Results 11 to 20 of about 4,042,841 (387)

mOTUpan: a robust Bayesian approach to leverage metagenome-assembled genomes for core-genome estimation [PDF]

open access: yesNAR Genomics and Bioinformatics, 2021
Recent advances in sequencing and bioinformatics have expanded the tree of life by providing genomes for uncultured environmentally relevant clades, either through metagenome assembled genomes (MAGs) or single-cell assembled genomes (SAGs).
M. Buck, M. Mehrshad, S. Bertilsson
semanticscholar   +4 more sources

Using Core Genome Alignments To Assign Bacterial Species [PDF]

open access: yesmSystems, 2018
With the increasing availability of genome sequences, we sought to develop and apply a robust, portable, and high-resolution method for the assignment of genera and species designations that can recapitulate classically defined taxonomic designations ...
M. Chung   +3 more
semanticscholar   +5 more sources

Whole Genome and Core Genome Multilocus Sequence Typing and Single Nucleotide Polymorphism Analyses of Listeria monocytogenes Isolates Associated with an Outbreak Linked to Cheese, United States, 2013 [PDF]

open access: hybridApplied and Environmental Microbiology, 2017
Epidemiological findings of a listeriosis outbreak in 2013 implicated Hispanic-style cheese produced by company A, and pulsed-field gel electrophoresis (PFGE) and whole genome sequencing (WGS) were performed on clinical isolates and representative ...
Yi Chen   +21 more
openalex   +2 more sources

“Usage a CRISPR/Cas9-based for obtaining knockouts of economically significant cattle genes” [PDF]

open access: yesE3S Web of Conferences, 2023
An experimental work dealing with the gene modification using the Cas9 RNA-based editing system was performed. Point site-specific breakpoints in gDNA were introduced at the zygote stage by microinjection of spCas9 mRNA protein and guide RNAs into the ...
Bruter A.   +5 more
doaj   +1 more source

Gene editing CRISPR/Cas9 system for producing cows with hypoallergenic milk on the background of a beta-lactoglobulin gene knockout [PDF]

open access: yesE3S Web of Conferences, 2020
Beta-lactoglobulin is the main allergen of cow’s milk. Modern approaches to reducing the allergenicity of milk require significant costs for its fermentation. An alternative approach could be the creation of productive breeds with a knockout for the gene
Silaeva Yulia Yu.   +4 more
doaj   +1 more source

Fluorescent PCR–based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in Zebrafish

open access: yesBio-Protocol, 2023
Generation of zebrafish (Danio rerio) models with targeted insertion of epitope tags and point mutations is highly desirable for functional genomics and disease modeling studies.
Blake Carrington, Raman Sood
doaj   +1 more source

Application of the 3′ mRNA-Seq using unique molecular identifiers in highly degraded RNA derived from formalin-fixed, paraffin-embedded tissue

open access: yesBMC Genomics, 2021
Background Archival formalin-fixed, paraffin-embedded (FFPE) tissue samples with clinical and histological data are a singularly valuable resource for developing new molecular biomarkers.
Jin Sung Jang   +7 more
doaj   +1 more source

Zebrafish Injectable Plastic for Identification Tagging (ZIP IT) for larvae to adults using a fluorescent Visible Implant Elastomer

open access: yesMethodsX, 2023
Distinguishing individuals or small groups is essential for many experiments. The regenerative properties of zebrafish make traditional marking methods for rodent models (e.g., tattoos, tissue clipping) ineffective. Fluorescent Visible Implant Elastomer (
Stephen Frederickson   +2 more
doaj   +1 more source

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