Thermal proteome profiling in bacteria: probing protein state in vivo [PDF]
Molecular Systems Biology, 2018 Increasing antibiotic resistance urges for new technologies for studying microbes and antimicrobial mechanism of action. We adapted thermal proteome profiling (TPP) to probe the thermostability of Escherichia coli proteins in vivo. E.
André Mateus +7 more
doaj +7 more sources
A Bayesian semi-parametric model for thermal proteome profiling [PDF]
Communications Biology, 2021 Fang et al. develop a Bayesian data analysis approach that is better suited to the analysis of Thermal Proteome Profiling (TPP) data than existing data analysis approaches that have limitations with respect to deviations from the expected sigmoid data ...
Siqi Fang +4 more
doaj +10 more sources
Thermal proteome profiling for interrogating protein interactions [PDF]
Molecular Systems Biology, 2020 Thermal proteome profiling (TPP) is based on the principle that, when subjected to heat, proteins denature and become insoluble. Proteins can change their thermal stability upon interactions with small molecules (such as drugs or metabolites), nucleic ...
André Mateus +7 more
doaj +7 more sources
Utilizing thermal proteome profiling to identify the molecular targets of anti-leishmanial compounds [PDF]
STAR Protocols, 2021 Summary: Here, we detail our optimized protocol for the identification of drug targets in Leishmania donovani using thermal proteome profiling. This approach is based on the principle that binding of a drug to its protein target can significantly alter ...
Victoriano Corpas-Lopez, Susan Wyllie
doaj +8 more sources
Mutant thermal proteome profiling for characterization of missense protein variants and their associated phenotypes within the proteome [PDF]
Journal of Biological Chemistry, 2020 Temperature-sensitive (TS) missense mutants have been foundational for characterization of essential gene function. However, an unbiased approach for analysis of biochemical and biophysical changes in TS missense mutants within the context of their functional proteomes is lacking.
Amber L Mosley +2 more
exaly +7 more sources
Comparison of Quantitative Mass Spectrometric Methods for Drug Target Identification by Thermal Proteome Profiling [PDF]
Journal of Proteome Research, 2023 Abstract Thermal proteome profiling (TPP) provides a powerful approach to studying proteome-wide interactions of small therapeutic molecules and their target and off-target proteins, complementing phenotypic-based drug screens. Detecting differences in thermal stability due to target engagement requires high quantitative accuracy and ...
Jessica Watt +2 more
exaly +10 more sources
Thermal Proteome Profiling Reveals the O-GlcNAc-Dependent Meltome. [PDF]
J Am Chem Soc, 2022 Posttranslational modifications alter the biophysical properties of proteins and thereby influence cellular physiology. One emerging manner by which such modifications regulate protein functions is through their ability to perturb protein stability.
King DT +6 more
europepmc +13 more sources
Thermal Proteome Profiling to Identify Protein-ligand Interactions in the Apicomplexan Parasite Toxoplasma gondii [PDF]
Bio-Protocol, 2021 Toxoplasma gondii is a single-celled eukaryotic parasite that chronically infects a quarter of the global population. In recent years, phenotypic screens have identified compounds that block parasite replication.
Alice Herneisen, Sebastian Lourido
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Inflect: Optimizing Computational Workflows for Thermal Proteome Profiling Data Analysis. [PDF]
J Proteome Res, 2021 ABSTRACT The use of CETSA and Thermal Proteome Profiling (TPP) analytical methods are invaluable for the study of protein-ligand interactions and protein stability in a cellular context. These tools have increasingly been leveraged in work ranging from understanding signaling paradigms to drug discovery. Consequently,
McCracken NA +3 more
europepmc +7 more sources
The Introduction of Detergents in Thermal Proteome Profiling Requires Lowering the Applied Temperatures for Efficient Target Protein Identification [PDF]
Molecules, 2023 Although the use of detergents in thermal proteome profiling (TPP) has become a common practice to identify membrane protein targets in complex biological samples, surprisingly, there is no proteome-wide investigation into the impacts of detergent ...
Yuying Ye +7 more
doaj +4 more sources