Noroviruses Surrogate Detection Using Loop-Mediated Isothermal Amplification (LAMP), Conventional RT-PCR and Quantitative RT-PCR in Sg. Tekala and Sg. Gabai Stream Water [PDF]
MATEC Web of Conferences, 2017 A preliminary study was carried out in order to evaluate a reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detecting Noroviruses (NoV) RNA surrogates as external standard for NoV.The detection limit of the NoV RT-LAMP ...
Khairuddin Dzulaikha +3 more
doaj +5 more sources
Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A [PDF]
Ciência Rural, 2009 Avian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV. The aim of the present study was to compare the detection of six subgroup A AMPV isolates (AMPV/A) viral RNA by using different conventional and real time RT-PCR ...
Ferreira, Helena Lage +4 more
openaire +4 more sources
DETECTION SENSITIVITY AND QUANTITATION OF PLASMODIUM FALCIPARUM VAR GENE TRANSCRIPTS BY REAL-TIME RT-PCR IN COMPARISON WITH CONVENTIONAL RT-PCR [PDF]
The American Journal of Tropical Medicine and Hygiene, 2006 Antigenic variation in Plasmodium falciparum erythrocyte membrane protein 1, caused by a switch in transcription of the encoding var gene, is an important feature of malaria. In this study, we quantified the relative abundance of var gene transcripts present in P.
Gatton, Michelle L. +5 more
openaire +5 more sources
Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR [PDF]
Virology Journal, 2011 Background Bovine viral diarrhea virus (BVDV) is a worldwide pathogen in cattle and acts as a surrogate model for hepatitis C virus (HCV). One-step real-time fluorogenic quantitative reverse transcription polymerase chain reaction (RT-PCR) assay based on
Lou Sai +8 more
doaj +4 more sources
Gel express: a novel frugal method quantifies gene relative expression in conventional RT-PCR [PDF]
Beni-Suef University Journal of Basic and Applied Sciences, 2022 Background Real-time PCR system is a valuable scientific mainstream needed for quantifying specific gene expression. Nevertheless, compared with conventional PCR, the real-time PCR system is extremely expensive and not affordable for limited or mid ...
Mohamed Hazman
doaj +2 more sources
Ciência Rural, 2009 Avian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV.
Helena Lage Ferreira +4 more
doaj +1 more source
Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay. [PDF]
Biomed Res Int, 2018 Porcine deltacoronavirus (PDCoV) is a newly discovered coronavirus, which belongs to the family Coronaviridae. It causes watery diarrhea, vomiting, and dehydration in newborn piglets. A sensitive RT-PCR method is urgently required to detect PDCoV infection.
Ma L +6 more
europepmc +4 more sources
Human rhinovirus screening conventional RT-PCR ("Gama assay") v2
, 2018 This is a very handy, previously published [Ref 1 and 2], rhinovirus (RV) screening assay which I and many others have used on many sample extracts, mostly originating fromacutely ill paediatric patients, spanning well over a decade's worth of collection dates.
Ian M Mackay, Katherine E. Arden
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, 2022 Real-time quantitative polymerase chain reaction (real-time PCR) was designed for the detection of oil palm Coconut Cadang-cadang Viroid (CCCVd) 246 nt variant.
Roslan, Nur Diyana +5 more
core +1 more source