Results 51 to 60 of about 3,251,371 (302)

Missed detections of influenza A(H1)pdm09 by real-time RT–PCR assay due to haemagglutinin sequence mutation, December 2017 to March 2018, northern Viet Nam

Western Pacific Surveillance and Response, 2019
Introduction: There are two methods of reverse transcription polymerase chain reaction (RT–PCR) that have been the common methods to detect influenza infections: conventional and real-time RT–PCR.
Hoang Vu Mai Phuong, Ung Thi Hong Trang, Nguyen Le Khanh Hang, Nguyen Thanh Thuy, Le Thi Thanh, Nguyen Vu Son, Nguyen Phuong Anh, Tran Thi Thu Huong, Vuong Duc Cuong, Le Quynh Mai   +9 more
doaj   +1 more source

Detection of lumpy skin disease virus in cattle using real-time polymerase chain reaction and serological diagnostic assays in different governorates in Egypt in 2017 [PDF]

Veterinary World, 2019
Background and Aim: Lumpy skin disease (LSD), is a highly infectious viral disease of cattle, caused by LSD virus (LSDV) which belongs to the genus Capripoxvirus of family Poxviridae. In the summer of 2017, skin lesions suggestive of LSD were observed in
Gamil Sayed Gamil Zeedan, Ayman Hamid Mahmoud, Abeer Mostafa Abdalhamed, Khaled Abd El-Hamid Abd El-Razik, Manal Hamdy Khafagi , Hala Abdoula Ahmed Abou Zeina   +5 more
doaj   +1 more source

Regulation of the lncRNA NEAT1 by p53‐ΔNp63 crosstalk modulates the DNA damage response and therapeutic efficacy in HNSCC

Molecular Oncology, EarlyView.
In head and neck squamous cell carcinoma (HNSCC) p53 and p63 exert opposite roles on the transcription regulation of the lncRNA NEAT1. Under basal conditions, p53 levels are low and p63 represses NEAT1 expression. Upon genotoxic stress, p53 is rapidly induced, displacing p63 from the NEAT1 promoter leading to NEAT1 transcriptional activation and ...
Sara De Domenico, Veronica La Banca, Silvia D'Amico, Stefano Scalera, Sara Nicolai, Angelo Peschiaroli   +5 more
wiley   +1 more source

Molecular characterization and high prevalence of Tritrichomonas foetus in bulls from the North-West Province of South Africa using real - time polymerase chain reaction (PCR) and conventional PCR diagnostics [PDF]

Veterinary World
Background and Aim: Bovine trichomonosis, caused by Tritrichomonas foetus, is a significant reproductive disease that impacts cattle productivity and breeding efficiency. In South Africa, routine diagnostic methods often depend on culture and microscopy,
Afaque H. Syed, Mpinda Edoaurd Tshipamba, Ngoma Lubanza, Baitsholetsi G. Mokolopi, Jean Marie Dibungi Luseba, Mulunda Mwanza   +5 more
doaj   +1 more source

Rapid differential diagnosis between extrapulmonary tuberculosis and focal complications of brucellosis using a multiplex real-time PCR assay. [PDF]

PLoS ONE, 2009
BACKGROUND: Arduous to differ clinically, extrapulmonary tuberculosis and focal complications of brucellosis remain important causes of morbidity and mortality in many countries. We developed and applied a multiplex real-time PCR assay (M RT-PCR) for the
María Isabel Queipo-Ortuño, Juan D Colmenero, Pilar Bermudez, María José Bravo, Pilar Morata   +4 more
doaj   +1 more source

Comparison of the detection rate of PEDV infected preclinical fecal samples by conventional RT-PCR, functionalized nanoparticles-based PCR and SYBR Green real-time RT-PCR assay.

, 2016
Comparison of the detection rate of PEDV infected preclinical fecal samples by conventional RT-PCR, functionalized nanoparticles-based PCR and SYBR Green real-time RT-PCR assay.
Xiaoxiao Guan (3390242), Xiujuan Zhang (174319), Xiaoya Wang (297374), Xiaomin Zhao (566041), Na Xing (823501), Qian Du (280345), Bin An (3390245), Dewen Tong (93139), Beibei Cui (3390248), Yong Huang (15745), Zengguo Wang (823502)   +10 more
core   +1 more source

Targeting the EpCAM‐AXL axis to overcome drug resistance in lung cancer

Molecular Oncology, EarlyView.
Lung cancer cells often evade therapy by hijacking signaling pathways. We reveal that cleaved EpCAM (sEpCAM) stabilizes the oncogenic protein AXL, driving NF‐κB and STAT3‐mediated chemoresistance. This EpCAM‐AXL axis identifies a high‐risk patient subset with poor prognosis.
Alexa Guerrero‐Alba, Mark Shacker, Timothy P. Fleming, Michael A. Smith, Ross M. Bremner, Narendra V. Sankpal   +5 more
wiley   +1 more source

Sensitive detection of novel Indian isolate of BTV 21 using ns1 gene based real-time PCR assay [PDF]

Veterinary World, 2013
Aim: The study was conducted to develop ns1 gene based sensitive real-time RT-PCR assay for diagnosis of India isolates of bluetongue virus (BTV). Materials and Methods: The BTV serotype 21 isolate (KMNO7) was isolated from Andhra Pradesh and propagated ...
Gaya Prasad, Pawan Kumar, Supriya A, P Minakshi, Ranjan Koushlesh, T.M. Vishwaradhya   +5 more
doaj   +1 more source

Characterization of therapeutic antibodies targeting lymphatic and vascular resistance factor for the treatment of hypervascularized and highly metastatic cancers

Molecular Oncology, EarlyView.
LVRF (lymphatic and vascular resistance factor) is a VEGFA splice variant upregulated in response to anti‐angiogenic therapy that fosters tumor adaptation through enhanced vascularization, lymphangiogenesis, and metastatic dissemination. Targeting LVRF with fully human neutralizing antibodies disrupts these processes and limits resistant tumor growth ...
Marion David, Arthur Karaulic, Audrey Bennetot, Clémence Fournier, Quentin Odet, Arthur Gouraud, Isabelle Bourget, Cédric Gaggioli, Sandy Giuliano, Frédéric Luciano, Olivier Léger, Zaki Sellam, Gilles Pagès   +12 more
wiley   +1 more source

Evaluation of real-time PCR targeting the lipL32 gene for diagnosis of Leptospira infection

BMC Microbiology, 2020
Background Different diagnostic methods have been used for the laboratory confirmation of leptospirosis. Molecular diagnostic techniques are not only faster and more sensitive than culture analysis, but can also detect a Leptospira infection before the ...
Daša Podgoršek, Eva Ružić-Sabljić, Mateja Logar, Andrea Pavlović, Tatjana Remec, Zvonko Baklan, Emil Pal, Tjaša Cerar   +7 more
doaj   +1 more source