Results 211 to 220 of about 53,639 (248)

AN INDIRECT COMPETITIVE ELISA FOR DETERMINATION OF CITRININ

Journal of Food Safety, 2011
Citrinin (CIT)–protein artificial antigen was prepared by conjugating CIT with keyhole limpet hemocyanin by 1,4-butanediol diglycidyl ether. By immunization and fusion, a hybridoma cell line named K2-F3, which stably secreted the monoclonal antibody (McAb) against CIT was obtained.
YONGNING LI, YUANYUAN WANG, YANGHAO GUO
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Competitive ELISA for the measurement of tau protein in Alzheimer's disease

Journal of Immunological Methods, 1990
Tau protein is a major component of paired helical filaments (PHFs) which constitute the characteristic neurofibrillary tangle lesions observed in Alzheimer's disease. Two tau mAbs have been produced which show distinct patterns of immunoreactivity with intact human tau and with tau incorporated in PHFs. The mAb 423 recognises PHFs but not human tau on
C R, Harrington, P C, Edwards, C M, Wischik   +2 more
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A competitive inhibition ELISA for the quantification of human interferon-γ

Journal of Immunological Methods, 1993
A competitive enzyme immunoassay has been developed for the measurement of human interferon-gamma (IFN-gamma) in cell culture supernatants. The assay is based on the dose-dependent inhibitory effect of liquid phase IFN-gamma on the binding of a specific monoclonal antibody to recombinant IFN-gamma (rIFN-gamma) immobilized on microtitre plate wells. The
A B, Wilson, S M, McHugh, J, Deighton, P W, Ewan, P J, Lachmann   +4 more
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Improved competitive enzyme-linked immunoassay (ELISA) for albuminuria

Clinica Chimica Acta, 1989
Microalbuminuria has been established as a marker strongly predictive of diabetic nephropathy. The appearance of microalbuminuria (30-150 micrograms/min) is considered to herald incipient nephropathy, and the progression to clinical proteinuria (greater than 200 mg/24 h) is believed to reflect a shift from reversible to irreversible renal damage in ...
R G, Neuman, M P, Cohen
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P450 aromatase inhibition assay using a competitive ELISA

Journal of Pharmaceutical and Biomedical Analysis, 2005
P450 aromatase (P450arom) is a well known target by anti-cancer drugs and toxic chemicals and efficient and convenient analytical tools are desired for. We established a convenient assay for P450arom inhibition based on an enzyme-linked immunosorbent assay (ELISA).
Kazuhiro, Matsui, Shigeaki, Nishii, Masanori, Oka   +2 more
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Competitive ELISA for the Detection and Quantification of Mycobacterium ulcerans Mycolactone

2021
Lipids and other hydrophobic analytes are difficult to quantify by routine immunoassays due to the need to use aqueous buffers. Here, we describe an ELISA protocol suitable for the detection of mycolactone, the polyketide toxin of Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU).
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Precision, Limit of Detection and Range of Quantitation in Competitive ELISA

Analytical Chemistry, 2004
This paper develops a mathematical model for describing the within-plate variation as the RSD (relative standard deviation) of absorbance measurements in a wide concentration range in competitive ELISA and proposes a method for determining the limit of detection (LOD) and range of quantitation (ROQ).
Yuzuru, Hayashi, Rieko, Matsuda, Tamio, Maitani, Kazuhiro, Imai, Waka, Nishimura, Katsutoshi, Ito, Masako, Maeda   +6 more
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Application of experimental design techniques to optimize a competitive ELISA

Journal of Immunological Methods, 1996
This paper describes the application of experimental design techniques to optimize a sensitive ELISA for a hapten molecule with a calibration range of 0-1000 pg/ml. Ten factors that were expected to affect the assay performance were initially screened, followed by factorial experiments to delineate the effects of the critical factors identified at the ...
G S, Sittampalam, W C, Smith, T W, Miyakawa, D R, Smith, C, McMorris   +4 more
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A competition ELISA for the detection of mycobacterial antigen in tuberculosis exudates

Tubercle, 1988
An enzyme-linked immunosorbent assay (ELISA) was designed to detect the inhibition of BCG-anti-BCG reactions by soluble antigens of Mycobacterium tuberculosis. Eighty-four samples were tested of which 59 were of cerebrospinal fluid (CSF) (25 control, 10 bacterial, 14 viral and 10 tuberculous meningitis); and twenty-five were of pleural or ascitic fluid
A, Ramkisson, Y M, Coovadia, H M, Coovadia   +2 more
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Uncertainty of measurement for competitive and indirect ELISAs.

Revue scientifique et technique (International Office of Epizootics), 2008
A method for the estimation of the uncertainty of measurements for Gaussian outcomes of enzyme-linked immunosorbent assay (ELISA) is described using competitive and indirect foot and mouth disease (FMD) ELISAs. Assay repeatability was determined by random effects analysis of variance, and the normality of the residuals was checked.
J F, Toussaint, P, Assam, B, Caij, F, Dekeyser, K, Knapen, H, Imberechts, N, Goris, G, Molenberghs, K, Mintiens, K, De Clercq   +9 more
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