Results 51 to 60 of about 2,770,096 (297)

Thermodynamics of competitive molecular channel transport: application to artificial nuclear pores. [PDF]

PLoS ONE, 2010
In an analytical model channel transport is analyzed as a function of key parameters, determining efficiency and selectivity of particle transport in a competitive molecular environment.
Wolfgang R Bauer, Walter Nadler
doaj   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Apparent dissociation constants for the binding of different sugars to MhPA14, using the competitive binding assay.

, 2019
Apparent dissociation constants for the binding of different sugars to MhPA14, using the competitive binding assay.
Peter L. Davies (121653), Shayan Assaie-Ardakany (7027148), Brigid Conroy (7027151), Shuaiqi Guo (121638), Tyler D. R. Vance (3365498)   +4 more
core   +1 more source

Characterisation of the Binding Properties of Bacillus Thuringiensis 18 Toxin on Leukaemic Cells

Journal of Experimental & Clinical Cancer Research, 2010
Background Various strains of Bacillus thuringiensis (Bt) have been found to produce parasporal proteins that are cytotoxic to human cancer cells. This study aims to establish the binding affinity of purified Bt 18 toxin for CEM-SS (T lymphoblastic ...
Nadarajah Vishna D, Mohamed Shar M, Wong Rebecca SY, Tengku Ibrahim   +3 more
doaj   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Noise in case of competitive TF binding was higher compared to single TF regulation across a wide range of parameter values.

, 2022
Changes in mRNA and protein synthesis rates, mRNA and protein degradation rates, on- and off-rate parameters (λon and λoff respectively) changed mean expression levels both in single TF and competitive TF binding, but the noise levels in competitive ...
Lavisha Parab (14248215), Sampriti Pal (8630859), Riddhiman Dhar (3323460)   +2 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Contribution of fibronectin-binding protein to pathogenesis of Streptococcus suis serotype 2 [PDF]

, 2002
In the present study we investigated the role of the fibronectin (FN)- and fibrinogen (FGN)-binding protein (FBPS) in the pathogenesis of Streptococcus suis serotype 2 in piglets. The complete gene encoding FBPS from S.
Dijkstra, J., Smit, H.E., Verhaar, R., Alphen, L., van, Greeff, A., de, Robin Verhaar, Loek van Alphen, Herma Buys, Buys, H., Janny Dijkstra, Hilde E. Smith, Astrid de Greeff   +11 more
core   +1 more source

6-Arylpyrido[2,3-d]pyrimidines as novel ATP-competitive inhibitors of bacterial D-alanine:D-alanine ligase. [PDF]

PLoS ONE, 2012
BACKGROUND: ATP-dependent D-alanine:D-alanine ligase (Ddl) is a part of biochemical machinery involved in peptidoglycan biosynthesis, as it catalyzes the formation of the terminal D-ala-D-ala dipeptide of the peptidoglycan precursor UDPMurNAc ...
Veronika Škedelj, Emilija Arsovska, Tihomir Tomašić, Ana Kroflič, Vesna Hodnik, Martina Hrast, Marija Bešter-Rogač, Gregor Anderluh, Stanislav Gobec, Julieanne Bostock, Ian Chopra, Alex J O'Neill, Christopher Randall, Anamarija Zega   +13 more
doaj   +1 more source

RNA‐related functions of BRCA2 in transcription, replication, and genome stability

FEBS Letters, EarlyView.
Beyond its role in homologous recombination, BRCA2 regulates R‐loop homeostasis, DNA–RNA hybrid resolution, RNA polymerase II dynamics, and transcriptional activation. Disruption of any of these functions can contribute to genome instability, inflammatory signaling, and tumorigenesis. BRCA2 is a central tumor suppressor protein best known for its roles
Lucia Alvaro‐Aranda, Sreerama Chaitanya Sridhara, Aura Carreira   +2 more
wiley   +1 more source