Results 241 to 250 of about 110,790 (277)

A small-molecule inhibitor of C5 complement protein

Nature Chemical Biology, 2019
The complement pathway is an important part of the immune system, and uncontrolled activation is implicated in many diseases. The human complement component 5 protein (C5) is a validated drug target within the complement pathway, as an anti-C5 antibody (Soliris) is an approved therapy for paroxysmal nocturnal hemoglobinuria.
Keith Jendza, Mitsunori Kato, Michael Salcius, Honnappa Srinivas, Andrea De Erkenez, Anh Nguyen, Doug McLaughlin, Celine Be, Christian Wiesmann, Jason Murphy, Philippe Bolduc, Muneto Mogi, Jose Duca, Abdel Namil, Michael Capparelli, Veronique Darsigny, Erik Meredith, Ritesh Tichkule, Luciana Ferrara, Jessica Heyder, Fang Liu, Patricia A. Horton, Michael J. Romanowski, Markus Schirle, Nello Mainolfi, Karen Anderson, Gregory A. Michaud   +26 more
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Structure/function of C5 convertases of complement

International Immunopharmacology, 2001
C5 convertases are serine proteases that cleave both C3 and C5. Alternative pathway C3/C5 convertases formed with monomeric C3b (C3b,Bb) because of their weak interaction with C5 primarily cleave C3 thereby opsonizing the cell surface with C3b. In contrast, C3/C5 convertases formed with a high density of C3b/cell exhibit higher affinities for C5 as ...
N, Rawal, M K, Pangburn
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Purification of Human Complement Protein C5

2013
Complement C5 is cleaved by proteolysis in the terminal phase of complement activation generating the pro-inflammatory C5a and membrane attack complex nucleator C5b. Whereas purification of its paralogues C3 and C4 from plasma is relatively straightforward, C5 purification is more complicated due to the lower amounts present and overlaps with the much ...
Sottrup-Jensen, Lars, Andersen, Gregers Rom   +1 more
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Structure and function of complement C5 convertase enzymes

Biochemical Society Transactions, 2002
The multisubunit enzymes of the complement system that cleave C5 have many unusual properties, the most striking of which is that they acquire their specificity for C5 following cleavage of another substrate C3. C5 convertases are assemblies of two proteins C4b and C2a (classical or lectin pathways) or C3b and Bb (alternative pathway) and additional ...
M K, Pangburn, N, Rawal
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Magnetic bead based assays for complement component C5

Journal of Immunological Methods, 2017
Two novel magnetic agarose bead based assays have been developed to measure complement component C5 interaction with C3b and the Factor I Modules (FIMs) of C7. One innovation was to couple C3b onto the magnetic agarose bead using the alternative pathway C3 convertase, which resulted in a linkage of the ligand by a covalent ester bond.
Richard G. DiScipio, Ingrid U. Schraufstatter   +1 more
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Isolation of human complement factors C3, C5 and H

Journal of Immunological Methods, 1985
An improved method for simultaneous purification of complement factors C3, C5 and H from human plasma has been developed. Using an initial batch separation technique with QAE-Sephadex, followed by chromatography on SP-Sephadex and gel filtration in Sephadex G-200, 600 mg of highly pure C3 can be prepared from 1600 ml of plasma.
A, Lundwall, G, Eggertsen
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Accelerated Wound Healing with Topical Application of Complement C5

Plastic & Reconstructive Surgery, 2012
Delayed-healing traumatic, surgical, and chronic wounds can be detrimental to patients and the health care system. The authors set out to investigate the effects of complement C5, a naturally occurring chemotactic cytokine, on wounds.The authors examined the effects of complement C5 on the rat paired skin incision model.
Hani, Sinno, Meenakshi, Malhotra, Justyn, Lutfy, Barbara, Jardin, Sebastian, Winocour, Fadi, Brimo, Lorne, Beckman, Kevin, Watters, Anie, Philip, Bruce, Williams, Satya, Prakash   +10 more
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Genetic defect in secretion of complement C5 in mice

Nature, 1979
A genetic deficiency of the fifth (C5) component of complement1-3, a serum glycoprotein of molecular weight (MW) 220,000 (ref. 4), has been found in 39% of inbred strains of mice3. Sera of deficient mice lack detectable C5 activity and protein2,3. In addition deficient mice produce antibody to mouse C5 when injected with sera from C5 sufficient (normal)
Ooi, Y M, Colten, H R
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Derivation of RNA aptamer inhibitors of human complement C5

Immunopharmacology, 1999
Specific aptamer inhibitors of the human complement C5 component were produced by the SELEX methodology of directed evolution of nucleic acid ligands. The SELEX procedure started with a pool of random-sequence, 2'F-pyrimidine-modified nuclease-stabilized RNA, and after twelve rounds of iterative C5 binding and nucleic acid amplification an evolved RNA ...
G, Biesecker, L, Dihel, K, Enney, R A, Bendele   +3 more
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Complement Inhibitors Targeting C3, C4, and C5

2000
Activation of the complement system (Fig. 1) is the key to the development of normal inflammatory responses against foreign pathogens. During the course of this activation process a number of biological events are initiated, including generation of small peptides that induce local inflammatory responses, tagging of foreign pathogens with complement ...
Arvind Sahu, Dimitrios Morikis, John D. Lambris   +2 more
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