MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum
STAR Protocols, 2021 Summary: Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay.
Aoli Xiong +5 more
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Quantifying engineered nanomaterial toxicity: comparison of common cytotoxicity and gene expression measurements [PDF]
, 2017 BACKGROUND: When evaluating the toxicity of engineered nanomaterials (ENMS) it is important to use multiple bioassays based on different mechanisms of action.
Atha, Donald H. +7 more
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Modification of comet-FISH technique by using temperature instead of chemical denaturation
MethodsX, 2014 Comet-FISH technique is an extension of commonly used comet assay. Its purpose is to determine whether primary DNA damage which comet assay detects occurred within a sequence of interest that is visualized by hybridization of fluorescent probe.
Marin Mladinic, Davor Zeljezic
doaj +1 more source
A novel antibacterial peptide derived from Crocodylus siamensis haemoglobin hydrolysate induces membrane permeabilization causing iron dysregulation, oxidative stress and bacterial death [PDF]
, 2017 Aims A novel antibacterial peptide from Crocodylus siamensis haemoglobin hydrolysate (CHH) was characterized for antimicrobial activity. Methods and Results CHHs were hydrolysed for 2 h (2 h-CHH), 4 h (4h-CHH), 6 h (6 h-CHH) and 8 h (8 h-CHH). The
Adje +29 more
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TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching. [PDF]
, 2015 The AAA+ family ATPase TRIP13 is a key regulator of meiotic recombination and the spindle assembly checkpoint, acting on signaling proteins of the conserved HORMA domain family.
Corbett, Kevin D +5 more
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The Comet Assay: Tails of the (Un)expected. Use of the comet assay in pharmaceutical development.
Frontiers in Genetics, 2015 In genotoxicity testing of pharmaceuticals the rodent alkaline comet assay is being increasingly used as a second in vivo assay in addition to the in vivo micronucleus assay to mitigate in vitro positive results as recommended by regulatory guidance.
Bas-jan Van Der Leede
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Evaluation of Cytotoxicity and Genotoxicity of Acacia aroma Leaf Extracts [PDF]
, 2014 Acacia aroma, native plant from San Luis, Argentina, is commonly used as antiseptic and for healing of wounds. The present study was conducted to investigate the in vitro cytotoxicity and genotoxicity of hot aqueous extract (HAE) and ethanolic extract ...
Alcaraz, María Luciana +7 more
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Closing the gap: from public concerns about health effects to science-based testing of chemicals
Frontiers in Genetics, 2015 The comet assay is a powerful technology for measuring DNA integrity in humans and other organisms at the level of individual cells. The assay is used for research and industrial testing but suffers from a lack of standardisation, which hinders its use ...
Sergey Shaposhnikov +2 more
doaj +1 more source
DNA single-strand break repair and spinocerebellar ataxia with axonal neuropathy-1 [PDF]
, 2007 DNA single-strand breaks (SSBs) are the commonest DNA lesions arising spontaneously in cells, and if not repaired may block transcription or may be converted into potentially lethal/clastogenic DNA double-strand breaks (DSBs).
Arnaudeau +82 more
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Elevated Level of DNA Damage and Impaired Repair of Oxidative DNA Damage in Patients with Recurrent Depressive Disorder [PDF]
, 2015 Background: Depressive disorder (DD), including recurrent DD (rDD), is a severe psychological disease, which affects a large percentage of the world population.
Bielecka-Kowalska, Anna +9 more
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