Results 61 to 70 of about 33,064 (290)

β‐Elemene Rescues Radiation‐Induced Enteritis by Orchestrating a Host‐Microbiome Circuit That Fuels Epigenetic DNA Repair

Advanced Science, EarlyView.
This study elucidates that β‐elemene promotes cellular uptake of L. gasseri‐derived lactate by enhancing the membrane translocation of MCT1 in a CD147‐dependent manner. Intracellular lactate, through the lactylation of RBBP4 at the K26 site, recruits EP300 to the promoter regions of downstream genes (POLD1/POLD3), catalyzing H3K27ac modification.
Jiancheng He, Jiapeng Bao, Shukang Deng, Weijie Zang, Haoming Yan, Zihao Zhao, Guangze Zhang, Ruiqing Liu, Junjie Chen, Yilin Hu, Wanjiang Xue   +10 more
wiley   +1 more source

Comparing the alkaline comet with 53BP1 immunocytochemistry assay: sensitivity and cost/time effectiveness

Frontiers in Genetics, 2015
Both the comet and p53-binding protein 1 (53BP1) Immunocytochemistry (ICC) assays are well known, sensitive techniques to determine DNA damage, albeit with different perspectives.
Rajan Maynard, David Parkinson
doaj   +1 more source

A New Version of Comet Assay [PDF]

Sovremennye tehnologii v medicine, 2016
The aim of the investigation was to assess the availability of ozone to induce DNA damage in individual cells when analyzing them using the Comet assay. Materials and Methods. Experimental studies were performed on whole blood leukocytes of white non-linear intact male rats (n=16) weighing 250±25 g.
I.A. Chernigina, T.G. Shcherbatyuk
openaire   +2 more sources

DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death [PDF]

, 2021
International audienceAbstract The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations.
Mirta Milić, Møller, Peter, Laffon, Blanca, Del Bo, Cristian, Katarina Volkovova, Orlow, Irene, Volkovova, Katarina, Teixeira, João Paulo, Marco Bruzzone, Goroshinskaya, Irina, Godschalk, Roger, Monica, Neri, Marcello Ceppi, Nemeth, , Zsuzsanna, Amaya Azqueta, Collins, Andrew R., Perez, Carlos L, Danieli Benedetti, Surikova, Ekaterina I, Smolkova, Bozena, Gajski, Goran, Andrade, Vanessa, Marcos, Ricard, Lebailly, Pierre, Cavallo, Delia, Nursen Basaran, Nemeth, Zsuzsanna, Pastor Benito, Susana, Ceppi, Marcello, del Bo’, Cristian, Sabine A. S. Langie, Elisa Boutet-Robinet, Del Bo', Cristian, Zsuzsanna Nemeth, Langie, Sabine A S; id_orcid, Kruszewski, Marcin, Valdiglesias, Vanessa, Anderson, Diana, Safar, Anna, Shaposhnikov, Sergey, Alena Kažimirova, Gudrun Koppen, Andrew Collins, Staruchova, Marta, Carlos L. Perez, Gutzkow, Kristine B., Bonassi, Stefano, Bozena Smolkova, Langie, Sabine, Bruzzone, Marco, Perez, Carlos L., Žegura, Bojana, Koppen, Gudrun, Kažimirova, Alena, Hernández, Alba, Collins, Andrew Richard, Azqueta, Amaya, Pourrut, Bertrand, Roger Godschalk, Spremo-Potparević, Biljana, Perdry, Hervé, Patrizia Russo, Pastor, Susana, Magdalena Barančokova, Cinzia Lucia Ursini, Basaran, Nursen, Costa, Solange, Marta Staruchova, Goroshinskaya, Irina A., Langie, Sabine A. S., Knasmueller, Siegfried, Teixeira, João, Surikova, Ekaterina, Neri, Monica, Riso, Patrizia, Malgorzata Dobrzyńska, Marcos Dauder, Ricardo, Barančokova, Magdalena, Giovannelli, Lisa, Brunborg, Gunnar, Silvia Moretti, Irina A. Goroshinskaya, Moretti, Massimo, Vanessa Valdiglesias, Susana Pastor, Surikova, Ekaterina I., for the hCOMET project, Ricard Marcos, Costa, Carla, Dusinska, Maria, Alba Hernández, Pierre Lebailly, Hernández Bonilla, Alba, Russo, Patrizia, Juliana Da Silva, Cristian Del Bo’, Hervé Perdry, Dobrzyńska, Malgorzata, João Paulo Teixeira, Boutet-Robinet, Elisa, Milić, Mirta, Lisa Giovannelli, Goran Gajski, Ekaterina I. Surikova, Pereira, Cristiana Costa, Blanca Laffon, Rojas, Emilio, Moretti, Silvia, Maria Dusinska, Delia Cavallo, Perez, Carlos, Bruzzone, , Marco, Neri Monica, Dobrzyńska, , Malgorzata, da Silva, Juliana, Moller, Peter, Lada Živković, Goroshinskaya, Irina A, Benedetti, Danieli, Živković, Lada, Ladeira, Carina, Stopper, Helga, Stefano Bonassi, Biljana Spremo-Potparević, Anna Safar, Langie, Sabine A.S., Patrizia Riso, Collins, Andrew, Ursini, Cinzia Lucia, Gunnar Brunborg, Peter Møller   +130 more
core   +1 more source

