Results 21 to 30 of about 6,447 (219)

Label-Free Quantitative Thermal Proteome Profiling Reveals Target Transcription Factors with Activities Modulated by MC3R Signaling. [PDF]

Anal Chem, 2023
Thermal proteome profiling with label-free quantitation using ion-mobility-enhanced LC–MS offers versatile data sets, providing information on protein differential expression, thermal stability, and the activities of transcription factors. We developed a
Sandbaumhüter FA, Nezhyva M, Andrén PE, Jansson ET.   +3 more
europepmc   +18 more sources

Thermal proteome profiling efficiently identifies ribosome destabilizing oxazolidinones [PDF]

Tetrahedron, 2021
Abstract Identifying the targets of bioactive small molecules is a challenging endeavor for which no general solution currently exists. Classical affinity purification experiments suffer from the need to functionalise a bioactive compound and link it to a solid support, which may interfere with target binding.
Christina Nöcker, Nadine Kaiser, Daniel Foley, Sonja Sievers, Petra Janning, Herbert Waldmann, Luca Laraia   +6 more
openaire   +3 more sources

Thermal proteome profiling reveals Haemonchus orphan protein HCO_011565 as a target of the nematocidal small molecule UMW-868 [PDF]

Frontiers in Pharmacology, 2022
Parasitic roundworms (nematodes) cause destructive diseases, and immense suffering in humans and other animals around the world. The control of these parasites relies heavily on anthelmintic therapy, but treatment failures and resistance to these drugs ...
Aya C. Taki, Tao Wang, Nghi N. Nguyen, Ching-Seng Ang, Michael G. Leeming, Shuai Nie, Joseph J. Byrne, Neil D. Young, Yuanting Zheng, Guangxu Ma, Guangxu Ma, Pasi K. Korhonen, Anson V. Koehler, Nicholas A. Williamson, Andreas Hofmann, Bill C. H. Chang, Cécile Häberli, Cécile Häberli, Jennifer Keiser, Jennifer Keiser, Abdul Jabbar, Brad E. Sleebs, Brad E. Sleebs, Brad E. Sleebs, Robin B. Gasser   +24 more
doaj   +2 more sources

Application of Bioactive Thermal Proteome Profiling to Decipher the Mechanism of Action of the Lipid Lowering 13²-Hydroxy-pheophytin Isolated from a Marine Cyanobacteria [PDF]

Marine Drugs, 2019
The acceleration of the process of understanding the pharmacological application of new marine bioactive compounds requires identifying the compound protein targets leading the molecular mechanisms in a living cell.
Ana Carrasco del Amor, Sara Freitas, Ralph Urbatzka, Olatz Fresnedo, Susana Cristobal   +4 more
doaj   +2 more sources

Thermal Proteome Profiling Reveals Meltome Upon NLRP3 Inflammasome Activation. [PDF]

Mol Cell Proteomics
NOD-like receptor (NLR) family pyrin domain containing 3 (NLRP3) involves in inflammasome complex assembly and innate immunity. Activation of the NLRP3 inflammasome induces conformational alterations in protein complexes, influencing their interactions with other molecules, which in turn affects protein thermal stability.
Yang C, Wang L, Liu Y, Zhang Y, Jin C, Cheng J, Shang L, Fang L, Wu S, Chen C, Wang J.   +10 more
europepmc   +3 more sources

Putative identification of CASTOR1 as one of the targets of ganoderic acid a via thermal proteome profiling and molecular docking [PDF]

Frontiers in Pharmacology
In traditional Chinese medicine theory, aging is hypothesized to arise from severe deficiency of kidney essence, which is posited to induce “emptiness of the sea of marrow”.
Aifang Chen, Weishuai Xu, Yaxin Wang, Hualun Liang, Diling Chen   +4 more
doaj   +2 more sources

Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1 [PDF]

Nature Communications
Deep understanding of sugar metabolite-protein interactions should provide implications on sugar metabolic reprogramming in human physiopathology. Although tremendous efforts have been made for determining individual event, global profiling of such ...
Yanling Zhang, Yafei Cao, Xia Wu, Zhenghui Chen, Bowen Chen, Anhui Wang, Yanshen Guo, Wei Chen, Ruolan Xue, Zihua Liu, Yuanpei Li, Tian Li, Ruiqin Cheng, Ning Zhou, Jing Li, Yuan Liu, Xiaohui Zhao, Huixin Luo, Ming Xu, Houhua Li, Yiqun Geng   +20 more
doaj   +2 more sources

Thermal proteome profiling and proteome analysis using high‐definition mass spectrometry demonstrate modulation of cholesterol biosynthesis by next‐generation galeterone analog VNPP433‐3β in castration‐resistant prostate cancer [PDF]

Molecular Oncology
Cholesterol (CHOL) homeostasis is significantly modulated in prostate cancer (PCa) suggesting an active role in PCa development and progression. Several studies indicate a strong correlation between elevated CHOL levels and increased PCa risk and ...
Retheesh S. Thankan, Elizabeth Thomas, Mehari M. Weldemariam, Puranik Purushottamachar, Weiliang Huang, Maureen A. Kane, Yuji Zhang, Nicholas Ambulos, Bi‐Dar Wang, David Weber, Vincent C. O. Njar   +10 more
doaj   +2 more sources

Salmonella infection impacts host proteome thermal stability [PDF]

European Journal of Cell Biology
Intracellular bacterial pathogens hijack the protein machinery of infected host cells to evade their defenses and cultivate a favorable intracellular niche. The intracellular pathogen Salmonella enterica subsp.
Marlène S. Birk, Philipp Walch, Tarik Baykara, Stephanie Sefried, Jan Amelang, Elena Buerova, Ingrid Breuer, Jörg Vervoorts, Athanasios Typas, Mikhail M. Savitski, André Mateus, Joel Selkrig   +11 more
doaj   +2 more sources

Obtaining Functional Proteomics Insights From Thermal Proteome Profiling Through Optimized Melt Shift Calculation and Statistical Analysis With InflectSSP [PDF]

Molecular and Cellular Proteomics, 2023
Amber L Mosley, Avery Runnebohm, Aruna B Wijeratne   +2 more
exaly   +2 more sources