Results 21 to 30 of about 22,703 (255)
Molecular Cytogenetics, 2010 Comet assay and micronucleus (MN) test are widely applied in genotoxicity testing and biomonitoring. While comet assay permits to measure direct DNA-strand breaking capacity of a tested agent MN test allows estimating the induced amount of chromosome and/
Hovhannisyan Galina G
doaj +1 more source
Modification of comet-FISH technique by using temperature instead of chemical denaturation
MethodsX, 2014 Comet-FISH technique is an extension of commonly used comet assay. Its purpose is to determine whether primary DNA damage which comet assay detects occurred within a sequence of interest that is visualized by hybridization of fluorescent probe.
Marin Mladinic, Davor Zeljezic
doaj +1 more source
Closing the gap: from public concerns about health effects to science-based testing of chemicals
Frontiers in Genetics, 2015 The comet assay is a powerful technology for measuring DNA integrity in humans and other organisms at the level of individual cells. The assay is used for research and industrial testing but suffers from a lack of standardisation, which hinders its use ...
Sergey Shaposhnikov +2 more
doaj +1 more source
The Comet Assay: Tails of the (Un)expected. Use of the comet assay in pharmaceutical development.
Frontiers in Genetics, 2015 In genotoxicity testing of pharmaceuticals the rodent alkaline comet assay is being increasingly used as a second in vivo assay in addition to the in vivo micronucleus assay to mitigate in vitro positive results as recommended by regulatory guidance.
Bas-jan Van Der Leede
doaj +1 more source
The basic principles of DNA damage detection by the alkaline comet assay [PDF]
Arhiv za farmacijuThe Comet assay is a highly sensitive and rapid method for detecting DNA damage at the level of individual cells. It measures single-stranded and double-stranded DNA breaks, alkali-labile, incomplete excision repair and cross-linking sites.
Živković Lada +6 more
doaj +1 more source
The use of comet assay to assess global DNA methylation in human biomonitoring studies.
Frontiers in Genetics, 2015 The Comet assay is a valuable tool for the detection of DNA damage in genotoxicity and human biomonitoring studies. Throughout the years, this biomarker has undergone several adaptations in their protocol in order to increase its sensitivity and the ...
Carla Costa +2 more
doaj +1 more source
Citation: 'comet assay' in the IUPAC Compendium of Chemical Terminology, 5th ed.; International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.15576 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms.
Solange Costa +2 more
+5 more sources
HiComet: a high-throughput comet analysis tool for large-scale DNA damage assessment
BMC Bioinformatics, 2018 Background DNA damage causes aging, cancer, and other serious diseases. The comet assay can detect multiple types of DNA lesions with high sensitivity, and it has been widely applied.
Taehoon Lee +7 more
doaj +1 more source
Toxoplasma gondii infection can induce retinal DNA damage:an experimental study
International Journal of Ophthalmology, 2014 AIM: To detect whether Toxoplasma gondii (T. gondii) infection of mice can induce retinal DNA damage.METHODS: A total of 20 laboratory-bred male Swiss albino mice were used and divided into four groups:control group (non-infected animals); T ...
Nagwa Mostafa El-Sayed, Eman Mohamed Aly
doaj +1 more source
Relationship between CpG and non-CpG DNA methylation in human lymphocytes assessed with comet assay [PDF]
The Ukrainian Biochemical JournalTissue-specific DNA methylation plays an important role in the regulation of many functional processes. The methylation level in single cells can be assessed using the comet assay (single-cell gel electrophoresis), a simple and cost-effective technique ...
M. Chopei +3 more
doaj +1 more source