Results 121 to 130 of about 3,251,371 (302)

A new RT-PCR assay for the revealing of Newcastle disease viruses by designing a pair of universal primers [PDF]

Iraqi Journal of Veterinary Sciences
Newcastle disease viruses (NDVs) possess a single-stranded, non-segmented RNA and are classified into different strains based on their level of pathogenicity.
Firas T. Al-Mubarak, Afnan G. Yaqoub, Manar M. Alnassar   +2 more
doaj   +1 more source

CHST1 Drives Immunotherapy Resistance in Triple‐Negative Breast Cancer by Orchestrating an Immunosuppressive Microenvironment via the NKRF–CCL20–Macrophage Axis

Advanced Science, EarlyView.
Proposed model of CHST1‐associated immune remodeling in triple‐negative breast cancer. In CHST1‐low tumors, greater nuclear accumulation of NKRF is associated with repression of an NF‐κB‐related CCL20 transcriptional program and an immune‐inflamed microenvironment.
Shu‐Hao Jiang, Xiao‐Han Ying, Ruo‐Qing Wang, Qun Zhang, Li Chen, Zhi‐Ming Shao, Jun‐Jie Li   +6 more
wiley   +1 more source

Genetic Characterization of Canine Enteric Coronaviruses (CECOV-IIa) From Dogs in Mosul City [PDF]

Iraqi Journal of Veterinary Sciences
Canine enteric coronavirus (CCoV) causes significant gastrointestinal illness in dogs, especially puppies, leading to high morbidity and mortality. This study investigated the presence, genetic characteristics, and clinical impact of enteric canine ...
Marwa Hamash, Safwan Al-Baroodi
doaj   +1 more source

The detection rate of PCV2 and/or TGEV infected preclinical samples using conventional PCR and RT-PCR, single MMPs-based UNDP-PCR and duplex MMPs-based UNDP-PCR methods.

, 2015
The detection rate of PCV2 and/or TGEV infected preclinical samples using conventional PCR and RT-PCR, single MMPs-based UNDP-PCR and duplex MMPs-based UNDP-PCR methods.
Xiujuan Zhang (174319), Xiaomin Zhao (566041), Lingling Chang (350993), Na Xing (823501), Qian Du (280345), Dewen Tong (93139), Yong Huang (15745), Zengguo Wang (823502)   +7 more
core   +1 more source

A Parylene Real Time PCR Microdevice [PDF]

, 2010
The polymerase chain reaction (PCR) is a powerful biochemical assay that is used in virtually all biochemical labs. By specifically amplifying a small sample of DNA, this technique is useful in the fields of paternity testing, forensics, and virus ...
Quach, Quoc Chan (Brandon)
core   +1 more source

Matrix Stiffness Orchestrates Mesenchymal Stem Cell Lineage Commitment Toward Osteogenesis and Adipogenesis Through the PIEZO1/SP1/STC2 Axis

Advanced Science, EarlyView.
Matrix stiffness directs mesenchymal stem cell lineage commitment through PIEZO1‐dependent activation of the SP1/STC2 pathway. Stiff matrices favor osteogenesis and suppress adipogenesis, whereas the loss of PIEZO1 function impairs early bone formation. STC2 retains lineage‐regulatory activity even when PIEZO1 is inactive.
Shuo Zhang, Siteng Li, Wenzhong Chen, Qingcheng Song, Haiyue Zhao, Peng Wang, Yiran Zhang, Wenquan Liang, Shaowei Zheng, Juan Wang, Wei Chen, Yanbin Zhu, Yingze Zhang   +12 more
wiley   +1 more source

Detecção de rotavírus em amostras de águas de superfície através de técnicas moleculares e de cultivo celular [PDF]

, 2008
TCC(graduação) - Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Biologia.Atualmente a contaminação do ambiente aquático e conseqüentemente dos recursos hídricos, vêm tomando importância frente à preocupação com a escassez da água.
Moresco, Vanessa
core  

Application of Conventional Reverse Transcription (RT) Polymerase Chain Reaction (RT-PCR) for Detection of African Horse Sickness Virus Serogroup

, 2023
A conventional reverse transcriptase (RT) polymerase chain reaction (RT-PCR) for detection of African Horse sickness virus serogroup in cell culture was developed and evaluated.
Karamalla, Siham T., Eldegail, Mawahib H., Gubran, Ahmed A., Elmalik , Khitma H., Aradaib, Imadeldin E.   +4 more
core  

AS‐TLC‐Based High‐Throughput Screening Integrated with CuAAC Optimization Strategy Facilitates the Rapid Discovery of YTHDC1 Inhibitor

Advanced Science, EarlyView.
We developed Affinity Selection Thin‐Layer Chromatography (AS‐TLC) as a method for high‐throughput screening (HTS) of compound mixtures. Furthermore, we integrated activity assays with AS‐TLC and identified a YTH domain‐containing protein 1 (YTHDC1) inhibitor. Building on this hit, we applied copper(I)‐catalyzed azide–alkyne cycloaddition (CuAAC) click
Mingchen Wang, Shuiping Fu, Ye Xu, Jia Gao, Jiwei Ren, Xu Yang, Hesong Xu, Ge Sun, Yanjie Li, Yanlin Wang, Lu Jin, Peijuan Chen, Ying Xu, Jianhao Li, Hua Lin, Chujiao Hu, Kaixian Chen, Huan Xiong, Cheng Luo, Shijie Chen   +19 more
wiley   +1 more source

Determinación de Salmonella spp. por PCR en tiempo real y método convencional en canales de bovinos y en alimentos de la vía pública de Montería, Córdoba Detection of Salmonella spp. by real time PCR and standard methods in cattle carcasses and public fast food outlets in Montería, Córdoba

Infectio, 2008
Objetivo. Detectar Salmonella spp. por PCR en tiempo real (PCR-TR) y el método convencional en alimentos de la vía pública y canales de bovino de una planta certificada de beneficio animal con sistema HACCP de Montería. Materiales y métodos.
Edna Yánez, Salim Máttar, Alba Durango
doaj