Results 211 to 220 of about 588,087 (304)

OsGSK2‐OsTCP19 Module Integrates Nitrogen and Brassinosteroid Signaling to Regulate Nitrogen Utilization and Root Growth in Rice

open access: yesAdvanced Science, EarlyView.
OsGSK2‐OsTCP19 module regulating nitrate response and lateral root (LR) development. Low‐nitrate condition results in reduced BR response, accumulation of OsGSK2 and phosphorylated OsTCP19, which suppresses the expression of nitrate‐responsive genes and LR‐development genes and impairs rice growth.
Yongqiang Liu   +11 more
wiley   +1 more source

Directed Evolution Improves the Catalytic Efficiency of APEX2‐Mediated Proximity‐Dependent RNA Labeling

open access: yesAdvanced Science, EarlyView.
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley   +1 more source

Engineering a Virus‐Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis

open access: yesAdvanced Science, EarlyView.
In this study, a Faustovirus‐derived polymerase (FvPolX) is engineered based on structural and sequence analysis of PolX enzymes. The optimal mutants demonstrate substantially enhanced catalytic activity toward dNTPs and 3′‐ONH2‐dNTPs compared to the wild‐type enzyme.
Chengjie Zhang, Lei Du, Shengying Li
wiley   +1 more source

Enzymatic Basis for the Oxidative Branch of Aromatic Amino Acid Fermentation Leading to p‐cresol Formation

open access: yesAdvanced Science, EarlyView.
In anaerobic bacteria, tyrosine serves as an electron donor in the oxidative Stickland fermentation branch, generating p‐hydroxyphenylacetate, ATP and reduced ferredoxin for decarboxylation into p‐cresol. ABSTRACT The phenolic metabolite p‐cresol is a byproduct of tyrosine fermentation by certain strictly anaerobic bacteria, including the human gut ...
Li Jiang   +15 more
wiley   +1 more source

Chiral‐Encoded Pt‐Ir Surfaces as Apparent Spin Filter for Enhanced Oxygen Reduction

open access: yesAdvanced Science, EarlyView.
The Chiral‐Induced Spin Selectivity (CISS) effect is employed in chiral‐encoded mesoporous Platinum‐Iridium (Pt‐Ir) electrocatalysts to enhance the oxygen reduction reaction (ORR). A break in spin symmetry leads to a substantial antagonistic difference in activity (>200%) when comparing two enantiomorphs of the same electrode.
Zikkawas Pasom   +5 more
wiley   +1 more source

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