The basic principles of DNA damage detection by the alkaline comet assay [PDF]
Arhiv za farmacijuThe Comet assay is a highly sensitive and rapid method for detecting DNA damage at the level of individual cells. It measures single-stranded and double-stranded DNA breaks, alkali-labile, incomplete excision repair and cross-linking sites.
Živković Lada +6 more
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Genotoxicity assessment of piperitenone oxide: an in vitro and in silico evaluation [PDF]
, 2017 Piperitenone oxide, a natural flavouring agent also known as rotundifolone, has been studied for the genotoxicity assessment by an integrated in vitro and in silico experimental approach, including the bacterial reverse mutation assay, the micronucleus ...
Abete, Lorena +9 more
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HiComet: a high-throughput comet analysis tool for large-scale DNA damage assessment
BMC Bioinformatics, 2018 Background DNA damage causes aging, cancer, and other serious diseases. The comet assay can detect multiple types of DNA lesions with high sensitivity, and it has been widely applied.
Taehoon Lee +7 more
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An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses [PDF]
, 2014 The application of the Comet assay in environmental monitoring remains challenging in face of the complexity of environmental stressors, e.g., when dealing with estuarine sediments, that hampers the drawing of cause-effect relationships.
Caeiro, Sandra +8 more
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The use of comet assay to assess global DNA methylation in human biomonitoring studies.
Frontiers in Genetics, 2015 The Comet assay is a valuable tool for the detection of DNA damage in genotoxicity and human biomonitoring studies. Throughout the years, this biomarker has undergone several adaptations in their protocol in order to increase its sensitivity and the ...
Carla Costa +2 more
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Herpes simplex virus-type1 (HSV-1) impairs DNA repair in cortical neurons [PDF]
, 2016 Several findings suggest that Herpes simplex virus-1 (HSV-1) infection plays a role in the neurodegenerative processes that characterize Alzheimer's disease (AD), but the underlying mechanisms have yet to be fully elucidated.
Alessio Cardinale +12 more
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The Comet Assay in Human Biomonitoring
, 2013 Human biomonitoring studies aim to identify potential exposures to environmental, occupational, or lifestyle toxicants in human populations and are commonly used by public health decision makers to predict disease risk. The Comet assay measures changes in genomic stability and is one of the most reliable biomarkers to indicate early biological effects ...
Anderson, Diana +2 more
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Toxoplasma gondii infection can induce retinal DNA damage:an experimental study
International Journal of Ophthalmology, 2014 AIM: To detect whether Toxoplasma gondii (T. gondii) infection of mice can induce retinal DNA damage.METHODS: A total of 20 laboratory-bred male Swiss albino mice were used and divided into four groups:control group (non-infected animals); T ...
Nagwa Mostafa El-Sayed, Eman Mohamed Aly
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Fertilization capacity with rainbow trout DNA-damaged sperm and embryo developmental success [PDF]
, 2010 Mammalian spermatozoa undergo a strong selection process along the female tract to guarantee fertilization by good quality cells, but risks of fertilization with DNA-damaged spermatozoa have been reported.
Ahmadi +31 more
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Relationship between CpG and non-CpG DNA methylation in human lymphocytes assessed with comet assay [PDF]
The Ukrainian Biochemical JournalTissue-specific DNA methylation plays an important role in the regulation of many functional processes. The methylation level in single cells can be assessed using the comet assay (single-cell gel electrophoresis), a simple and cost-effective technique ...
M. Chopei +3 more
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