Results 41 to 50 of about 22,703 (255)

The effect of different methods and image analyzers on the results of the in vivo comet assay

Genes and Environment, 2018
Introduction The in vivo comet assay is a widely used genotoxicity test that can detect DNA damage in a range of organs. It is included in the Organisation for Economic Co-operation and Development Guidelines for the Testing of Chemicals.
Takahiro Kyoya, Rika Iwamoto, Yuko Shimanura, Megumi Terada, Shuichi Masuda   +4 more
doaj   +1 more source

Photocatalytic Water Splitting on the Lunar Surface: Prospects for In Situ Resource Utilization

Advanced Materials Interfaces, EarlyView.
Water has been found in craters on the moon nearby locations which are illuminated >80% of the time. Photocatalysis uses energy from sunlight to drive chemical reactions such as water splitting to produce oxygen and hydrogen. It is a scalable technology that requires lighter equipment and utilizes resources available on the moon. ABSTRACT The discovery
Ranjani Kalyan, Thomas D. Small, Gregory F. Metha, Mahmoud Adel Hamza, Thomas J. O'Dea, Yideng Shen, Cameron J. Shearer   +6 more
wiley   +1 more source

Epithelial cells as alternative human biomatrices for comet assay

Frontiers in Genetics, 2014
The comet assay is a valuable experimental tool aimed at mapping DNA damage in human cells in vivo for environmental and occupational monitoring, as well as for therapeutic purposes, such as storage prior to transplant, during tissue engineering, and in ...
Emilio eRojas, Yolanda eLorenzo, Kristiane eHuag-Berg, Bjørn eNicolaissen, Mahara eValverde   +4 more
doaj   +1 more source

The comet assay: topical issues [PDF]

Mutagenesis, 2008
The comet assay is a versatile and sensitive method for measuring single- and double-strand breaks in DNA. The mechanism of formation of comets (under neutral or alkaline conditions) is best understood by analogy with nucleoids, in which relaxation of DNA supercoiling in a structural loop of DNA by a single DNA break releases that loop to extend into a
A. R. Collins, A. A. Oscoz, G. Brunborg, I. Gaivão, GIOVANNELLI, LISA, M. Kruszewski, C. C. Smith, R. Štetina   +7 more
openaire   +4 more sources

β‐Elemene Rescues Radiation‐Induced Enteritis by Orchestrating a Host‐Microbiome Circuit That Fuels Epigenetic DNA Repair

Advanced Science, EarlyView.
This study elucidates that β‐elemene promotes cellular uptake of L. gasseri‐derived lactate by enhancing the membrane translocation of MCT1 in a CD147‐dependent manner. Intracellular lactate, through the lactylation of RBBP4 at the K26 site, recruits EP300 to the promoter regions of downstream genes (POLD1/POLD3), catalyzing H3K27ac modification.
Jiancheng He, Jiapeng Bao, Shukang Deng, Weijie Zang, Haoming Yan, Zihao Zhao, Guangze Zhang, Ruiqing Liu, Junjie Chen, Yilin Hu, Wanjiang Xue   +10 more
wiley   +1 more source

Investigation of Genotoxic Effect of Desflurane and Sevoflurane on Periferic Lymphocyte with Comet Assay Method

Haseki Tıp Bülteni, 2016
Aim: There are several clinical and cytogenetic studies that anesthetics cause choromosomal changes. We report data on the possible genotoxic properties of two inhalational anaesthetics, sevoflurane and desflurane in lymphocytes of children evaluated for
Yeşim Işıkçı, Ayşe Çiğdem Tütüncü, Pınar Kendigelen, Serpil Çakmakkaya, Fatiş Altındaş, Güner Kaya   +5 more
doaj   +1 more source

The Effect of Obstructive Sleep Apnea on DNA Damage and Oxidative Stress [PDF]

Clinical and Experimental Otorhinolaryngology, 2013
ObjectivesObstructive sleep apnea syndrome (OSAS) is associated with repeated hypoxia and re-oxygenation. This characteristic of OSAS may cause oxidative stress and DNA damage.
Il Gyu Kang, Joo Hyun Jung, Seon Tae Kim
doaj   +1 more source

Integrative Multi‐Omics Analysis Reveals a Mitochondrial–Immune Axis Associated With Neoadjuvant Chemotherapy Response in High‐Grade Serous Ovarian Cancer

Advanced Science, EarlyView.
Integrative multi‐omics analysis delineates a mitochondrial–immune axis governing neoadjuvant chemotherapy response in high‐grade serous ovarian cancer. Immune‐active tumors exhibit enhanced B‐cell infiltration and favorable sensitivity, whereas metabolically rewired tumors display oxidative phosphorylation dependency and resistance.
Wei Jiang, Yi Wang, Xiaohang Lu, Qianlan Yao, Libing Xiang, Xu Cai, Xiaoyu Tu, Shaoxian Tang, Ying Xu, Yufan Cheng, Liming Shen, Huijuan Yang   +11 more
wiley   +1 more source

PhosSight: A Unified Deep Learning Framework Boosting and Accelerating Phosphoproteome Identification to Enable Biological Discoveries

Advanced Science, EarlyView.
PhosSight is a unified deep‐learning framework for phosphoproteome identification, featured by a phosphorylation‐aware detectability predictor. It improves identification sensitivity in DDA through deep re‐localization and rescoring, accelerates DIA searches by detectability‐guided spectral library pruning, and expands phosphoproteome coverage to ...
Ben Wang, Zhiyuan Cheng, Chengying She, Hongwei Zhao, Jiahui Zhang, Lin Lv, Zhihao Yan, Hongwen Zhu, Lizhuang Liu, Yan Fu, Xinpei Yi   +10 more
wiley   +1 more source

Genotoxic Effects of Etoposide, Bleomycin, and Ethyl Methanesulfonate on Cultured CHO Cells: Analysis by GC-MS/MS and Comet Assay

Journal of Nucleic Acids, 2020
To evaluate methods for analysis of genotoxic effects on mammalian cell lines, we tested the effect of three common genotoxic agents on Chinese hamster ovary (CHO) cells by single-cell gel electrophoresis (comet assay) and gas chromatography-tandem mass ...
Donald H. Atha, Erdem Coskun, Onur Erdem, Alessandro Tona, Vytas Reipa, Bryant C. Nelson   +5 more
doaj   +1 more source