Results 41 to 50 of about 311,139 (302)

SpyTag/SpyCatcher display of influenza M2e peptide on norovirus-like particle provides stronger immunization than direct genetic fusion

Frontiers in Cellular and Infection Microbiology, 2023
IntroductionVirus-like particles (VLPs) are similar in size and shape to their respective viruses, but free of viral genetic material. This makes VLP-based vaccines incapable of causing infection, but still effective in mounting immune responses.
Vili Lampinen, Vili Lampinen, Stina Gröhn, Saana Soppela, Vesna Blazevic, Vesa P. Hytönen, Vesa P. Hytönen, Minna M. Hankaniemi   +7 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Transfer of the Integrative and Conjugative Element ICESt3 of Streptococcus thermophilus in Physiological Conditions Mimicking the Human Digestive Ecosystem

Microbiology Spectrum, 2023
Metagenome analyses of the human microbiome suggest that horizontal gene transfer (HGT) is frequent in these rich and complex microbial communities. However, so far, only a few HGT studies have been conducted in vivo.
Pauline Herviou, Aurélie Balvay, Deborah Bellet, Sophie Bobet, Claire Maudet, Johan Staub, Monique Alric, Nathalie Leblond-Bourget, Christine Delorme, Sylvie Rabot, Sylvain Denis, Sophie Payot   +11 more
doaj   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Antibiotic Resistance, Virulence Factors, Phenotyping, and Genotyping of E. coli Isolated from the Feces of Healthy Subjects

Microorganisms, 2019
Escherichia coli may innocuously colonize the intestine of healthy subjects or may instigate infections in the gut or in other districts. This study investigated intestinal E. coli isolated from 20 healthy adults.
Stefano Raimondi, Lucia Righini, Francesco Candeliere, Eliana Musmeci, Francesca Bonvicini, Giovanna Gentilomi, Marjanca Starčič Erjavec, Alberto Amaretti, Maddalena Rossi   +8 more
doaj   +1 more source

Evolution of Virulence, Fitness, and Carbapenem Resistance Transmission in ST23 Hypervirulent Klebsiella pneumoniae with the Capsular Polysaccharide Synthesis Gene wcaJ Inserted via Insertion Sequence Elements

Microbiology Spectrum, 2022
Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) is recognized as a threat worldwide, but the mechanisms underlying its emergence remain unclear. As most CR-hvKP isolates are not hypermucoviscous, we speculated that the evolution of the
Shuyi Wang, Qi Ding, Yawei Zhang, Anru Zhang, Qi Wang, Ruobing Wang, Xiaojuan Wang, Longyang Jin, Shuai Ma, Hui Wang   +9 more
doaj   +1 more source

Crystal structure of UBA2ufd-Ubc9: insights into E1-E2 interactions in sumo pathways [PDF]

, 2010
Canonical ubiquitin-like proteins (UBLs) such as ubiquitin, Sumo, NEDD8, and ISG15 are ligated to targets by E1-E2-E3 multienzyme cascades. The Sumo cascade, conserved among all eukaryotes, regulates numerous biological processes including protein ...
Miller, D.J., Asad M Taherbhoy, Schulman, B.A., Darcie J Miller, Steven N Seyedin, Huang Danny T., Wang, J., Kobe, B., Steven N. Seyedin, Seyedin Steven N., David W. Miller, Miller David W., Darcie J. Miller, Hunt Harold W., Miller, D.W., Taherbhoy, A.M., Danny T. Huang, Brenda A. Schulman, Miller Darcie J., Danny T Huang, Wang Jing, Harold W. Hunt, Harold W Hunt, Asad M. Taherbhoy, Schulman Brenda A., Jing Wang, Huang, D.T., Taherbhoy Asad M., David W Miller, Hunt, H.W., Seyedin, S.N., Brenda A Schulman   +31 more
core   +2 more sources

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Dissemination of antimicrobial resistance in microbial ecosystems through horizontal gene transfer

Frontiers in Microbiology, 2016
The emergence and spread of antibiotic resistance among pathogenic bacteria has been a rising problem for public health in recent decades. It is becoming increasingly recognized that not only antibiotic resistance genes (ARGs) encountered in clinical ...
Christian Johannes Hendrik Von Wintersdorff, John ePenders, John ePenders, Julius M van Niekerk, Nathan Dominic Mills, Snehali eMajumder, Lieke B van Alphen, Paul HM Savelkoul, Paul HM Savelkoul, Paul HM Savelkoul, Petra FG Wolffs, Petra FG Wolffs   +11 more
doaj   +1 more source