Results 31 to 40 of about 3,251,371 (302)

RT-PCR cycle conditions.

, 2022
RT-PCR cycle conditions.
Tiago Souza Salles (11978685), Leandro Magalhães de Souza (11978694), Duleide Alves Pereira (11978709), Davis Fernandes Ferreira (11978718), Cinthya Domingues Amaral (11978703), José Antônio Suzano da Silva (11978700), Stefanella Boatto (11978712), Renata Campos Azevedo (11978721), Marcelo Damião Ferreira de Meneses (11978697), Jhonatan Ramos Felix (11978706), Fábio Burack da Costa (11978688), Maria Angélica Arpon Marandino Guimarães (11978715), Andrea Cony Cavalcanti (10373613), Vanessa Zaquieu Dias (11978691)   +13 more
core   +1 more source

Noninvasive prenatal testing of beta-thalassemia for common Pakistani mutations: a comparative study using cell-free fetal DNA from maternal plasma and chorionic villus sampling

Hematology, 2022
Background The discovery of circulating cell-free fetal DNA (cff-DNA) in maternal plasma has inspired the noninvasive prenatal testing (NIPT) approaches for various genetic fetal screening including rhesus D typing, sex determination, aneuploidies, and ...
Muhammad Afzal, Muhammad Abdul Naeem, Suhaib Ahmed, Nayyar Amin, Amena Rahim, Manazza Munawar, Mansoor Ishaq, Ali Rathore, K. Maria   +8 more
doaj   +1 more source

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR [PDF]

Journal of Visualized Experiments, 2018
The aim of this method is to facilitate the fast, sensitive and specific detection of Tilapia Lake Virus (TiLV) in tilapia tissues. This protocol can be used as part of surveillance programs, biosecurity measures and in TiLV basic research laboratories.
Nicholson P, Rawiwan P, Surachetpong W
openaire   +3 more sources

RT-hn-PCR-T results compared to FAT.

, 2020
RT-hn-PCR-T results compared to FAT.
Felana Suzah Rasolonjatovo (8544648), Lalaina Arivony Nomenjanahary (7354490), Hélène Guis (44769), Malavika Rajeev (8544651), Laurent Dacheux (181814), Soa Fy Andriamandimby (8544660), Girard Razafitrimo (8544654), Jean-Michel Heraud (228445), Jean Théophile Rafisandrantantsoa (8544657), Catherine Cêtre-Sossah (606622)   +9 more
core   +1 more source

Multi-annual performance evaluation of laboratories in post-mortem diagnosis of animal rabies: Which techniques lead to the most reliable results in practice?

PLoS Neglected Tropical Diseases, 2021
Rabies diagnosis proficiency tests on animal specimens using four techniques (FAT, RTCIT, conventional RT-PCR and real-time RT-PCR) were organised over 10 years (2009-2019). Seventy-three laboratories, of which 59% were from Europe, took part.
Emmanuelle Robardet, Alexandre Servat, Jonathan Rieder, Evelyne Picard-Meyer, Florence Cliquet   +4 more
doaj   +1 more source

RT-hn-PCR-FP results compared to FAT.

, 2020
RT-hn-PCR-FP results compared to FAT.
Felana Suzah Rasolonjatovo (8544648), Lalaina Arivony Nomenjanahary (7354490), Hélène Guis (44769), Malavika Rajeev (8544651), Laurent Dacheux (181814), Soa Fy Andriamandimby (8544660), Girard Razafitrimo (8544654), Jean-Michel Heraud (228445), Jean Théophile Rafisandrantantsoa (8544657), Catherine Cêtre-Sossah (606622)   +9 more
core   +1 more source

RT-PCR versus extraction-less RT-LAMP workflow.

, 2022
RT-PCR versus extraction-less RT-LAMP workflow.
Arun Manoharan (4622767), Francesco Elia Marino (12400634), Eric Proffitt (12400637), Eugene Joseph (757282)   +3 more
core   +1 more source

Real-Time Polymerase Chain Reaction: Applications in Diagnostic Microbiology

International Journal of Medical Students, 2013
The polymerase chain reaction (PCR) has revolutionized the detection of DNA and RNA. Real-Time PCR (RT-PCR) is becoming the gold standard test for accurate, sensitive and fast diagnosis for a large range of infectious agents.
Kordo B. A. Saeed, Nusreen S. Ahmad
doaj   +3 more sources

RT-PCR and PCR primers used for study.

, 2021
RT-PCR primers and PCR primers used for r10001, r04103, cy0428, and cy0575 are listed. Replicates for cy0428 and cy0575 were separated following vRNA isolation from plasma, while replicates for r10001 and r04103 were separated following RT-PCR ...
Shelby L. O’Connor (8872016), Dane D. Gellerup (3375140), Athena E. Golfinos (11785344), Vanessa Venturi (182536), Miles P. Davenport (8201889), Alexis J. Balgeman (8871995), Andrea M. Weiler (9553607), Ryan V. Moriarty (3158157), Jaffna Mathiaparanam (5899256), Brandon F. Keele (7450001), Hannah Schweigert (11785347), Thomas C. Friedrich (8321406)   +11 more
core   +1 more source

Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco [PDF]

BMC Research Notes, 2016
A rapid, sensitive, and specific molecular method for the diagnosis of infectious bronchitis virus (IBV) infection is important in curbing infectious bronchitis outbreaks in Morocco and other countries.In this study, an easy-to-perform SYBR green I real-time reverse transcriptase polymerase chain reaction (RT-PCR) targeting the nucleocapsid gene of IBV
Fellahi, Siham, Harrak, Mehdi El, Kuhn, Jens H., Sebbar, Ghizlane, Bouaiti, El Arbi, Khataby, Khadija, Fihri, Ouafae Fassi, Houadfi, Mohammed El, Ennaji, My Mustapha   +8 more
openaire   +2 more sources