Results 51 to 60 of about 889,387 (243)

The use of comet assay to assess global DNA methylation in human biomonitoring studies.

open access: yesFrontiers in Genetics, 2015
The Comet assay is a valuable tool for the detection of DNA damage in genotoxicity and human biomonitoring studies. Throughout the years, this biomarker has undergone several adaptations in their protocol in order to increase its sensitivity and the ...
Carla Costa   +2 more
doaj   +1 more source

HiComet: a high-throughput comet analysis tool for large-scale DNA damage assessment

open access: yesBMC Bioinformatics, 2018
Background DNA damage causes aging, cancer, and other serious diseases. The comet assay can detect multiple types of DNA lesions with high sensitivity, and it has been widely applied.
Taehoon Lee   +7 more
doaj   +1 more source

Toxoplasma gondii infection can induce retinal DNA damage:an experimental study

open access: yesInternational Journal of Ophthalmology, 2014
AIM: To detect whether Toxoplasma gondii (T. gondii) infection of mice can induce retinal DNA damage.METHODS: A total of 20 laboratory-bred male Swiss albino mice were used and divided into four groups:control group (non-infected animals); T ...
Nagwa Mostafa El-Sayed, Eman Mohamed Aly
doaj   +1 more source

Comet Assay in Cancer Chemoprevention

open access: yes, 2016
The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it
Santoro, Raffaela   +4 more
openaire   +2 more sources

Relationship between CpG and non-CpG DNA methylation in human lymphocytes assessed with comet assay [PDF]

open access: yesThe Ukrainian Biochemical Journal
Tissue-specific DNA methylation plays an important role in the regulation of many functional processes. The methylation level in single cells can be assessed using the comet assay (single-cell gel electrophoresis), a simple and cost-effective technique ...
M. Chopei   +3 more
doaj   +1 more source

In vivo comet assay in rabbit corneal epithelial cells following ocular instillation with genotoxic compounds

open access: yesGenes and Environment, 2021
Background The in vivo comet assay is used to evaluate the genotoxic potential of compounds by detecting DNA strand breaks in cells isolated from animal tissue.
Haruna Tahara   +3 more
doaj   +1 more source

A COMET ASSAY FOR CANDIDA GLABRATA INFECTION AND TREATMENT BY PENICILLIUM CHRYSOGENUM - DERIVED AgNPs

open access: yesThe Iraqi Journal of Agricultural science, 2023
The colonies of C. glabrata were described, which morphology of isolated on sabouraud dextrose agar with chloramphenicol. Biosynthesis of nanoparticles is of great importance since they are highly involved in various medical and biological applications.
Teeba H. Mohammad
doaj   +1 more source

Effect of terahertz irradiation on DNA damage repair in living cells

open access: yesFEBS Open Bio, EarlyView.
We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno   +3 more
wiley   +1 more source

The comet assay – from toy to tool

open access: yesFrontiers in Genetics, 2015
The comet assay is nowadays the most common method for measuring DNA damage and repair in single cells. It is based on the microelectrophoretic study published by Ostling and Johanson (1984) and was developed by Singh and coworkers (1988) to a versatile ...
Guenter Speit
doaj   +1 more source

Laser‐Induced Graphene from Waste Almond Shells

open access: yesAdvanced Functional Materials, EarlyView.
Almond shells, an abundant agricultural by‐product, are repurposed to create a fully bioderived almond shell/chitosan composite (ASC) degradable in soil. ASC is converted into laser‐induced graphene (LIG) by laser scribing and proposed as a substrate for transient electronics.
Yulia Steksova   +9 more
wiley   +1 more source

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