Results 41 to 50 of about 6,447 (219)
Nature Communications, 2023 Protein N-terminal (Nt) acetylation is one of the most abundant modifications in eukaryotes, covering ~50-80 % of the proteome, depending on species. Cells with defective Nt-acetylation display a wide array of phenotypes such as impaired growth, mating ...
Ulises H. Guzman +7 more
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Thermal proteome profiling reveals distinct target selectivity for differentially oxidized oxysterols [PDF]
ACS Chemical Biology, 2021 Abstract Oxysterols are produced physiologically by many species, however their distinct roles in regulating human (patho)physiology have not been studied systematically. The role of differing oxidation states and sites in mediating their biological functions is also unclear.
Cecilia Rossetti, Luca Laraia
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The pharmacology and therapeutic role of cannabidiol in diabetes
Exploration, 2023 In recent years, cannabidiol (CBD), a non‐psychotropic cannabinoid, has garnered substantial interest in drug development due to its broad pharmacological activity and multi‐target effects. Diabetes is a chronic metabolic disease that can damage multiple
Jin Zhang +9 more
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Nature Communications, 2020 2D-thermal proteome profiling (2D-TPP) is a powerful assay for probing interactions of proteins with small molecules in their native context. Here the authors provide a statistical method for false discovery rate controlled analysis for 2D-TPP ...
Nils Kurzawa +8 more
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SARS‐CoV‐2 infection remodels the host protein thermal stability landscape
Molecular Systems Biology, 2021 The severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is a global threat to human health and has compromised economic stability. In addition to the development of an effective vaccine, it is imperative to understand how SARS‐CoV‐2 hijacks host
Joel Selkrig +19 more
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Elucidating the Mechanisms of Action of Antimicrobial Agents
mBio, 2022 Despite the ever-growing antibiotic resistance crisis, the rate at which new antimicrobials are being discovered and approved for human use has rapidly declined over the past 75 years. A barrier for advancing newly identified antibiotics beyond discovery
M. Ashley Hudson, Steve W. Lockless
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Bio-Protocol, 2022 Chemical proteomics focuses on the drug–target–phenotype relationship for target deconvolution and elucidation of the mechanism of action—key and bottleneck in drug development and repurposing.
Xuepei Zhang +4 more
doaj +1 more source
Thermal proteome profiling reveals distinct target selectivity for differentially oxidized oxysterols [PDF]
, 2022 The dataset contains excel macro used for the data analysis from Thermal Proteomic Profiling exeriments aimed to identify the protein targets of three A- and B-ring oxidized sterols (cholestane-3β,5α,6β-triol (CT), 7keto-cholesterol (7-KC), 4ß ...
Rossetti, C (via Mendeley Data)
core +1 more source
Frontiers in Molecular Biosciences, 2022 Knowing that the drug candidate binds to its intended target is a vital part of drug discovery. Thus, several labeled and label-free methods have been developed to study target engagement.
Tuomas Aleksi Tolvanen +1 more
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Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling [PDF]
Nature Methods, 2015 Thermal stabilization of proteins after ligand binding provides an efficient means to assess the binding of small molecules to proteins. We show here that in combination with quantitative mass spectrometry, the approach allows for the systematic survey of protein engagement by cellular metabolites and drugs.
Kilian V M Huber +6 more
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