Results 1 to 10 of about 558,772 (119)

“Usage a CRISPR/Cas9-based for obtaining knockouts of economically significant cattle genes” [PDF]

E3S Web of Conferences, 2023
An experimental work dealing with the gene modification using the Cas9 RNA-based editing system was performed. Point site-specific breakpoints in gDNA were introduced at the zygote stage by microinjection of spCas9 mRNA protein and guide RNAs into the ...
Bruter A., Okulova Yu., Lysikova E., Silaeva Yu., Makutina V., Krivonogova A.   +5 more
doaj   +1 more source

Gene editing CRISPR/Cas9 system for producing cows with hypoallergenic milk on the background of a beta-lactoglobulin gene knockout [PDF]

E3S Web of Conferences, 2020
Beta-lactoglobulin is the main allergen of cow’s milk. Modern approaches to reducing the allergenicity of milk require significant costs for its fermentation. An alternative approach could be the creation of productive breeds with a knockout for the gene
Silaeva Yulia Yu., Kubekina Marina V., Bruter Alexandra V., Isaeva Albina G., Koshchaev Andrey G.   +4 more
doaj   +1 more source

Identifying the core genome of the nucleus-forming bacteriophage family and characterization of Erwinia phage RAY [PDF]

Cell Reports, 2023
Joe Pogliano, Amy Prichard, Demyan Davydov   +2 more
exaly   +2 more sources

Fluorescent PCR–based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in Zebrafish

Bio-Protocol, 2023
Generation of zebrafish (Danio rerio) models with targeted insertion of epitope tags and point mutations is highly desirable for functional genomics and disease modeling studies.
Blake Carrington, Raman Sood
doaj   +1 more source

Application of the 3′ mRNA-Seq using unique molecular identifiers in highly degraded RNA derived from formalin-fixed, paraffin-embedded tissue

BMC Genomics, 2021
Background Archival formalin-fixed, paraffin-embedded (FFPE) tissue samples with clinical and histological data are a singularly valuable resource for developing new molecular biomarkers.
Jin Sung Jang, Eileen Holicky, Julie Lau, Samantha McDonough, Mark Mutawe, Matthew J. Koster, Kenneth J. Warrington, Julie M. Cuninngham   +7 more
doaj   +1 more source

Zebrafish Injectable Plastic for Identification Tagging (ZIP IT) for larvae to adults using a fluorescent Visible Implant Elastomer

MethodsX, 2023
Distinguishing individuals or small groups is essential for many experiments. The regenerative properties of zebrafish make traditional marking methods for rodent models (e.g., tattoos, tissue clipping) ineffective. Fluorescent Visible Implant Elastomer (
Stephen Frederickson, Blake Carrington, Tannia Clark   +2 more
doaj   +1 more source

Aggregation and disaggregation features of the human proteome

Molecular Systems Biology, 2020
Protein aggregates have negative implications in disease. While reductionist experiments have increased our understanding of aggregation processes, the systemic view in biological context is still limited.
Tomi A Määttä, Mandy Rettel, Sindhuja Sridharan, Dominic Helm, Nils Kurzawa, Frank Stein, Mikhail M Savitski   +6 more
doaj   +1 more source

Thermal proteome profiling for interrogating protein interactions

Molecular Systems Biology, 2020
Thermal proteome profiling (TPP) is based on the principle that, when subjected to heat, proteins denature and become insoluble. Proteins can change their thermal stability upon interactions with small molecules (such as drugs or metabolites), nucleic ...
André Mateus, Nils Kurzawa, Isabelle Becher, Sindhuja Sridharan, Dominic Helm, Frank Stein, Athanasios Typas, Mikhail M Savitski   +7 more
doaj   +1 more source

BE4max and AncBE4max Are Efficient in Germline Conversion of C:G to T:A Base Pairs in Zebrafish

Cells, 2020
The ease of use and robustness of genome editing by CRISPR/Cas9 has led to successful use of gene knockout zebrafish for disease modeling. However, it still remains a challenge to precisely edit the zebrafish genome to create single-nucleotide ...
Blake Carrington, Rachel N. Weinstein, Raman Sood   +2 more
doaj   +1 more source

5’isomiR-183-5p|+2 elicits tumor suppressor activity in a negative feedback loop with E2F1

Journal of Experimental & Clinical Cancer Research, 2022
Background MicroRNAs (miRNAs) and isomiRs play important roles in tumorigenesis as essential regulators of gene expression. 5’isomiRs exhibit a shifted seed sequence compared to the canonical miRNA, resulting in different target spectra and thereby ...
Xiaoya Li, Birgitta Elisabeth Michels, Oyku Ece Tosun, Janine Jung, Jolane Kappes, Susanne Ibing, Nishanth Belugali Nataraj, Shashwat Sahay, Martin Schneider, Angelika Wörner, Corinna Becki, Naveed Ishaque, Lars Feuerbach, Bernd Heßling, Dominic Helm, Rainer Will, Yosef Yarden, Karin Müller-Decker, Stefan Wiemann, Cindy Körner   +19 more
doaj   +1 more source