Results 201 to 210 of about 53,639 (248)
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2003
Enzyme-linked immunosorbent assay (ELISA) is a very useful technique for the specific and sensitive assay of certain compounds, in which suitable antibodies, monoclonal or polyclonal, to the compounds are available. The technique has found particular application in the monitoring of environmental contaminants and toxins, either studying the primarily ...
K, Makarananda, G E, Neal
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Enzyme-linked immunosorbent assay (ELISA) is a very useful technique for the specific and sensitive assay of certain compounds, in which suitable antibodies, monoclonal or polyclonal, to the compounds are available. The technique has found particular application in the monitoring of environmental contaminants and toxins, either studying the primarily ...
K, Makarananda, G E, Neal
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A competitive ELISA for the serodiagnosis of hypodermosis
Veterinary Parasitology, 1997Hypodermosis is a parasitic disease of cattle caused by Hypoderma lineatum and H. bovis. It is an important health and welfare problem of infested cattle and a cause of considerable economic loss. In live animals, detection can be either by direct clinical examination of infested cattle and palpation of second and third stage larvae in the back or by ...
K A, Webster, M, Giles, C, Dawson
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vWf inhibitor detection by competitive ELISA
Journal of Immunological Methods, 1997An inhibitor to von Willebrand factor (vWf) was detected in the plasma from two patients with histories of mild bleeding and one patient with a severe deficiency in the Factor VIII complex using a competitive enzyme-linked immunosorbent assay (ELISA) procedure.
M W, Stewart +3 more
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A Competitive ELISA for Albumin in Rat Urine
Journal of Immunoassay and Immunochemistry, 2004Nephropathy is characterized by urinary micro albumin. Rats are usually used in experimental studies. But, there was no specific and simple method for detecting rat urinary albumin. A specific, easily performed, and sensitive method for quantitatively determining rat urinary albumin is needed.
Xiaorui, Chen +2 more
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Development of an indirect competitive ELISA for the determination of papaverine
Talanta, 2005Papaverine (1-(3,4-dimethoxybenzyl)-6,7-dimethoxyisoquinoline, PAP) is a member of the benzylisoquinoline sub-group of the opium alkaloids. It has been widely used for treating diseases like pulmonary arterial embolism and renal or biliary colic. In this paper, a specific conjugate of mono-demethylated papaverine-O-carboxylmethyl ether (MDMPAP-O-CME ...
Jin, Yan +5 more
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Multichannel electroanalytical devices for competitive ELISA of phenylethanolamine A
Biosensors and Bioelectronics, 2018Herein, we reported a disposable electroanalytical device for competitive enzyme-linked immunosorbent assay (ELISA) of phenylethanolamine A (PA). Conductive carbon tape coated with gold cluster served as the working electrode, while the counter and reference electrodes were fabricated on the filter paper by the screen-printing technique.
Yang, Liu +10 more
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Development of Competitive Direct ELISA for Gossypol Analysis
Journal of Agricultural and Food Chemistry, 2005Anti-gossypol monoclonal antibody was purified from cell culturing supernatant by ammonium sulfate precipitation and Protein A AffinityPak. The antigen (i.e., gossypol) was labeled with horseradish peroxidase through Schiff-base formation. Both the purified antibody and the enzyme-labeled gossypol were employed to develop a competitive direct enzyme ...
Jing, Wang +5 more
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Statistical package for analysis of competition ELISA results
Journal of Immunological Methods, 1981A computation and analysis program has been assembled to facilitate the use of competition ELISA and similar assays for studies of antigen regulation and turnover. The program fits sigmoidal standard curves using a 4-parameter logistic function, determines amounts of antigen from the equation which defines the standard curve, and calculates specific ...
P F, Canellas, A E, Karu
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Indirect Competitive Enzyme-Linked Immunosorbent Assay (ELISA)
Cold Spring Harbor Protocols, 2017The indirect competitive ELISA (indirect cELISA) pits plate-immobilized antigen against antigens in solution for binding to antigen-specific antibody. The antigens in solution are in the test sample and are first incubated with antigen-specific antibody.
Thomas O, Kohl, Carl A, Ascoli
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Analysis of Antibody Inhibition of PfEMP1 Binding by Competition ELISA
2022Plasmodium falciparum express variant antigens on the surface of infected erythrocytes (IEs). These include proteins of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. PfEMP1 proteins mediate binding of IEs to various human endothelial receptors and induce antibodies response during natural infections.
Rebecca W. Olsen +2 more
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