Results 81 to 90 of about 3,251,371 (302)

Artemisinin‐Loaded Core‐Shell Microspheres Modulate Histone Lactylation to Remodel the Microenvironment for Cartilage Protection in Rheumatoid Arthritis

Advanced Healthcare Materials, EarlyView.
ART@PLGA@GH‐A microspheres are developed via microfluidic technology for intra‐articular delivery of artemisinin. The system remodels rheumatoid arthritis microenvironments by regulating metabolic and inflammatory disorders, reducing lactate‐driven histone lactylation and cellular senescence, while restoring cartilage homeostasis and protecting joint ...
Shijie Meng, Jianxin Li, Yuyang Luo, Yisi Liu, Feng Xing, Yingchen Ni, Fengyuan Zhang, Zhicheng Fan, Hua Xu, Weidong Zhang, Youhua Wang   +10 more
wiley   +1 more source

Development of a One-Step SYBR Green I Real-Time RT-PCR Assay for the Detection and Quantitation of Araraquara and Rio Mamore Hantavirus

Viruses, 2013
Hantaviruses are members of the family Bunyaviridaeandare an emergingcause of diseaseworldwidewithhighlethalityin the Americas. In Brazil, thediagnosis forhantaviruses is basedonimmunologictechniquesassociatedwithconventionalRT-PCR.Anovelone ...
Luiz Tadeu Moraes Figueiredo, Michelly de Pádua, Aline Rafaela da Silva Rodrigues Machado, Alex Martins Machado, William Marciel de Souza   +4 more
doaj   +1 more source

Molecular Detection of a Novel Human Influenza (H1N1) of Pandemic Potential by Conventional and Real-Time Quantitative RT-PCR Assays [PDF]

Clinical Chemistry, 2009
Abstract Background: Influenza A viruses are medically important viral pathogens that cause significant mortality and morbidity throughout the world. The recent emergence of a novel human influenza A virus (H1N1) poses a serious health threat. Molecular tests for rapid detection of this virus are urgently needed.
Smith, GJ, Peiris, JSM, Yuen, KY, Guan, Y, Poon, LLM, Chan, KH, Leung, CSW   +6 more
openaire   +5 more sources

RT-PCR and MARK-B test results.

, 2021
(A) Peak current values of RT-PCR-positive and RT-PCR-negative samples. (B) Correlation between peak current values (MARK-B, log2 scale) and the Ct values of the RdRp gene (60 RT-PCR-positive samples; ○, MARK-B false negative).
Sung Jin Jo (2524933), Seungok Lee (6851639), Sang-hyun Shin (11534660), Jehoon Lee (9243233), Jungrok Kim (11534663)   +4 more
core   +1 more source

Microgels Enable iPSCs to Assemble, Expand, and Differentiate Into Organoids—From Sizable to High‐Throughput

Advanced Materials, EarlyView.
A chemically defined PEG‐based microgel platform enables scalable, reproducible production of three‐dimensional microgel‐iPSC constructs in multiple sizes. The system is compatible with high‐throughput automation and supports human iPSC expansion and differentiation within a single construct, including directed differentiation into cardiac organoids ...
Laura Klasen, Matthias Mork, Ramin Nasehi, Vasudha Turuvekere Krishnamurthy, Kira Zeevaert, Aaron Babendreyer, Jacopo Di Russo, Wolfgang Wagner, Laura De Laporte   +8 more
wiley   +1 more source

MicroRNA q-RT-PCR data.

, 2016
MicroRNA q-RT-PCR data.
Jun-Jie Wang (796628), Yong Zhao (84950), Wei Ge (39218), Xin-Qi Liu (2667751), Lan Li (73597), Zhi-Hui Hao (812682), Hong-Fu Zhang (313561), Qing-Yuan Sun (148635), Ling-Jiang Min (149121), Lian-Qin Zhu (2794867), Wei-Dong Zhang (673110), Jing-Cai Liu (2158765)   +11 more
core   +1 more source

Self‐Sealing Microneedles Improve Precision and Reduce Reflux in Subretinal Cell Transplantation

Advanced Materials Technologies, EarlyView.
Ultrafine 49G microneedles enable subretinal delivery of induced pluripotent stem cell‐derived retinal pigment epithelial cells through a 50 µm outer diameter tip. Compared with conventional 38G microcannulas, these microneedles reduce cell suspension reflux in a rabbit model, highlighting the potential of microinvasive delivery instruments to improve ...
Jared Ching, Hinako Ichikawa, Aya Motoyoshi, Shin Tanaka, Liam Morrow, Satoshi Shirae, Noriko Sakai, Shohei Kitahata, Michiko Mandai, Kazuaki Kadonosono   +9 more
wiley   +1 more source

Accuracy of subtype identification by RT-PCR (conventional and real-time).

, 2016
Accuracy of subtype identification by RT-PCR (conventional and real-time).
Jeannette Muñoz-Aguayo (3279126), Carol Cardona (206111), Susan Fleming (3279123), Erica Spackman (77138)   +3 more
core   +1 more source

Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies [PDF]

, 2009
De Gregoris TB, Borra M, Biffali E, et al. Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies. BMC Molecular Biology.
De Gregoris, Tristano Bacchetti, Clare, Anthony S., Biffali, Elio, Elio Biffali, Thomas Bekel, Bekel, Thomas, Richard R Kirby, Borra, Marco, J Grant Burgess, Marco Borra, Burgess, J. Grant, Bacchetti De Gregoris, Tristano, Anthony S Clare, Kirby, Richard R., Tristano Bacchetti De Gregoris   +14 more
core   +1 more source

Attomolar Detection of HIV‐1 With Label‐Free RCA‐rCRISPR on Smartphone

Advanced Science, EarlyView.
A new diagnostic strategy integrating rolling circle amplification (RCA) with a plasmid reporter–based ratiometric CRISPR (rCRISPR) system was developed to detect unfragmented HIV genomes with single‐digit attomolar sensitivity. The method quantifies targets using the plasmid relaxation ratio and employs a smartphone‐based mini‐gel device to enable ...
Noor Mohammad, Anastasiia Steksova, Yuyang Tang, Leyao Huang, Alireza Velayati, Shengwei Zhang, Aditi Dey Poonam, Sina Jamalzadegan, Matthew Breen, Hongjie Chen, Mahsa Bagi, Guochun Jiang, Qingshan Wei   +12 more
wiley   +1 more source