Nonionic Surfactants can Modify the Thermal Stability of Globular and Membrane Proteins Interfering with the Thermal Proteome Profiling Principles to Identify Protein Targets. [PDF]
Anal Chem, 2023 The membrane proteins are essential targets for understanding cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system-level understanding of cellular response to stimuli or perturbations.
Berlin E +4 more
europepmc +3 more sources
Proteome-Wide Deconvolution of Drug Targets and Binding Sites by Lysine Reactivity Profiling [PDF]
, 2022 Recently, numerous efforts have been devoted to identifying drug targets and binding sites in complex proteomes, which is of great importance in modern drug discovery.
Mingliang Ye (19659) +6 more
core +5 more sources
Molecular crowding effects on protein stability in a bacterial proteome [PDF]
Scientific ReportsMolecular crowding refers to the restriction of space available for molecular mobility in solution due to the presence of other macromolecules. Crowding can influence biological phenomena such as protein stability and folding, diffusion, enzyme kinetics,
Kate McKeever +3 more
doaj +2 more sources
Precipitate-Supported Thermal Proteome Profiling Coupled with Deep Learning for Comprehensive Screening of Drug Target Proteins [PDF]
, 2022 Although thermal proteome profiling (TPP) acts as a popular modification-free approach for drug target deconvolution, some key problems are still limiting screening sensitivity.
Yongjun Dang (269142) +8 more
core +11 more sources
Mitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteins
Life Science Alliance, 2023 The combination of thermal proteome profiling and pulsed SILAC labelling shows that mitochondrial dysfunction imprints a characteristic pattern on the abundance and thermal stability of the proteome.
Carina Groh +7 more
doaj +1 more source
Metal Extraction Triggered Agitation Logged by Thermal Proteome Profiling [PDF]
, 2023 <p>Metal Extraction Triggered Agitation Logged by Thermal Proteome Profiling (METAL-TPP) is designed for global discovery of MBPs in proteomes, which operates by extracting metals from MBPs with chelators and logging the resulting structural ...
Yuan, Liu
core +1 more source
Quantitative Proteomics Explore the Potential Targets and Action Mechanisms of Hydroxychloroquine
Molecules, 2022 Hydroxychloroquine (HCQ) is an autophagy inhibitor that has been used for the treatment of many diseases, such as malaria, rheumatoid arthritis, systemic lupus erythematosus, and cancer.
Jingxiang Zhao +9 more
doaj +1 more source
eLife, 2022 Apicomplexan parasites cause persistent mortality and morbidity worldwide through diseases including malaria, toxoplasmosis, and cryptosporidiosis. Ca2+ signaling pathways have been repurposed in these eukaryotic pathogens to regulate parasite-specific ...
Alice L Herneisen +4 more
doaj +1 more source
Assessing target engagement using proteome-wide solvent shift assays
eLife, 2021 Recent advances in mass spectrometry (MS) have enabled quantitative proteomics to become a powerful tool in the field of drug discovery, especially when applied toward proteome-wide target engagement studies.
Jonathan G Van Vranken +4 more
doaj +1 more source
Assessing Target Specificity of the Small Molecule Inhibitor MARIMASTAT to Snake Venom Toxins: A Novel Application of Thermal Proteome Profiling. [PDF]
Mol Cell Proteomics21 páginas, 8 figuras.New treatments that circumvent the pitfalls of traditional antivenom therapies are critical to address the problem of snakebite globally.
Smith CF +15 more
europepmc +3 more sources