Results 301 to 310 of about 57,805 (348)
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Environmental Pollution
The paper provides the first assessment of the occurrence of hormetic dose responses using the Comet assay, a genotoxic assay. Using a priori evaluative criteria based on the Hormetic Database on peer-reviewed comet assay experimental findings, numerous examples of hormetic dose responses were obtained.
Edward J. Calabrese, Paul B. Selby
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The paper provides the first assessment of the occurrence of hormetic dose responses using the Comet assay, a genotoxic assay. Using a priori evaluative criteria based on the Hormetic Database on peer-reviewed comet assay experimental findings, numerous examples of hormetic dose responses were obtained.
Edward J. Calabrese, Paul B. Selby
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2018
The comet assay is one of the techniques that has been extensively used to assess DNA damage in both the somatic cells and the spermatozoa over the past years. The results are robust and, as difference with other strategies to assess DNA damage, information about the presence of single-strand breaks (SSBs) or double-strand breaks (DSBs) can be obtained.
Elva I. Cortés-Gutiérrez +2 more
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The comet assay is one of the techniques that has been extensively used to assess DNA damage in both the somatic cells and the spermatozoa over the past years. The results are robust and, as difference with other strategies to assess DNA damage, information about the presence of single-strand breaks (SSBs) or double-strand breaks (DSBs) can be obtained.
Elva I. Cortés-Gutiérrez +2 more
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Miniaturizing the comet assay with 3D vertical comets
Cytometry Part A, 2002AbstractBackgroundThe comet or single‐cell gel electrophoresis assay is a sensitive method for the detection of DNA damage. The main drawback of comet sampling is the low cell density necessary to prevent nucleus overlap after electrophoresis, which limits large‐scale high throughput screening. Another problem may be inconsistent comet focusing.
Philippe, Baert, Patrick, Van Oostveldt
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The Liverbeads® as a tool for the comet assay
Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2002Liverbeads, cryopreserved hepatocytes entrapped within an alginate matrix, were examined for their relevance in the comet assay. It was estimated by their capacity to activate the indirectly acting mutagens, cyclophosphamide (CP), benzo[a]pyrene (BP), dimethylbenzanthracene (DMBA) and 2-acetylaminofluorene (2-AAF), into DNA reactive metabolites.
Laurence, Vian +3 more
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The Comet Assay in Clinical Practice
Acta Oncologica, 1999The comet assay is a single-cell gel electrophoresis technique that measures DNA damage in individual cells. Since radiation produces 3-4 times more DNA damage in well-oxygenated cells compared with hypoxic cells, this assay can quantify the fraction of radiation-resistant hypoxic cells found in many solid tumours.
P L, Olive +9 more
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2023
The comet assay is a sensitive, inexpensive, fast, and easy-to-use method widely used in genotoxicity testing and biomonitoring studies. It can be applied using a few cells and on almost every cell type to assess a range of different DNA lesions, DNA repair activity and even DNA methylation levels, at the individual cell level. The principle is simple,
Costa, S, Pires, J, Teixeira-Gomes, A
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The comet assay is a sensitive, inexpensive, fast, and easy-to-use method widely used in genotoxicity testing and biomonitoring studies. It can be applied using a few cells and on almost every cell type to assess a range of different DNA lesions, DNA repair activity and even DNA methylation levels, at the individual cell level. The principle is simple,
Costa, S, Pires, J, Teixeira-Gomes, A
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The comet assay: a comprehensive review
Mutation Research/Reviews in Genetic Toxicology, 1995The comet assay is a sensitive and rapid method for DNA strand break detection in individual cells. Its use has increased significantly in the past few years. This paper is a review of the studies published to date that have made use of the comet assay.
D W, Fairbairn, P L, Olive, K L, O'Neill
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The Comet Assay in Marine Animals
2013Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows the detection of DNA single- and double-strand breaks, as well as the presence of alkali-labile sites and cross-links. Here we describe the protocols for the single-cell gel electrophoresis (Comet) assay in its alkaline (pH > 13), mild alkaline (pH ...
Giada, Frenzilli, Brett P, Lyons
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Comet assay and early apoptosis
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2001The comet assay is a single cell gel electrophoresis test currently used as a qualitative and quantitative genotoxicity test. However, some of the results from this comet assay and current knowledge on apoptosis lead us to suspect the presence of some false positive results.
P, Choucroun +4 more
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Comet assay in neonatal sepsis
The Indian Journal of Pediatrics, 2010To determine whether the DNA damage detected using the Comet assay helps in the diagnosis of neonatal sepsisSepsis was diagnosed, based on clinical findings, laboratory findings and positive culture results, in 24 of the 48 newborns who participated in the study.
Natália C, Carvalho +3 more
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