Results 251 to 260 of about 806,056 (297)
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Blood, 2002
AbstractWith the goal of creating a resource for in-depth study of myelopoiesis, we have executed a 2-pronged strategy to obtain a complementary DNA (cDNA) clone set enriched in hematopoietic genes. One aspect is a library subtraction to enrich for underrepresented transcripts present at early stages of hematopoiesis.
Xianyong, Ma +10 more
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AbstractWith the goal of creating a resource for in-depth study of myelopoiesis, we have executed a 2-pronged strategy to obtain a complementary DNA (cDNA) clone set enriched in hematopoietic genes. One aspect is a library subtraction to enrich for underrepresented transcripts present at early stages of hematopoiesis.
Xianyong, Ma +10 more
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“DNA-Dressed NAnopore” for complementary sequence detection
Biosensors and Bioelectronics, 2011Single molecule electrical sensing with nanopores is a rapidly developing field with potential revolutionary effects on bioanalytics and diagnostics. The recent success of this technology is in the simplicity of its working principle, which exploits the conductance modulations induced by the electrophoretic translocation of molecules through a ...
MUSSI, VALENTINA +6 more
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Patents on Random Complementary DNA Fragments?
Science, 1992The proposal by the National Institutes of Health (NIH) to patent products resulting merely from sequencing the human genome is a mistake: at worst, it is wrong in patent law; at best, it relies on deficiencies in law concerning what is "useful" as a requirement for patents.
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The binding of polyribonucleotides to the complementary strands of mitochondrial DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972Abstract We have purified the complementary strands (H and L) of rat mtDNA by equilibrium centrifugation in alkaline CsCl and studied their interaction with polyribonucleotides in neutral CsCl. H, the strand with the higher density in alkaline CsCl, was shifted to a higher density by poly(C) (20 mg/cm3), slightly shifted by alkali-degraded poly(U) (7
P, Borst, G J, Ruttenberg
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Nucleotide sequence of a complementary DNA for human ST2
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1992Human ST2 cDNA, a homologue of murine ST2 that is only expressed in growth-stimulated BALB/c-3T3 cells and a member of the primary response gene family induced by growth factors, was isolated from the cDNA library of an activated human helper T cell line, 5C10. Human ST2 has 67.6% identity in a 327 amino acid overlap to murine ST2.
S, Tominaga +5 more
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Human Lipoprotein Lipase Complementary DNA Sequence
Science, 1987Lipoprotein lipase is a key enzyme of lipid metabolism that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins. It has been proposed that the enzyme plays a role in the development of obesity and atherosclerosis.
K L, Wion +4 more
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Gene specific priming of complementary DNA synthesis
Molecular Biology Reports, 1976DNA, complementary to chicken globin mRNA was synthesized using either Avian Myeloblastosis virus reverse transcriptase, or E. coli DNA polymerase I. Transcriptase cDNA sediments at 9 S on sucrose gradients, and is 620 nucleotides in length, representing a complete copy of globin mRNA template. In contrast, Polymerase I cDNA sediments at 4 S, is 100 to
R J, Crawford, J R, Wells
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Nucleotide Sequence of Human Preproinsulin Complementary DNA
Science, 1980Recombinant bacterial plasmids that contain DNA complementary to human preproinsulin messenger RNA have been constructed. One clone contains the entire preproinsulin coding region, as well as the 3′ untranslated region of the messenger RNA and eight nucleotides of the 5′ untranslated region.
I, Sures +3 more
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CLONING OF DNA COMPLEMENTARY TO BOVINE PROLACTIN mRNA
Endocrinology, 1980We have cloned DNA complementary to mRNA coding for bovine prolactin (bPrl). Double-stranded cDNA prepared from bovine pituitary mRNA was inserted into the Pst I site of plasmid bPR322 by the dC x dG tailing technique and amplified in E. coli chi 1776. A recombinant plasmid containing bPrl cDNa was identified by hybridization to cloned rat Prl cDNA. It
W L, Miller, J P, Thirion, J A, Martial
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Nonrandom DNA sequencing of exonuclease III-deleted complementary DNA
Analytical Biochemistry, 1985The nonrandom DNA sequence analysis procedure of Poncz et al. [Proc. Natl. Acad. Sci. USA 79, 4298-4302 (1982)] was extensively modified to permit the determination of complementary DNA (cDNA) sequences containing G-C homopolymer regions. The recombinant cDNA plasmid was cleaved at a unique restriction enzyme site close to the cDNA and treated with ...
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