Results 71 to 80 of about 53,639 (248)

Using of competitive ELISA in detection of brucella antibodies in cattle sera [PDF]

Iraqi Journal of Veterinary Sciences, 2009
Competitive ELISA test was used in this study to identify brucella antibodies in cattle sera in Mosul, and assessing the results that are given by Rose-Bengal test.
M. I. Al-Farwachi, O. M. Al-Iraqi, O. Kh. Al-Hankawe, M. O. Abdul-Majeed   +3 more
doaj  

Detection of RNA Modifications by HPLC Analysis and Competitive ELISA [PDF]

, 2014
Over 100 different RNA modifications exist that are introduced posttranscriptionally by enzymes at specific nucleotide positions. Ribosomal RNA (rRNA) and transfer RNA (tRNA) exhibit the most and diverse modifications that presumably optimize their structure and function.
Nees, Gernot, Kaufmann, Andreas, Bauer, Stefan   +2 more
openaire   +2 more sources

Microglia‐Targeted Biomimetic Tetrahedral Framework Nucleic Acid Nanovesicles for Synergistic Treatment of Sepsis‐Associated Encephalopathy

Advanced Science, EarlyView.
Sepsis‐associated encephalopathy (SAE) lacks effective therapies. We developed ME@FDsi, a biomimetic nanodrug using a tetrahedral framework nucleic acid to deliver disulfiram and siTNFα. It crosses the blood‐brain barrier, targets M1 microglia, inhibits pyroptosis and inflammation, and scavenges ROS.
Huimin Shi, Qiuxia Gao, Wenying Wang, Bin Li, Yukun Chen, Zhijun Yao, Yujie Li, Junrui Li, Na Li, Gong Gu, Zhimin Hou, Mengyuan Yang, Ruilin Zhang, Hongju Yang, Yuhui Liao, Hongyi Lei   +15 more
wiley   +1 more source

Development of a Human 3D Immune‐Competent Neurovascular Model Enabling Time‐Resolved Monitoring of Neuroinflammatory Dynamics and Neuroimmune Interactions

Advanced Science, EarlyView.
Neuroinflammation alters blood–brain barrier integrity and contributes to neurological disorders, yet existing models lack human immune complexity. This study presents a 3D, immune‐responsive platform that reconstructs key neurovascular components and enables real‐time monitoring.
Pavlo Gordiichuk, Jing Bai, Olurotimi A. Bolonduro, Andrew M. Silverman, Robert Madison Green, Jason H. Lasser, Paul W. Fleming, Lishomwa C. Ndhlovu, James F. Saunders, Dmitry Shvartsman   +9 more
wiley   +1 more source

Biosynthesis of Sciadonic Acid Derived from Gymnosperms with Anti‐Colitis Activity

Advanced Science, EarlyView.
This study establishes an efficient Yarrowia lipolytica platform for unusual sciadonic acid (SCA) production and assess the anti‐colitis efficacy in mice of SCA, supporting its potential as a novel therapeutic lipid. ABSTRACT Sciadonic acid (SCA), Δ5‐unsaturated fatty acid with anti‐inflammatory and lipid‐regulatory properties, is predominantly derived
Yadi Gao, Wei Liu, Xiuping Liang, Jie Bai, Hong Chang, Wenchao Chen, Mohamed A. Farag, Jianbo Xiao, Huifeng Jiang, Jiasheng Wu, Lili Song   +10 more
wiley   +1 more source

Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay

Journal of Nanobiotechnology, 2019
Background Sensitive and specific antibodies can be used as essential probes to develop competitive enzyme-linked immunosorbent assay (cELISA).
Yamin Sheng, Kun Wang, Qizhong Lu, Pinpin Ji, Baoyuan Liu, Jiahong Zhu, Qingyuan Liu, Yani Sun, Jingfei Zhang, En-Min Zhou, Qin Zhao   +10 more
doaj   +1 more source

Integrated Single‐Cell and Spatial Analysis Reveals a Metabolic‐Immune Axis Driving Aortic Dissection

Advanced Science, EarlyView.
Single‐cell and spatial profiling of 110 human thoracic aortic samples reveals a stromal–immune circuit driving aortic dissection. An elastin‐rich fibroblast subset is depleted with age and markedly reduced in disease, weakening aortic wall integrity.
Jing Tao, Huanjie Yang, Jiahui Yong, Xueting Chen, Qiang Zhao, Xueli Wu, Lei Yan, Li Pang, Fan Luo, Mengjun Yu, Shanshan Pan, Deyang Li, Rouxi Chen, Juan Wang, Zhensheng Dong, Fan Yang, Yue Wang, Yang Chen, Hongjian Zheng, Zhimin Yang, Zijie Wang, Karsten Kristiansen, Hui Peng, Xiaodong Fang, Juan Shen, Yining Yang   +25 more
wiley   +1 more source

Two nanobody-based immunoassays to differentiate antibodies against genotype 1 and 2 porcine reproductive and respiratory syndrome virus

Animal Diseases
Porcine reproductive and respiratory syndrome virus (PRRSV) infection causes significant economic loss to the global pig industry. Genotype 1 and 2 PRRSV (PRRSV-1 and -2) infections have been reported in China, Europe and America. For accurate prevention,
Xu Chen, Yueting Chang, Lu Zhang, Xinyu Zhao, Zhihan Li, Zhijie Zhang, Pinpin Ji, Qingyuan Liu, Jiakai Zhao, Jiahong Zhu, Baoyuan Liu, Xinjie Wang, Yani Sun, Qin Zhao   +13 more
doaj   +1 more source

Establishment and Preliminary Application of Highly Sensitive Competitive ELISA for Folic Acid

Shipin gongye ke-ji
In order to achieve high sensitivity and rapid detection of folic acid, this study established a highly sensitive competitive enzyme-linked immunosorbent assay (cELISA) by preparing folic acid monoclonal antibody and optimizing the detection system.
Yao WANG, Yanfei LI, Jinbo CAO, Xiyan YANG, Jiaxiang HUANG, Mingyue HUANG, Lu HAN, Kaile WANG, Xiujin CHEN, Zhaozhou LI, Min ZHANG, Huaibin KANG   +11 more
doaj   +1 more source

Detection and Quantitation of Gluten in Fermented-Hydrolyzed Foods by Antibody-Based Methods: Challenges, Progress, and a Potential Path Forward

Frontiers in Nutrition, 2019
Celiac disease (CD) affects ~1 in 141 individuals in the United States, requiring adherence to a strict gluten-free diet. The Codex Standard and the European Commission states that gluten level of gluten-free foods must not exceed 20 ppm.
Rakhi Panda, Eric A. E. Garber
doaj   +1 more source