Results 151 to 160 of about 3,251,371 (302)

RT-PCR primers used to amplify PA biosynthetic genes.

open access: yes, 2019
RT-PCR primers used to amplify PA biosynthetic genes.
D. S. Kishor (6458960)   +9 more
core   +1 more source

Oligosaccharides With Defined Glycosidic Bonds Shape Gut Microbial Succession and Metabolism Via Bond‐Specific Microbial Responders

open access: yesAdvanced Science, EarlyView.
Oligosaccharides with defined glycosidic bonds drive divergent gut microbial succession and shape unique metabolic profiles. Absolute time‐series profiling with Bayesian generalized Lotka–Volterra modeling identifies bond‐specific microbial responders, while monoculture validation and metaproteomics reveal linkage‐matched transport and cleavage modules.
Xiaoxuan Lu   +10 more
wiley   +1 more source

Development and Clinical Application of a Molecular Assay for Four Common Porcine Enteroviruses

open access: yesVeterinary Sciences
Porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), and porcine rotavirus-A (PoRVA) are the four main pathogens that cause viral diarrhea in pigs, and they often occur in mixed ...
Zhonghao Xin   +11 more
doaj   +1 more source

RT-PCR primers for GPCRs and signaling components.

open access: yes, 2018
RT-PCR primers for GPCRs and signaling components.
Shobha Mummalaneni (565983)   +6 more
core   +1 more source

YTHDC1 Orchestrates Glucose and Glutamate Rewiring to Overcome Lethal Metabolic Stress in Triple‐Negative Breast Cancer

open access: yesAdvanced Science, EarlyView.
Lai et al. find that m6A reader YTHDC1 is specifically overexpressed in triple‐negative breast cancer (TNBC) and orchestrates glucose and glutamine metabolism in an m6A‐dependent manner to confer robust adaptability to lethal metabolic stress. Leveraging YTHDC1 knockdown‐induced metabolic vulnerability, an ATF4 mRNA‐targeted nanotherapy is developed to
Zheng‐Hao Lai   +12 more
wiley   +1 more source

RT-PCR validation of selected genes from microarray data.

open access: yes, 2016
RT-PCR validation of selected genes from microarray data.
Christina Tam (2583046)   +7 more
core   +1 more source

Glucose and Glutamine Deprivation Promotes Breast Cancer Lung Metastasis via BRD4‐Dependent Enhancer Activation

open access: yesAdvanced Science, EarlyView.
To model a nutrient‐restricted tumor microenvironment, breast cancer cells were adapted to low glucose and glutamine levels. In the adapted cells, BRD4‐driven enhancer activation increases pro‐invasive EDN1 expression, facilitated by ATF3/c‐JUN and reinforced by enhancer RNA.
Poshan Yugal Bhattarai   +4 more
wiley   +1 more source

Human Parechovirus A conventional nested genotyping RT-PCR ["Harvala assay"; 2008-2015] v3

open access: yes, 2017
I and my team used this assaybetween 2008-2015; we dubbed it the "Harvala assay". It produces an amplicon that includes the 3' end of VP3 and the 5' end of VP1, spanning the junction. In silicosequence alignments indicated the highly degenerate olignucleotides could theoretically detect af least HPeV 1-7, 17 and 18.
openaire   +1 more source

An Injectable ROS‐Responsive Nanozyme Hydrogel Regulates the Uterine Microenvironment to Prevent Intrauterine Adhesions

open access: yesAdvanced Science, EarlyView.
The injectable CeTA@GPP hydrogel is administered via intrauterine injection, forming a protective barrier. In the high ROS inflammatory microenvironment of injured endometrium, boronate ester cleavage triggers responsive CeTA release and local enrichment.
Peixian Cheng   +8 more
wiley   +1 more source

Human Parechovirus A conventional nested genotyping RT-PCR ["Harvala assay"; 2008-2015] v2

open access: yes, 2017
I and my team used this assaybetween 2008-2015; we dubbed it the "Harvala assay". It produces an amplicon that includes the 3' end of VP3 and the 5' end of VP1, spanning the junction. In silicosequence alignments indicated the highly degenerate olignucleotides could theoretically detect af least HPeV 1-7, 17 and 18.
openaire   +1 more source

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