Results 91 to 100 of about 1,203,995 (286)

Correlative light and volume electron microscopy to study brain development

Recent advances in volume electron microscopy (EM) have been driving our thorough understanding of the brain architecture. Volume EM becomes increasingly powerful when cells and their subcellular structures that are imaged in light microscopy are ...
Hayashi, Shuichi, Ohno, Nobuhiko, Molnar, Zoltan, Knott, Graham   +3 more
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PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy [PDF]

, 2019
Cryo-Electron Tomography (cryo-ET) is emerging as a revolutionary method for resolving the structure of macromolecular complexes in situ. However, sample preparation for in situ Cryo-ET is labour-intensive and can require both cryo-lamella preparation ...
Gorelick, Sergey, Johnson, Travis, de Marco, Alex, Gervinskas, Gediminas, Whisstock, James, Handley, Ava, Whisstock, James C, Pocock, Roger, Caggiano, Monica Pia, Johnson, Travis K, Buckley, Genevieve, Pocock, Roger; id_orcid   +11 more
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The VHL tumor suppressor at the crossroad of protein folding, aggregation, and cancer

Molecular Oncology, EarlyView.
Mutations, environmental stress, and chaperone dysfunction can destabilize pVHL, promoting its conversion from the native folded state into amyloid‐like assemblies. This transition may contribute to protein storage, cell dormancy, survival, and drug resistance.
Lara Abad, Silvio Carlo Ermanno Tosatto, Emanuela Leonardi   +2 more
wiley   +1 more source

Correlative Multi-Modal Microscopy: A Novel Pipeline for Optimizing Fluorescence Microscopy Resolutions in Biological Applications

, 2023
The modern fluorescence microscope is the convergence point of technologies with different performances in terms of statistical sampling, number of simultaneously analyzed signals, and spatial resolution. However, the best results are usually obtained by
Simone Pelicci, Laura Furia, Mario Faretta, Pier Giuseppe Pelicci, Faretta, Mario, Furia, Laura, Pelicci, Simone, Pelicci, Pier Giuseppe   +7 more
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Extracellular vesicle lipidomics liquid biopsy reveals systemic and tumor microenvironment metabolic reprogramming in ovarian cancer

Molecular Oncology, EarlyView.
Extracellular vesicle (EV) lipidomic profiling of plasma and ascites from patients with high‐grade serous ovarian cancer and of ovarian cancer cell lines reveals enrichment of triglycerides (TGs), diacylglycerols (DGs), phosphatidylcholine (PCs), sphingomyelins (SMs), reflecting tumor metabolic reprogramming.
Shikha Rani, Dominic Guanzon, Andrew Lai, Katherin Scholz‐Romero, Sarah Reed, Edita Ritmejeryte, Rohan Lourie, Naven Chetty, Kaltin Ferguson, Carlos Salomon   +9 more
wiley   +1 more source

NAPRT loss promotes lung tumor initiation and growth through AKT signaling independently of NAD+ biosynthesis

Molecular Oncology, EarlyView.
Loss of NAPRT promotes lung tumor initiation and growth through a noncanonical mechanism, independent of its role in NAD+ biosynthesis. Mechanistically, NAPRT depletion activates the mTORC2‐driven AKT/β‐catenin signaling axis to enhance clonogenic and invasive phenotypes. Furthermore, lung‐specific Naprt deletion significantly increases tumor burden in
Myung Joon Oh, Ji Yun Jang, Yoon Jeon, Gi‐Cheon Kim, Hwanho Lee, Jooyoung Lee, Ju‐Hwa Kim, Hakhyun Kim, Minjee Kim, Ho‐Keun Kwon, Ho Lee, Hyun Seok Kim   +11 more
wiley   +1 more source

Correlative light-electron fractography for fatigue striations characterization in metallic alloys

, 2013
The correlative light-electron fractography technique combines correlative microscopy concepts to the extended depth-from-focus reconstruction method, associating the reliable topographic information of 3-D maps from light microscopy ordered Z-stacks to ...
Campos, Kamila Amato de, Kamila Amato de Campos, Hein, Luis Rogerio de Oliveira, Oliveira, José Alberto de [UNESP], Luis Rogerio de Oliveira Hein, Oliveira, José Alberto de, Hein, Luis Rogerio de Oliveira [UNESP], José Alberto de Oliveira, Campos, Kamila Amato de [UNESP]   +8 more
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Correlative fractography: Combining scanning electron microscopy and light microscopes for qualitative and quantitative analysis of fracture surfaces [PDF]

, 2013
Correlative fractography is a new expression proposed here to describe a new method for the association between scanning electron microscopy (SEM) and light microscopy (LM) for the qualitative and quantitative analysis of fracture surfaces.
De Oliveira Hein, Luis Rogerio. [UNESP], De Oliveira, José Alberto [UNESP], De Campos, Kamila Amato, De Oliveira Hein, Luis Rogerio., De Campos, Kamila Amato [UNESP], De Oliveira, José Alberto   +5 more
core   +1 more source

Regulation of the lncRNA NEAT1 by p53‐ΔNp63 crosstalk modulates the DNA damage response and therapeutic efficacy in HNSCC

Molecular Oncology, EarlyView.
In head and neck squamous cell carcinoma (HNSCC) p53 and p63 exert opposite roles on the transcription regulation of the lncRNA NEAT1. Under basal conditions, p53 levels are low and p63 represses NEAT1 expression. Upon genotoxic stress, p53 is rapidly induced, displacing p63 from the NEAT1 promoter leading to NEAT1 transcriptional activation and ...
Sara De Domenico, Veronica La Banca, Silvia D'Amico, Stefano Scalera, Sara Nicolai, Angelo Peschiaroli   +5 more
wiley   +1 more source

A precise and rapid mapping protocol for correlative light and electron microscopy of small invertebrate organisms

, 2010
BACKGROUND INFORMATION: CLEM (correlative live cell and electron microscopy) seeks to bridge the data acquired with different imaging strategies, typically between light microscopy and electron microscopy.
Schwab, Yannick, Kolotueva, Irina, Labouesse, Michel, Irina Kolotuev, Michel Labouesse, Yannick Schwab   +5 more
core   +1 more source