Results 271 to 280 of about 3,251,371 (302)

Detection of Respiratory Syncytial Virus in Suspected Asthmatic Children by Conventional RT-PCR Assays in Kathmandu

Journal of Nepal Biotechnology Association, 2023
Respiratory Syncytial Virus (RSV) is the most common causative agent for lower respiratory tract infections in children particularly in infants. At least 50% of children are infected with RSV by the age of 2 to 3 years of age. However, the diagnosis of the RSV infection and its association with risk factors for asthma in asthmatic children is not ...
Stuti Khadka, Anil Kumar Sah, Jeena Amatya   +2 more
openaire   +1 more source

The application of conventional cytogenetics, FISH, and RT-PCR to detect genetic changes in 70 children with ALL

Annals of Hematology, 2008
We investigated bone marrow cells of 70 acute lymphoblastic leukemia children by conventional cytogenetics (CC), fluorescence in situ hybridization (FISH), and reverse transcription polymerase chain reaction (RT-PCR) methods. CC and RT-PCR for fusion genes BCR/ABL, MLL/AF4, E2A/PBX1, TEL/AML1 were performed at diagnosis in each patient.
Soszyńska, Krystyna, Mucha, Barbara, Dębski, Robert, Skonieczka, Katarzyna, Duszeńko, Ewa, Kołtan, Andrzej, Wysocki, Mariusz, Haus, Olga   +7 more
openaire   +2 more sources

Comparative Evaluation of Conventional RT-PCR with Chip Based Real Time RT- PCR (TrueNat) for Detection of COVID-19

International Journal of Health Sciences and Research
The outbreak of SARS-CoV-2 has required the creation of high-speed, sensitive, and convenient molecular diagnostic solutions. This paper compares the diagnostic capability of the chip-based real-time RT-PCR (TrueNat) with the traditional real-time RT-PCR in detection of COVID-19.
Kanishtha Sharma, Kuldeep Singh, Gazala Abbas   +2 more
openaire   +1 more source

Conventional RT-PCR for Yellow Fever Virus

The yellow fever virus (YFV), prototype of the genus Orthoflavivirus (family Flaviviridae), contains a positive-sense RNA genome that is translated as a single polyprotein which comprises approximately 11,000 nucleotides, encoding 3411 amino acids. In recent years, a number of molecular assays have been reported for YFV RNA detection, with different ...
Nieli Rodrigues, da Costa Faria, Renata, Monteiro-Maia, Priscila Conrado Guerra, Nunes, Monique, da Rocha Queiroz Lima, Fernanda, de Bruycker-Nogueira   +4 more
openaire   +2 more sources

Advancing broad bean true mosaic virus detection using conventional RT-PCR and real-time RT-PCR with novel primer set design

Journal of Virological Methods
Broad bean true mosaic virus (BBTMV) infects broad beans and peas, reducing yield. As BBTMV is transmitted through broad beans, many countries have implemented regulations to prevent the distribution of infected seeds. Currently, enzyme-linked immunosorbent assay (ELISA) is commonly used to detect BBTMV. While the PCR-based method is preferred for seed
Fumino Nito, Hitoshi Oya, Takayuki Matsuura, Hironobu Yanagisawa   +3 more
openaire   +2 more sources

Plasma collected from heparinized blood is not suitable for HCV-RNA detection by conventional RT-PCR assay

Journal of Virological Methods, 1993
Detection of hepatitis C virus-RNA (HCV-RNA) in serum or in plasma is considered a reliable marker for ongoing HCV infection. HCV-RNA was measured routinely in plasma collected from heparinized blood of patients with chronic hepatitis C and varying results were found. In this study, HCV-RNA was detected in peripheral blood mononuclear cells (PBMCs) and
M, Willems, H, Moshage, F, Nevens, J, Fevery, S H, Yap   +4 more
openaire   +2 more sources

Development of real-time and conventional RT-PCR assays for the detection of potato yellow vein virus (PYVV)

Journal of Virological Methods, 2006
Potato yellow vein virus (PYVV) is considered a quarantine pathogen in the European and Mediterranean Plant Protection Organization (EPPO) area. This virus is widespread and damaging at its centre of origin in South America. Current detection methods are either time-consuming or difficult to interpret. This paper reports the development of a sensitive,
R, López, C, Asensio, M M, Guzman, N, Boonham   +3 more
openaire   +2 more sources

Comparison of Conventional RT-PCR and Real-time RT-PCR Assays for Diagnosis of Grapevine fanleaf nepovirus (GFLV)*

2016
Recently advances in biotechnology and molecularbiology have played a significant role in development of serological andmolecular assays for detection of plant viruses. However, specificity andsensitivity of them may show variation according to pathogens and hosts. Inthis study, Grapevine fanleaf nepovirus(GFLV) which was the most prevalent viral agent
ÖNDER, Serkan, PAYLAN, İsmail Can, GÜMÜŞ, Mustafa   +2 more
openaire   +1 more source

Detection of PAX8–PPARG fusion transcripts in archival thyroid carcinoma samples by conventional RT‐PCR

Genes, Chromosomes and Cancer, 2011
AbstractThe t(2;3)(q13;p25) occurs in a subgroup of follicular‐patterned thyroid tumors and leads to a fusion of the genes encoding for the thyroid‐specific transcription factor paired box 8 (PAX8) and the peroxisome proliferator‐activated receptor gamma (PPARγ).
Markus, Klemke, Norbert, Drieschner, Gazanfer, Belge, Käte, Burchardt, Klaus, Junker, Jörn, Bullerdiek   +5 more
openaire   +2 more sources

Detection of Grapevine leafroll-associated virus 7 using real time qRT-PCR and conventional RT-PCR

Journal of Virological Methods, 2012
Nine isolates of Grapevine leafroll-associated virus 7 (GLRaV-7) from diverse geographical regions were sequenced to design more sensitive molecular diagnostic tools. The coat protein (CP) and heat shock protein 70 homologue (HSP70h) genes of these nine isolates were sequenced.
Al Rwahnih M, Osman F, Sudarshana M, Uyemoto J, Minafra A, Saldarelli P, Martelli G, Rowhani A   +7 more
openaire   +4 more sources