Results 11 to 20 of about 33,064 (290)
Is the Comet Assay a Sensitive Procedure for Detecting Genotoxicity? [PDF]
Journal of Nucleic Acids, 2010 Although the Comet assay, a procedure for quantitating DNA damage in mammalian cells, is considered sensitive, it has never been ascertained that its sensitivity is higher than the sensitivity of other genotoxicity assays in mammalian cells. To determine
Satomi Kawaguchi +4 more
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International Journal of Scientific and Technological Research, 2019 Currently, humans and other living things can have many genotoxic damages due to reasons such as chemicals, drugs, unfavorable environmental effects. Breaks in the DNA structure due to genotoxic damage can cause mutations, changes in chromosome structure and cancer.
Cücer, Nurhan +3 more
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Bio-Protocol, 2013 The Comet assay (or Single Cell Gel Electrophoresis assay) is a sensitive technique to detect DNA damage at the level of an individual cell. This technique is based on micro-electrophoresis of cells DNA content.
Elisa Boutet-Robinet +2 more
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Haseki Tıp Bülteni, 2016 Aim: There are several clinical and cytogenetic studies that anesthetics cause choromosomal changes. We report data on the possible genotoxic properties of two inhalational anaesthetics, sevoflurane and desflurane in lymphocytes of children evaluated for
Yeşim Işıkçı +5 more
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The Comet Assay: Tails of the (Un)expected. Use of the comet assay in pharmaceutical development.
Frontiers in Genetics, 2015 In genotoxicity testing of pharmaceuticals the rodent alkaline comet assay is being increasingly used as a second in vivo assay in addition to the in vivo micronucleus assay to mitigate in vitro positive results as recommended by regulatory guidance.
Bas-jan Van Der Leede
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The comet assay – from toy to tool
Frontiers in Genetics, 2015 The comet assay is nowadays the most common method for measuring DNA damage and repair in single cells. It is based on the microelectrophoretic study published by Ostling and Johanson (1984) and was developed by Singh and coworkers (1988) to a versatile ...
Guenter Speit
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Comet assay on mice testicular cells
Frontiers in Genetics, 2015 Heritable mutations may result in a variety of adverse outcomes including genetic disease in the offspring. In recent years the focus on germ cell mutagenicity has increased and the “Globally Harmonized System of Classification and Labelling of ...
Anoop Kumar Sharma
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Modelling the comet assay [PDF]
BMC Systems Biology, 2007 The single-cell gel electrophoresis technique or comet assay is widely regarded as a quick and reliable method of analysing DNA damage in individual cells. It has a proven track record from the fields of biomonitoring to nutritional studies.
Darragh G. McArt +3 more
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Comet Assay measurements: a perspective [PDF]
Cell Biology and Toxicology, 2007 The Comet Assay or single cell gel electrophoresis assay is one of the very widely used assays to microscopically detect DNA damage at the level of a single cell. The determination of damage is carried out either through visual scoring of cells (after classification into different categories on the basis of tail length and shape) or by using different ...
Kumaravel, T. S. +3 more
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An Overview of Comet Assay Application for Detecting DNA Damage in Aquatic Animals
Agriculture, 2023 This review discusses several research studies that employed comet assay to evaluate the environmental impact of genotoxins in aquatic environments. It focuses on in vivo and in situ studies of aquatic animals.
Nan Jiang +10 more
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