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The Comet Assay in Marine Animals
2019Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows for the detection of DNA single- and double-strand breaks, as well as the presence of alkali labile sites and cross-links. Here we describe protocols for the single-cell gel electrophoresis (Comet assay) in its alkaline (pH > 13), mild alkaline (pH ...
Guidi, Patrizia +2 more
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Acellular comet assay: a tool for assessing variables influencing the alkaline comet assay
Radiation Protection Dosimetry, 2011In this study, an acellular modification to the alkaline comet assay to further evaluate key variables within the assay that may influence the outcome of genotoxicity studies is described. This acellular comet assay can detect differences of 0.2 Gy of (60)Co gamma-ray radiation between 0 and 1 Gy and differences of 1 Gy between 0 and 8 Gy; thus, this ...
Erin K, Kennedy +4 more
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The Comet Moment as a Measure of DNA Damage in the Comet Assay
International Journal of Radiation Biology, 1995The development of rapid assays of radiation-induced DNA damage requires the definition of reliable parameters for the evaluation of dose-response relationships to compare with cellular endpoints. We have used the single-cell gel electrophoresis (SCGE) or 'comet' assay to measure DNA damage in individual cells after irradiation.
C R, Kent +3 more
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The In Vitro and In Vivo Comet Assays
2011The strategy for testing for genotoxicity covers three main areas, namely gene mutation, chromosome aberration or breakage (clastogenicity), and chromosome loss or gain (aneuploidy). The current generalized strategy consists of assays capable of detecting all of these endpoints using in vitro assays such as the Ames test for detecting gene mutations in
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Impact of the comet assay in radiobiology
Mutation Research/Reviews in Mutation Research, 2009Until the development of single cell gel electrophoresis methods in the 1980s, measurement of radiation-induced DNA strand breaks in individual cells was limited to detection of micronuclei or chromosome breaks that measured the combined effects of exposure and repair.
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Genotoxicity of Contaminants: Comet Assay
2013International ...
Devaux, Alain, Bony, Sylvie
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2009
Among the manifold advantages of the Comet assay is the ability to evaluate very limited amounts of test agents. In many cases compounds for a research study are not commercially available and the chemicals must be individually synthesised at great expense and with large amounts of time involved.
Elizabeth D. Wagner, Michael J. Plewa
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Among the manifold advantages of the Comet assay is the ability to evaluate very limited amounts of test agents. In many cases compounds for a research study are not commercially available and the chemicals must be individually synthesised at great expense and with large amounts of time involved.
Elizabeth D. Wagner, Michael J. Plewa
openaire +1 more source
PD-L1 as a biomarker of response to immune-checkpoint inhibitors
Nature Reviews Clinical Oncology, 2021Mary Beth Beasley +2 more
exaly
Microfluidic Paper-Based Analytical Devices: From Design to Applications
Chemical Reviews, 2021Eka Noviana +2 more
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