Results 91 to 100 of about 46,344 (111)

Enzymic activity of the second component of complement

Biochemistry, 1975
Isolated C2 and C2i preparations were able to hydrolyze a number of synthetic esters containing basic amino acids, among which N-alpha-acetylglycyl-L-lysine methyl ester (AcGlyLysOMe) was most susceptible. The cleaving activity was a property of the C2 molecule, since it correlated with the presence of C2 on analyses of C2 preparations by ...
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Complement activation by antibodies to DNA in systemic lupus erythematosus measured by enzyme immunoassay

Clinical Immunology and Immunopathology, 1985
An enzyme immunoassay to detect complement-fixing antibodies to DNA (CF-antiDNA) was developed. Of SLE sera, 64% had these antibodies as did 6% of 50 rheumatoid arthritis and 3.2% of 93 normal human sera. The mean CF-antiDNA level was higher in the sera of SLE patients with renal disease than those SLE patients who had no renal disease (P less than 0 ...
R R, Valle, R B, Eaton, G, Schnneider, P H, Schur   +3 more
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Activated complement factor 3 is associated with liver fat and liver enzymes: the CODAM study

European Journal of Clinical Investigation, 2013
AbstractBackgroundThe complement system may be involved in the pathogenesis of alcoholic and nonalcoholic liver disease, although studies in humans are scarce. For this reason, we investigated whether circulating levels of activated complement factor 3 (C3a) were associated with hepatic steatosis and hepatocellular damage.Materials and methodsPlasma ...
Wlazlo, N., van Greevenbroek, M.M.J., Ferreira, I., Jansen, E.H.J.M., Feskens, E.J.M., van der Kallen, C.J.H., Schalkwijk, C.G., Bravenboer, B., Stehouwer, C.D.A.   +8 more
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Measurements of β-Arrestin Recruitment to Activated Seven Transmembrane Receptors Using Enzyme Complementation

2012
The recruitment of arrestins to activated 7TMRs results in the activation of alternative signaling pathways, quenching of G-protein activation, and coupling to clathrin-mediated endocytosis. The nearly ubiquitous involvement of arrestin in 7TMR signaling has spurred the development of several methods for monitoring this interaction in mammalian cells ...
Daniel L, Bassoni, William J, Raab, Philip L, Achacoso, Chin Yee, Loh, Tom S, Wehrman   +4 more
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Complement activation by PVA as measured by ELIFA (enzyme-linked immunoflow assay) for SC5b-9

Biomaterials, 2000
Polyvinyl alcohol (PVA) coated onto polyethylene (PE) tubes exposed to human serum for 1 hour at 37 degrees C resulted in the production of 1.03 +/- 0.04 microg/cm2 of the soluble form of the terminal membrane attack complex, SC5b-9. This was approximately 20 x that produced by the polyethylene. About one quarter of this total was found associated with
J P, Black, M V, Sefton
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Enzyme Fragment Complementation Binding Assay for p38α Mitogen-Activated Protein Kinase to Study the Binding Kinetics of Enzyme Inhibitors

ASSAY and Drug Development Technologies, 2006
The majority of protein kinase assays used in drug discovery research are enzyme activity assays. These assays are based on the measurement of phosphorylated protein or peptide substrate, which is the end product of the enzyme reaction. Since most kinase inhibitors are ATP competitive, prediction of the activity of compounds in cellular systems based ...
Guido J R, Zaman, Miranda M C, van der Lee, Jacobus J, Kok, Rob L H, Nelissen, Elma E M G, Loomans   +4 more
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DNA repair enzyme deficiency and in vitro complementation of the enzyme activity in cell-free extracts from ataxia telangiectasia fibroblasts

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
Three ataxia telangiectasia homozygotes, one heterozygote and normal fibroblast strains were compared as to the capacity of their cellular extracts to enhance the priming activity of gamma-irradiated colicin E1 DNA for purified DNA polymerase (EC 2.7.7.7) of Escherichia coli.
T, Inoue, A, Yokoiyama, T, Kada
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Effects of activated complement components on enzyme secretion by macrophages.

Immunology, 1977
Purified cleavage products of the guinea-pig complement component C3, namely C3b and C3a, interact with guinea-pig and mouse macrophages in culture to induce a dose- and time dependent release of lysosmal enzymes into the medium. In the case of C3b the selectivity of the release of hydrolases, which occurs without cell killing, is shown by ...
H U, Schorlemmer, A C, Allison
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Binding and activation of C4 and C3 on the red cell surface by non-complement enzymes

Molecular Immunology, 1988
We investigated the binding of C4 and C3 to red cell surfaces by non-complement enzymes. Cell bound C components were quantitated by a radioimmunoassay, the chain structure of bound components was analyzed by Western blotting and the hemolytic activity of bound components was determined.
M, Kirschfink, T, Borsos
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Digestion of the fifth component of complement by eosinophil lysosomal enzymes

Virchows Archiv B Cell Pathology Including Molecular Pathology, 1981
Recently our laboratory has shown that neutrophils contain enzymatic activity within their lysosomal granules which will generate chemotactic activity for neutrophils and tumor cells from the fifth component of complement (C5). We have now expanded this initial observation and have demonstrated that eosinophils can release enzymatic activity from their
H, Ogawa, S L, Kunkel, J C, Fantone, P A, Ward   +3 more
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