Klem, Jan B. Wyrick, Tomoko Ozawa, Erin Cunningham, Jay Golinveaux, Max J. Allen, Kathleen R. Lamborn, Dennis F. Deen   +8 more
doaj   +1 more source