Results 21 to 30 of about 3,251,371 (302)

Comparison of CBNAAT and conventional real time RT PCR for HIV 1 viral load testing

Indian Journal of Medical Microbiology, 2021
HIV viral load testing is now recommended for monitoring of anti-retroviral treatment failure in PLHIV. Xpert® HIV-1 Viral Load is a fully automated CB-NAAT. A reduced turnaround time leads to prompt clinical management. Hence the current study was undertaken to compare Xpert® HIV-1 Viral Load with the routinely used conventional real time RT PCR.The ...
Nayana A, Ingole, Gita, Nataraj
openaire   +2 more sources

Characteristics of primers used in both real-time RT-qPCR and conventional RT-PCR techniques.

, 2023
Characteristics of primers used in both real-time RT-qPCR and conventional RT-PCR techniques.
Oxana Groza (16502891), Helene Quenault (9754690), Emmanuelle Robardet (331148), Elena Velescu (8166210), Evelyne Picard-Meyer (331154), Nicolae Starciuc (16502894), Mihaela Anca Dascalu (16502885), Florentina Daraban Bocaneti (16502903), Edouard Hirchaud (204322), Florence Cliquet (331147), Yannick Blanchard (577101), Serghei Arseniev (16502888), Oana Irina Tanase (16502900), Alexandre Servat (572154), Vlad Vuta (16502897), Florica Barbuceanu (16492562)   +15 more
core   +1 more source

Comparison of the conventional multiplex RT–PCR, real time RT–PCR and Luminex xTAG^® RVP fast assay for the detection of respiratory viruses [PDF]

Journal of Medical Virology, 2015
Detection of respiratory viruses using polymerase chain reaction (PCR) is sensitive, specific and cost effective, having huge potential for patient management. In this study, the performance of an in‐house developed conventional multiplex RT–PCR (mRT–PCR), real time RT–PCR (rtRT–PCR) and Luminex xTAG® RVP fast assay (Luminex Diagnostics, Toronto ...
Manohar L, Choudhary, Siddharth P, Anand, Shamal A, Tikhe, Atul M, Walimbe, Varsha A, Potdar, Mandeep S, Chadha, Akhilesh C, Mishra   +6 more
openaire   +2 more sources

Performance of Chip Based Real Time RT- PCR (TrueNat) and Conventional Real Time RT-PCR for Detection of SARS-CoV-2

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2021
Introduction: Coronavirus Disease-2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is ravaging the globe due to its rapid spread. Since providing fast results is of critical importance in a time of shortage of medical personnel and beds in isolation wards and to ensure timely treatment for patients, developing
AMIYABALA SAHOO, ANURADHA SHULANIA, MALA CHHABRA, STUTI KANSRA, ARVIND ACHRA, KIRTI NIRMAL, SUPRIYA KATIYAR, NANDINI DUGGAL   +7 more
openaire   +2 more sources

RT-PCR and RT-ddPCR primers.

, 2023
Abbreviations: F, forward; R, reverse; RT-PCR, reverse transcription-PCR; RT-ddPCR, reverse transcription-droplet digital PCR. (XLSX)
Patrizia Luppi (15307197), Peter Drain (15307200), Erik A. Koppes (15307191), Donna B. Stolz (6901796), John R. Yates III (8072333), Robert D. Nicholls (15307203), Hyun Jung Park (8778488), James J. Moresco (569744), Dale W. Lewis (6862094), Jolene K. Diedrich (1235706), Susanne M. Gollin (5063618), Simon C. Watkins (39900), Ronald C. Wek (15169871), Marie A. Johnson (15307194)   +13 more
core   +1 more source

Application of Rapid and Reliable Detection of Cymbidium Mosaic Virus by Reverse Transcription Recombinase Polymerase Amplification Combined with Lateral Flow Immunoassay [PDF]

The Plant Pathology Journal, 2022
Cymbidium mosaic virus (CymMV) is one of economically important viruses that cause significant losses of orchids in the world. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay combined with a lateral flow ...
Do-Hyun Kim, Rae-Dong Jeong, Sena Choi, Ho-Jong Ju, Ju-Yeon Yoon   +4 more
doaj   +1 more source

Detection of Strawberry necrotic shock virus using conventional and TaqMan® quantitative RT-PCR [PDF]

Journal of Virological Methods, 2016
Graft-indexing of an advanced selection from the University of Florida strawberry breeding program produced virus-like symptoms on Fragaria vesca. However; RT-PCR testing of the material did not detect the presence of any of 16 strawberry virus species or members of virus groups for which strawberries are routinely indexed.
Thanuja Thekke Veetil, Thien Ho, Catalina Moyer, Vance M. Whitaker, Ioannis E. Tzanetakis   +4 more
openaire   +2 more sources

A comparative study on the use of real time polymerase chain reaction (RT-PCR) and standard isolation techniques for the detection of Salmonellae in broiler c

International Journal of Veterinary Science and Medicine, 2014
This study was carried out to compare between conventional cultural isolation methods and real time polymerase chain reaction (RT-PCR) technique for the detection of Salmonella in broiler chicks.
Waleed A. Ibrahim, Wafaa A. Abd El-Ghany, Soad A. Nasef, M.E. Hatem   +3 more
doaj   +1 more source

Quick assessment of influenza a virus infectivity with a long-range reverse-transcription quantitative polymerase chain reaction assay

BMC Infectious Diseases, 2020
Background The polymerase chain reaction (PCR) is commonly used to detect viral pathogens because of its high sensitivity and specificity. However, conventional PCR methods cannot determine virus infectivity.
Yuki Nakaya, Takashi Fukuda, Hiroki Ashiba, Masato Yasuura, Makoto Fujimaki   +4 more
doaj   +1 more source

Evaluation of Real-Time RT-PCR Compared with Conventional RT-PCR for Detecting Human Metapneumovirus RNA from Clinical Specimens

Japanese Journal of Infectious Diseases, 2008
Human metapneumovirus (hMPV) is an etiologic agent of respiratory tract infections. In this study, we compared the sensitivity and specificity of real-time reverse transcription (RT)-polymerase chain reaction (PCR), conventional RT-PCR, and nested PCR in detecting hMPV genes.
Kaida, Atsushi, Kubo, Hideyuki, Shiomi, Masashi, Kohdera, Urara, Iritani, Nobuhiro   +4 more
openaire   +2 more sources