Results 41 to 50 of about 131,362 (199)
FEBS Open Bio, EarlyView.Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
Competències mediàtiques a l’IES Dr. Balmis d’Alacant
Ítaca, 2013 La introducció de les TIC en l’ensenyament (les TAC) planteja molts interrogants entre els membres de la comunitat educativa, especialment entre el professorat.
Josep Manuel Escolano i López
doaj +1 more source
Development of human monoclonal antibodies against TARM1 by yeast display
FEBS Open Bio, EarlyView.Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
Consolidació dels consells consultius a Espanya i el seu paper com a garants de l'autonomia
Revista Catalana de Dret Públic, 2009 Aquest treball analitza el procés de creació i consolidació dels òrgans consultius com una manifestació important del desplegament de l'Estat autonòmic i de la descentralització del poder a Espanya, que té el seu fonament en les potestats d ...
Juan Cano Bueso
doaj
Revista d'Innovació i Recerca en Educació, 2010 Ressenya del llibre: Desarrollo de competencias: Teoría y práctica. Balance, proyecto profesional y aprendizaje basado en el trabajo.
Anna Forés-Miravalles
doaj +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
L'avaluació de les competències transversals a l'ensenyament universitari
Revista d'Innovació i Recerca en Educació, 2010 L’article recull i sistematitza les propostes sorgides al seminari sobre Avaluació de les Competències Transversals. S’hi analitzen les dimensions de la competència, en especial el component actitudinal que hi incorporen, i es fan suggeriments per a l ...
Nati Cabrera-Lanzo +1 more
doaj +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source