Light‐Switched Mesenchymal Stem Cells for In Situ Exosome Amplification in Craniofacial Bone Defect Reconstruction

Advanced Science, EarlyView.
Light‐switchable MSCs (MSC‐UCNPs) were constructed by intracellular incorporation of UCNPs. Upon 980 nm irradiation, UCNPs emitted localized ultraviolet light (365 nm), activating the ROS/HEXB/LAMP1 signaling pathway to suppress lysosome–multivesicular body fusion and thereby enhance exosome biogenesis. Embedded within an injectable hydrogel, MSC‐UCNPs
Tingting Wu, Yajing Liu, Shuman Wang, Xiaoming Bai, Luyun Zhang, Yuwei Liu, Zhiwen Fu, Chen Shi   +7 more
wiley   +1 more source

The International Comet Assay Workshop [PDF]

Mutagenesis, 1998
The Comet (single cell gel electrophoresis) assay primarily measures DNA strand breakage in single cells (Singh et al., 1988). Briefly, cells are suspended in low melting point agarose on a microscope slide. The slides are put in lysing buffer to allow the DNA to unwind and then in electrophoresis buffer.
D, Anderson, M J, Plewa
openaire   +2 more sources

DNA Damage among Wood Workers Assessed with the Comet Assay

, 2016
Exposure to wood dust, a human carcinogen, is common in wood-related industries, and millions of workers are occupationally exposed to wood dust worldwide. The comet assay is a rapid, simple, and sensitive method for determining DNA damage. The objective
Hopf, Nancy B., Evin Danisman Bruschweiler, Wild, Pascal, Cong Khanh Huynh, Dessislava Savova-Bianchi, Nancy B. Hopf, Brigitta Danuser, Bruschweiler, Evin D., Savova-Bianchi, Dessislava, Danuser, Brigitta, Huynh, Cong Khanh, Pascal Wild   +11 more
core   +1 more source

PhosSight: A Unified Deep Learning Framework Boosting and Accelerating Phosphoproteome Identification to Enable Biological Discoveries

Advanced Science, EarlyView.
PhosSight is a unified deep‐learning framework for phosphoproteome identification, featured by a phosphorylation‐aware detectability predictor. It improves identification sensitivity in DDA through deep re‐localization and rescoring, accelerates DIA searches by detectability‐guided spectral library pruning, and expands phosphoproteome coverage to ...
Ben Wang, Zhiyuan Cheng, Chengying She, Hongwei Zhao, Jiahui Zhang, Lin Lv, Zhihao Yan, Hongwen Zhu, Lizhuang Liu, Yan Fu, Xinpei Yi   +10 more
wiley   +1 more source

Targeting a Myeloid–Regulatory B Cell Network Reverses Immune Paralysis in Periprosthetic Joint Infections

Advanced Science, EarlyView.
Periprosthetic joint infection establishes a sophisticated immunosuppressive network between CXCR4+ PMN‐MDSCs and Bregs, inducing profound CD8+ T cell paralysis. Alendronate effectively disrupts this CXCR4+ PMN‐MDSC–Breg axis by targeting STAT3, thereby restoring local immune surveillance.
Jintao Wu, Shutao Zhang, Yumin Lin, Juyang Jiao, Zhiwei Fu, Qimin Hong, Ziyi Zhao, Xinhua Qu, Fei Su, Bing Yue   +9 more
wiley   +1 more source

Genotoxic Effects of Etoposide, Bleomycin, and Ethyl Methanesulfonate on Cultured CHO Cells: Analysis by GC-MS/MS and Comet Assay

Journal of Nucleic Acids, 2020
To evaluate methods for analysis of genotoxic effects on mammalian cell lines, we tested the effect of three common genotoxic agents on Chinese hamster ovary (CHO) cells by single-cell gel electrophoresis (comet assay) and gas chromatography-tandem mass ...
Donald H. Atha, Erdem Coskun, Onur Erdem, Alessandro Tona, Vytas Reipa, Bryant C. Nelson   +5 more
doaj   +1 more source