Results 61 to 70 of about 3,251,371 (302)

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Appling real time RT-PCR for bluetongue virus detection in Iran

Archives of Razi Institute, 2011
During 2009-10, real time RT-PCR and conventional RT-PCR techniques Used for detecting BTVs RNA in310 blood samples. For real time and gel based RT-PCR segment-1 and segment-10 selected as conservegenes to search any BTV strains. Using these methods, 58 (
Shoshtari, A., Jeirani, F.,, Mahravani, H.,, Azimi, S.M.   +3 more
doaj  

Genetic Analysis of Field Isolates of Infectious Bursal Disease Virus in Iraqi Farms

The Iraqi Journal of Veterinary Medicine, 2020
Sixty samples of bursa of Fabricius were collected from broiler chickens suspected to be infected with infectious bursal disease virus (IBDV) in different areas of Iraq for molecular evaluation.
Rawaa S. Jumaa, Aida B. Allawi, Rebah N. Jabbar   +2 more
doaj   +1 more source

Analytical sensitivity comparison of 14 conventional and three rapid RT-PCR assays for SARS-CoV-2 detection

Journal of Virological Methods, 2021
Recent reports have compared the analytical sensitivities of some SARS-CoV-2 RT-PCR assays, but differences in the viral materials used for these evaluations made comprehensive conclusions difficult. We carried out a direct comparison of the analytical sensitivities of 14 conventional and three rapid RT-PCR assays for the detection of SARS-CoV-2.
Wang, Xueliang, Huang, Zhongqiang, Song, Jian, Xiao, Yanqun, Wang, Hualiang   +4 more
openaire   +2 more sources

RT-LAMP and RT-PCR primer sequences.

, 2019
RT-LAMP and RT-PCR primer sequences.
Michele Tchokwansi (6635822), Léopold Lehman (6635843), Lawrence Ayong (309445), Emmanuel Elanga-Ndille (6635840), Umaru Banlanjo (6635825), Sylvie Kemleu (3337653), Balotin Fogang (6635831), Estelle Essangui (3337656), Carole Else Eboumbou Moukoko (6411722), Niels Nguedia (6635819), Christiane Donkeu (3337671), Marie Biabi (6635834), Glwadys Cheteug (6635837), Franklin Maloba (6635828)   +13 more
core   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Rapid and accurate diagnosis of Foot-and-mouth disease virus by Real-time PCR in field samples

Archives of Razi Institute, 2012
During 2010-2011, Real-time PCR procedure was used to detecting FMDV RNA on 147 epithelium samples from the field. In this survey, for Real-time PCR from 3D gene segment as conserve region selected for tracking all of seven serotypes FMDV.
Mahravani, H., Azimi, M., Ahmadi, A.,, Jeirani1, F., Jeirani1, F., Ahmadi, A.,, Azimi, M., Mahravani, H.   +7 more
doaj  

Droplet Digital PCR Enhances Sensitivity of Canine Distemper Virus Detection

Viruses
Canine distemper virus (CDV) poses a substantial threat to diverse carnivorans, leading to systemic and often fatal diseases. Accurate and prompt diagnosis is paramount for effective management and curbing further transmission.
Victoria Iribarnegaray, Guillermo Godiño, Camila Larrañaga, Kanji Yamasaki, José Manuel Verdes, Rodrigo Puentes   +5 more
doaj   +1 more source

A convenient rapid culture assay for the detection of enteroviruses in clinical samples: comparison with conventional cell culture and RT-PCR [PDF]

Journal of Medical Microbiology, 2008
A convenient rapid culture assay (RCA) for the detection of enteroviruses was evaluated against RT-PCR using 576 stool and 102 cerebrospinal fluid (CSF) samples. One hundred and ninety stool samples were also tested by conventional cell culture (CCC).
Elena, Terletskaia-Ladwig, Silvia, Meier, Ralph, Hahn, Michael, Leinmüller, Franz, Schneider, Martin, Enders   +5 more
openaire   +2 more sources

Quantitative-RT-PCR analysis.

, 2019
Quantitative-RT-PCR analysis.
Cléa Melenotte (6817877), Soraya Mezouar (6817880), Simon Benatti (6817883), Régis Costello (6817886), Jacques Chiaroni (473993), Guido Kroemer (82802), Jean-Louis Mege (18308), Christian Devaux (246026), Didier Raoult (3833), Amira Ben Amara (185263)   +9 more
core   +1 more source