Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. [PDF]
Nat Protoc, 2020 The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems.
Møller P +30 more
europepmc +5 more sources
, 2022 S.2Actually, precision cut lung slices are under extensive investigation to serve as a model for acute inhalation toxicology. Slices can be employed as an ex-vivo/in-vitro model as alternative method in the context of REACH and the 3Rs. Moreover, this in
Sewald, K. +3 more
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Stochastic modeling for the COMET-assay [PDF]
, 2003 We present a stochastic model for single cell gel electrophoresis (COMET-assay) data. Essential is the use of point process structures, renewal theory and reduction to intensity histograms for further data ...
Hösel, V. +4 more
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Impact of (poly)phenol-rich dietary sources on DNA damage: insights from human intervention studies using the Comet assay - a review and perspective. [PDF]
Br J Nutr(Poly)phenols are plant-derived food bioactives abundantly present in human diet. They exert positive effects on various aspects of human health and in particular in reducing the risk of chronic non-communicable diseases.
Milev M +5 more
europepmc +3 more sources
Genotoxicity of nitroso compounds and sodium dichromate in a model combining organ cultures of human nasal epithelia and the comet assay [PDF]
, 2001 Genotoxic effects of xenobiotics are a possible step in tumor initiation in the mucosa of the upper aerodigestive tract. Using the comet assay, detecting genotoxicity in human tissue has been restricted to single incubations in vitro, but in vivo most ...
Bergner, Albrecht +7 more
core +1 more source
Detection of DNA damage by alkaline comet assay in mouse colonic mucosa
STAR Protocols, 2021 Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin.
Elisa Boutet-Robinet +4 more
doaj +1 more source
Comparative Evaluation of Genotoxicity of Captan in Amphibian Larvae (Xenopus laevis and Pleurodeles waltl) Using the Comet Assay and the Micronucleus Test [PDF]
, 2006 Captan (N-trichloromethylthio-4-cyclohexene-1,2-dicarboximide) is a fungicide used to inhibit the growth of many types of fungi on plants used as foodstuffs.
Gauthier, Laury +8 more
core +1 more source
Using a medium-throughput comet assay to evaluate the global DNA methylation status of single cells
Frontiers in Genetics, 2014 The comet assay is a simple and cost effective technique, commonly used to analyse and quantify DNA damage in individual cells. The versatility of the comet assay allows introduction of various modifications to the basic technique.
Angelique eLewies +5 more
doaj +1 more source
Frontiers in Genetics, 2014 Sperm DNA damage is associated with fertilization failure, impaired pre-and post- embryo implantation and poor pregnancy outcome. A series of methodologies to assess DNA damage in spermatozoa have been developed but most are unable to differentiate ...
Elva I eCortes-Gutierrez +5 more
doaj +1 more source
Antioxidants and the Comet assay
Mutation Research/Reviews in Mutation Research, 2009 It is widely accepted that antioxidants, either endogenous or from the diet, play a key role in preserving health. They are able to quench radical species generated in situations of oxidative stress, either triggered by pathologies or xenobiotics, and they protect the integrity of DNA from genotoxicants.
Cemeli, Eduardo +2 more
openaire +2 more sources