mOTUpan: a robust Bayesian approach to leverage metagenome-assembled genomes for core-genome estimation [PDF]
Recent advances in sequencing and bioinformatics have expanded the tree of life by providing genomes for uncultured environmentally relevant clades, either through metagenome assembled genomes (MAGs) or single-cell assembled genomes (SAGs).
M. Buck, M. Mehrshad, S. Bertilsson
semanticscholar +4 more sources
Using Core Genome Alignments To Assign Bacterial Species [PDF]
With the increasing availability of genome sequences, we sought to develop and apply a robust, portable, and high-resolution method for the assignment of genera and species designations that can recapitulate classically defined taxonomic designations ...
M. Chung +3 more
semanticscholar +5 more sources
Whole Genome and Core Genome Multilocus Sequence Typing and Single Nucleotide Polymorphism Analyses of Listeria monocytogenes Isolates Associated with an Outbreak Linked to Cheese, United States, 2013 [PDF]
Epidemiological findings of a listeriosis outbreak in 2013 implicated Hispanic-style cheese produced by company A, and pulsed-field gel electrophoresis (PFGE) and whole genome sequencing (WGS) were performed on clinical isolates and representative ...
Yi Chen +21 more
openalex +2 more sources
Antimicrobial Resistance Profile, Whole-Genome Sequencing and Core Genome Multilocus Sequence Typing of B. anthracis Isolates in Croatia from 2001 to 2022. [PDF]
Kompes G +8 more
europepmc +3 more sources
“Usage a CRISPR/Cas9-based for obtaining knockouts of economically significant cattle genes” [PDF]
An experimental work dealing with the gene modification using the Cas9 RNA-based editing system was performed. Point site-specific breakpoints in gDNA were introduced at the zygote stage by microinjection of spCas9 mRNA protein and guide RNAs into the ...
Bruter A. +5 more
doaj +1 more source
Gene editing CRISPR/Cas9 system for producing cows with hypoallergenic milk on the background of a beta-lactoglobulin gene knockout [PDF]
Beta-lactoglobulin is the main allergen of cow’s milk. Modern approaches to reducing the allergenicity of milk require significant costs for its fermentation. An alternative approach could be the creation of productive breeds with a knockout for the gene
Silaeva Yulia Yu. +4 more
doaj +1 more source
Generation of zebrafish (Danio rerio) models with targeted insertion of epitope tags and point mutations is highly desirable for functional genomics and disease modeling studies.
Blake Carrington, Raman Sood
doaj +1 more source
Development and implementation of a core genome multilocus sequence typing scheme for Yersinia enterocolitica: a tool for surveillance and outbreak detection. [PDF]
Pires J, Brandal LT, Naseer U.
europepmc +2 more sources
Background Archival formalin-fixed, paraffin-embedded (FFPE) tissue samples with clinical and histological data are a singularly valuable resource for developing new molecular biomarkers.
Jin Sung Jang +7 more
doaj +1 more source
Distinguishing individuals or small groups is essential for many experiments. The regenerative properties of zebrafish make traditional marking methods for rodent models (e.g., tattoos, tissue clipping) ineffective. Fluorescent Visible Implant Elastomer (
Stephen Frederickson +2 more
doaj +1 more source

