Results 31 to 40 of about 25,476 (265)

An antibody-based biomarker discovery method by mass spectrometry sequencing of complementarity determining regions. [PDF]

Anal Bioanal Chem, 2011
Autoantibodies are increasingly used as biomarkers in the detection of autoimmune disorders and cancer. Disease specific antibodies are generally detected by their binding to specific antigens.
Dekker LJ, Zeneyedpour L, Brouwer E, van Duijn MM, Sillevis Smitt PA, Luider TM.   +5 more
europepmc   +3 more sources

Antibody complementarity-determining regions (CDRs) can display differential antimicrobial, antiviral and antitumor activities. [PDF]

PLoS ONE, 2008
BACKGROUND: Complementarity-determining regions (CDRs) are immunoglobulin (Ig) hypervariable domains that determine specific antibody (Ab) binding. We have shown that synthetic CDR-related peptides and many decapeptides spanning the variable region of a ...
Luciano Polonelli, José Pontón, Natalia Elguezabal, María Dolores Moragues, Claudio Casoli, Elisabetta Pilotti, Paola Ronzi, Andrey S Dobroff, Elaine G Rodrigues, Maria A Juliano, Domenico Leonardo Maffei, Walter Magliani, Stefania Conti, Luiz R Travassos   +13 more
doaj   +1 more source

A Role of the Third Complementarity-determining Region in the Affinity Maturation of an Antibody [PDF]

Journal of Biological Chemistry, 2001
We recently found that there are two distinct antibody maturation pathways for the immune response of C57BL/6 mice to (4-hydroxy-3-nitrophenyl) acetyl and that a junctional amino acid introduced at a point far in advance of somatic hypermutation determined which pathway of affinity maturation was used.
K, Furukawa, H, Shirai, T, Azuma, H, Nakamura   +3 more
openaire   +2 more sources

Complementarity-determining region clustering may cause CAR-T cell dysfunction [PDF]

, 2023
Chimeric antigen receptor (CAR)-T cell therapy is rapidly advancing as cancer treatment, however, designing an optimal CAR remains challenging. A single-chain variable fragment (scFv) is generally used as CAR targeting moiety, wherein the complementarity-
Dimberg, Anna,, Anna Dimberg, Ramachandran, Mohanraj, Helena Persson, Ramachandran, Mohanraj,, Xu Zhu, Thelander, Josefin,, Magnus Essand, Saronio, Giulia, Yasmin Andersson, Dimberg, Anna, Tina Sarén, Zhu, Xu, Andersson, Yasmin,, Marika Nestor, Sarén, Tina,, Yu, Di,, Paula Marti Torrell, Giulia Saronio, Zhu, Xu,, Nestor, Marika, Nestor, Marika,, Camilla Hofström, Andersson, Yasmin, Mohanraj Ramachandran, Yu, Di, Saronio, Giulia,, Marti Torrell, Paula,, Hofström, Camilla, Persson, Helena, Josefin Thelander, Essand, Magnus, Sarén, Tina, Marti Torrell, Paula, Di Yu, Thelander, Josefin, Hofström, Camilla,, Persson, Helena,, Essand, Magnus,   +38 more
core   +1 more source

Generation of humanized anti-DNA hydrolyzing catalytic antibodies by complementarity determining region grafting. [PDF]

, 2009
Humanization of nonhuman antibodies (Abs) has been carried out mainly for Abs which bind to antigen without catalytic activity. Here we report humanization of mouse-originated 3D8 (m3D8) mAbs (scFv, VH, and VL) with DNA hydrolyzing catalytic activity by ...
Kwon, MH, Dong-Sik Kim, Suk-Chan Lee, Lee, SH, Kim, DS, Lee, SC, Kim, JS, Kim, YS, Seung-Hyun Lee, Myung-Hee Kwon, Jeong-Sun Kim, Yong-Sung Kim   +11 more
core   +1 more source

Importance of Hypervariable Region 2 for Stability and Affinity of a Shark Single-Domain Antibody Specific for Ebola Virus Nucleoprotein. [PDF]

PLoS ONE, 2016
Single-domain antibodies derived from the unique New Antigen Receptor found in sharks have numerous potential applications, ranging from diagnostic reagents to therapeutics.
George P Anderson, Daniel D Teichler, Dan Zabetakis, Lisa C Shriver-Lake, Jinny L Liu, Stephen G Lonsdale, Sarah A Goodchild, Ellen R Goldman   +7 more
doaj   +1 more source

Phage-displayed antibody libraries of synthetic heavy chain complementarity determining regions [PDF]

, 2004
A structure-based approach was used to design libraries of synthetic heavy chain complementarity determining regions (CDRs). The CDR libraries were displayed as either monovalent or bivalent single-chain variable fragments (scFvs) with a single heavy ...
Frederic A. Fellouse, Sachdev S. Sidhu, Bing Li, Yvonne Chen, Germaine Fuh, Charles Eigenbrot   +5 more
core   +1 more source

HDX-MS study on garadacimab binding to activated FXII reveals potential binding interfaces through differential solvent exposure

mAbs, 2023
Hageman factor (FXII) is an essential component in the intrinsic coagulation cascade and a therapeutic target for the prophylactic treatment of hereditary angioedema (HAE).
Saw Yen Ow, Eugene A. Kapp, Vesna Tomasetig, Anton Zalewski, Jason Simmonds, Con Panousis, Michael J. Wilson, Andrew D. Nash, Matthias Pelzing   +8 more
doaj   +1 more source

Functional Analysis of T Cells Lacking Germline-Encoded Complementarity-Determining Regions [PDF]

, 2013
The diversity of antigen receptors and the specificity it underlies are the hallmarks of the cellular arm of the adaptive immune system. T and B lymphocytes are indeed truly unique in their ability to generate receptors capable of recognising virtually ...
Attaf, Meriem
core   +1 more source

Humanization of Antibodies using a Statistical Inference Approach

Scientific Reports, 2018
Antibody humanization is a key step in the preclinical phase of the development of therapeutic antibodies, originally developed and tested in non-human models (most typically, in mouse). The standard technique of Complementarity-Determining Regions (CDR)
Alejandro Clavero-Álvarez, Tomas Di Mambro, Sergio Perez-Gaviro, Mauro Magnani, Pierpaolo Bruscolini   +4 more
doaj   +1 more source