Results 1 to 10 of about 1,533 (97)

Purification of the human complement control protein C3b inactivator [PDF]

Biochemical Journal, 1980
An alternative method of isolation from human plasma is described for C3b inactivator, C3bINA, the proteinase that in conjunction with either beta 1H or C4b-binding protein will hydrolyse respectively C3b or C4b, the activation products of the third, C3 and fourth, C4, components of complement. The purification is by chromatography of plasma on columns
Lisa Crossley, Roy Porter
openalex   +4 more sources

Human complement C3b inactivator: isolation, characterization, and demonstration of an absolute requirement for the serum protein beta1H for cleavage of C3b and C4b in solution. [PDF]

The Journal of experimental medicine, 1977
The complement regulatory enzyme, C3b inactivator (C3bINA), has been purified from human serum by affinity chromatography on an anti-C3bINA Sepharose column. Subsequent chromatography on DEAE-cellulose and removal of IgG with anti-IgG Sepharose resulted in a product which was found to be homogeneous by polyacrylamide gel electrophoresis at pH 8.9 and ...
Michael K. Pangburn, Robert D. Schreiber, Hans J. Müller‐Eberhard   +2 more
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Molecular Interactions of the Control Proteins C3b Inactivator and β1H with Various Complement Intermediates [PDF]

The Journal of Immunology, 1978
Abstract Radioiodinated β1H was demonstrated to bind to sheep EAC4b, EAC4,2,3b and EC3b, but not to E, EA, EAC4,2 or to C3bINA and β1H treated EC3b. This binding was dependent upon the C3b concentration on the surface of the cells and could be inhibited by unlabeled β1H, free C3b or high concentrations of Factor B.
Michael K. Pangburn, Robert D. Schreiber, Hans J. Müller‐Eberhard   +2 more
openalex   +2 more sources

The fourth component of human complement treated with amines or chaotropes or frozen-thawed (C4b-like C4): interaction with C4 binding protein and cleavage by C3b/C4b inactivator. [PDF]

The Journal of Immunology, 1982
Abstract We have shown previously that C4 treated with amines or chaotropes, although uncleaved, exhibits properties that are similar to C4b. Studies by other groups suggest that this C4b-like form of C4 is characterized by the lack of an internal thiolester bond that is present in native C4. We report here that C4 treated
I. von Zabern, Ernest Bloom, V. Chu, Irma Gigli   +3 more
openalex   +3 more sources

Borrelia recurrentis employs a novel multifunctional surface protein with anti-complement, anti-opsonic and invasive potential to escape innate immunity [PDF]

, 2009
Borrelia recurrentis, the etiologic agent of louse-borne relapsing fever in humans, has evolved strategies, including antigenic variation, to evade immune defence, thereby causing severe diseases with high mortality rates.
AG Barbour, AG Barbour, AG Barbour, AG Barbour, AS Coleman, C Neeleman, C Siegel, C Skerka, CA Brissette, Christiane Brenner, CS Brooks, D Raoult, David M. Ojcius, DJ Grab, E Rossmann, E Rossmann, GD Wieland, H Fuchs, J Bunikis, JA Gebbia, JC Scott, JC Setubal, JL Coleman, JV McDowell, K Hartmann, KM Hovis, KM Hovis, KO Sundnes, L Houhamdi, Markus M. Simon, MD Kramer, Melanie Schott, MK Pangburn, NM Ras, P Kraiczy, P Kraiczy, P Kraiczy, P Kraiczy, P Kraiczy, P Kraiczy, P Stewart, Peter F. Zipfel, Peter Kraiczy, PF Zipfel, PF Zipfel, PM Southern, RD Horstmann, Reinhard Wallich, S Hammerschmidt, S Kuhn, S Kuhn, S Poltermann, S Ram, Sally J. Cutler, SH Rooijakkers, SJ Cutler, Sonja Grosskinsky, T Meri, T Meri, WR Zuckert   +59 more
core   +9 more sources

Diversity of Sites for Measles Virus Binding and for Inactivation of Complement C3b and C4b on Membrane Cofactor Protein CD46 [PDF]

Journal of Biological Chemistry, 1995
The complement system membrane cofactor protein (MCP) CD46 serves as a C3b/C4b inactivating factor for the protection of host cells from autologous complement attack and as a receptor for measles virus (MV). MCP consists of four short consensus repeats (SCR) which are the predominant extracellular structural motif.
K, Iwata, T, Seya, Y, Yanagi, J M, Pesando, P M, Johnson, M, Okabe, S, Ueda, H, Ariga, S, Nagasawa   +8 more
openaire   +2 more sources

Mast cells and complement system: Ancient interactions between components of innate immunity

Allergy, Volume 75, Issue 11, Page 2818-2828, November 2020., 2020
Abstract The emergence and evolution of the complement system and mast cells (MCs) can be traced back to sea urchins and the ascidian Styela plicata, respectively. Acting as a cascade of enzymatic reactions, complement is activated through the classical (CP), the alternative (AP), and the lectin pathway (LP) based on the recognized molecules.
Daniel Elieh Ali Komi, Farzaneh Shafaghat, Petri T. Kovanen, Seppo Meri   +3 more
wiley   +1 more source

Human complement factor H [PDF]

, 1986
We isolated cDNA clones coding for the functionally important tryptic N-terminal38- kDa fragment of human complement control protein factor H using polyclonal and monoclonal antibodies to screen a human liver cDNA library cloned in a bacterial expression
Dierich, Manfred P., Schulz, T. F., Schwaeble, W., Stanley, K. K., Weiss, Elisabeth H.   +4 more
core   +1 more source

Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis

International Brazilian Journal of Urology, 2012
Purpose To investigate the use of ClinProt technique to identify cancer markers in plasma of patients suffering from squamous cell carcinoma of the penis (SCCP). Materials and Methods Plasma of 36 healthy subjects and 25 patients with
Paulo Ornellas, Antonio Augusto Ornellas, Clizia Chinello, Erica Gianazza, Veronica Mainini, Marta Cazzaniga, Denise Abreu Pereira, Vanessa Sandim, Ana Sheila Cypriano, Leandro Koifman, Paulo Cesar Barbosa da Silva, Gilda Alves, Fulvio Magni   +12 more
doaj   +1 more source

Complement C3 serum levels in anorexia nervosa: a potential biomarker for the severity of disease? [PDF]

, 2011
BackgroundAnorexia nervosa carries the highest mortality rate of any psychiatric disorder. Even the most critically ill anorexic patients may present with normal 'standard' laboratory values, underscoring the need for a new sensitive biomarker.
Flierl, Michael A, Gaudiani, Jennifer L, Long, Carlin S, Mehler, Philip S, Sabel, Allison L, Stahel, Philip F   +5 more
core   +2 more sources