Results 21 to 30 of about 58,125 (347)
Detection of DNA damage by alkaline comet assay in mouse colonic mucosa
STAR Protocols, 2021 Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin.
Elisa Boutet-Robinet +4 more
doaj +1 more source
Using a medium-throughput comet assay to evaluate the global DNA methylation status of single cells
Frontiers in Genetics, 2014 The comet assay is a simple and cost effective technique, commonly used to analyse and quantify DNA damage in individual cells. The versatility of the comet assay allows introduction of various modifications to the basic technique.
Angelique eLewies +5 more
doaj +1 more source
Lead-induced DNA damage in Vicia faba root cells: Potential involvement of oxidative stress [PDF]
, 2011 Genotoxic effects of lead (0–20 µM) were investigated in whole-plant roots of Vicia faba L., grown hydroponically under controlled conditions. Lead-induced DNA damage in V.
Jean, Séverine +3 more
core +2 more sources
Frontiers in Genetics, 2014 Sperm DNA damage is associated with fertilization failure, impaired pre-and post- embryo implantation and poor pregnancy outcome. A series of methodologies to assess DNA damage in spermatozoa have been developed but most are unable to differentiate ...
Elva I eCortes-Gutierrez +5 more
doaj +1 more source
Mutagenic impact on fish of runoff events in agricultural areas in south-west France [PDF]
, 2011 When heavy rainfall follows herbicide application, the intense surface runoff causes stream water contamination. Aquatic organisms are then briefly exposed to a complex mixture of contaminants.
Gauthier, Laury +6 more
core +2 more sources
Antioxidants and the Comet assay
Mutation Research/Reviews in Mutation Research, 2009 It is widely accepted that antioxidants, either endogenous or from the diet, play a key role in preserving health. They are able to quench radical species generated in situations of oxidative stress, either triggered by pathologies or xenobiotics, and they protect the integrity of DNA from genotoxicants.
Cemeli, Eduardo +2 more
openaire +2 more sources
In Vitro Mutagenic and Genotoxic Assessment of a Mixture of the Cyanotoxins Microcystin-LR and Cylindrospermopsin [PDF]
, 2019 The co-occurrence of various cyanobacterial toxins can potentially induce toxic effects different than those observed for single cyanotoxins, as interaction phenomena cannot be discarded. Moreover, mixtures are a more probable exposure scenario. However,
Cameán Fernández, Ana María +4 more
core +1 more source
Is the Comet Assay a Sensitive Procedure for Detecting Genotoxicity?
Journal of Nucleic Acids, 2010 Although the Comet assay, a procedure for quantitating DNA damage in mammalian cells, is considered sensitive, it has never been ascertained that its sensitivity is higher than the sensitivity of other genotoxicity assays in mammalian cells. To determine
Satomi Kawaguchi +4 more
doaj +1 more source
Low doses of ochratoxin A induce micronucleus formation and delay DNA repair in human lymphocytes [PDF]
, 2014 The contamination of food commodities by fungal toxins has attracted great interest because many of these mycotoxins are responsible for different diseases, including cancer and other chronic illnesses. Ochratoxin A (OTA) is a mycotoxin naturally present
Barrón-Vivanco, Briscia S. +9 more
core +2 more sources
Molecular Cytogenetics, 2010 Comet assay and micronucleus (MN) test are widely applied in genotoxicity testing and biomonitoring. While comet assay permits to measure direct DNA-strand breaking capacity of a tested agent MN test allows estimating the induced amount of chromosome and/
Hovhannisyan Galina G
doaj +1 more source