Results 141 to 150 of about 3,251,371 (302)

Molecular Amplification and Cell Culturing Efficiency for Enteroviruses’ Detection in Cerebrospinal Fluids of Algerian Patients Suffering from Meningitis

Viruses
Enteroviruses (EVs) represent a major cause of viral meningitis, being responsible for nearly 1 billion infections each year worldwide. Several techniques were developed to obtain better diagnostic results of EV infections.
Abdelwahab Rai, Zohra Ammi, Dahbia Leila Anes-Boulahbal, Aymen Amin Assadi, Abdeltif Amrane, Oussama Baaloudj, Lotfi Mouni   +6 more
doaj   +1 more source

Natural Product Toosendanin Suppresses the Malignant Development of Skin Melanoma by Targeting BNC2 for Degradation

Advanced Science, EarlyView.
BNC2 exhibits context‐dependent opposing functions across multiple cancer types. This study reveals BNC2 as an oncogenic driver of melanoma proliferation and metastasis through transcriptional activation of PIK3CA. The natural compound TSN simultaneously degrades BNC2 and its oncogenic partner SMAD3 via CRBN‐dependent ubiquitination.
Hui Dai, Xin Mei Zhang, Jing Cheng Zhang, Xu Ting Deng, Qiao Liu, Mustafa Hussein Ali, Fang Zhou Yin, Wu Yin   +7 more
wiley   +1 more source

RT-PCR primers used for CaMKII isoform analysis.

, 2016
RT-PCR primers used for CaMKII isoform analysis.
Shih-Chin Hsu (3358892), Yi-Hung Chen (1506184), Ming-Jiun Yu (715224), Kuan-Ying Lee (3358889)   +3 more
core   +1 more source

Multi‐Technique Integration Identifies O‐GlcNAc Transferase in Fibroblast‐Like Synoviocytes as a Therapeutic Target for Rheumatoid Arthritis

Advanced Science, EarlyView.
AI‑guided single‑cell transcriptomics identifies OGT as a key regulator enriched in rheumatoid arthritis fibroblast‑like synoviocytes (RA‐FLSs). Inflammatory stimuli activate the OGT–SAP130 axis, which modulates the H3K27ac/H3K27me3 balance and epigenetically silences BTG2, promoting aggressive transformation of RA‑FLSs.
Duoli Xie, Jie Huang, Chunhao Cao, Zhenchao Tang, Xingchen Li, Jianmin Guo, Xinxin Chen, Fang Wang, Dongyi He, Jie Li, Yu Du, Bing He, Jianhua Yao, Aiping Lu, Chao Liang   +14 more
wiley   +1 more source

RT-PCR and qPCR primers of examined lncRNA genes.

, 2017
RT-PCR and qPCR primers of examined lncRNA genes.
Jianbo Fu (3836437), Fengxin Gao (3836434), Xiaoning Zhang (369283), Peng Zhang (2071), Yuqing Wang (110531), Xuhui Zeng (169847)   +5 more
core   +1 more source

Carbohydrate‐Mediated Cellular Uptake of Boronic Acid Hybrids In Cellulo and Insulin‐Deficient Zebrafish: Live Imaging and Application of Multiphoton FLIM

Advanced Science, EarlyView.
Boronic acid‐based fluorescent hybrids have been used as part of a versatile platform for real‐time metabolic bioimaging across cellular and organismal scales. Including glucose‐responsive imaging in living cells and in an insulin‐deficient vertebrate model, this work opens new opportunities for the development of precision diagnostics, metabolic ...
Haobo Ge, John S. Fossey, David Gonzalez Calatayud, Stephen E. Flower, Sabrina Wang, Hanna Aitessaid, Melita Tvardauskaite, Julia Dudzic, Max Jewell, Charareh Pourzand, Stanley W. Botchway, David Gurevich, Tony D. James, Sofia I. Pascu   +13 more
wiley   +1 more source

Sequences of primers used for Q-RT-PCR amplification.

, 2016
Sequences of primers used for Q-RT-PCR amplification.
Yixing Feng (497346), Zhihao Jiao (2697175), Bing Shao (359353), Jiachen Shi (2441017), Zhaobin Zhang (246390), Pin Zhang (447389)   +5 more
core   +1 more source

A Dual‐Mode In Situ EV‐miRNA Profiling Platform for Machine‐Learning‐Assisted Gastric Cancer Liquid Biopsy

Advanced Science, EarlyView.
An in situ dual‐mode platform integrates fusogenic liposomes with localized autocatalytic DNA circuits for plasma‐derived extracellular vesicle microRNA analysis. Following membrane fusion, target miRNAs trigger feedback‐assisted LECHA amplification inside intact vesicles, generating fluorescence and electrochemical outputs. Machine learning integrates
Wenbin Li, Rui Fan, Kun Xie, Jiehua Zhong, Yitong Zhu, Tingting Ji, Yuanyuan Qin, Shijin Peng, Yu Zhang, Yuhang Guo, Tiange Zhang, Chunchen Liu, Bo Li, Lei Zheng, Ye Zhang, Xiaohui Yan   +15 more
wiley   +1 more source

Comparative Detection of H5N1 Avian Influenza Virus Using Conventional Rt-PCR and Real Time Rt-PCR

International Journal of Virology, 2012
N.M. Hagag, A. Arafa, M.A. Shalaby, A.A. El-Sanousi, M.M. Aly   +4 more
openaire   +1 more source

Human Parechovirus A conventional nested genotyping RT-PCR ["Harvala assay"; 2008-2015] v1

, 2017
I and my team used this assaybetween 2008-2015; we dubbed it the "Harvala assay". It produces an amplicon that includes the 3' end of VP3 and the 5' end of VP1, spanning the junction. In silicosequence alignments indicated the highly degenerate olignucleotides could theoretically detect af least HPeV 1-7, 17 and 18.
openaire   +1 more source