Evidence for corrin biosynthesis in the last universal common ancestor [PDF]
The FEBS Journal, Volume 292, Issue 4, Page 827-850, February 2025.Cobalamin biosynthesis can be traced back to the last universal common ancestor. After a stage of chemical evolution of the acetyl‐CoA pathway, the enzymatic synthesis of cobalamin evolved, allowing life to be independent of the insoluble solid‐state catalysts that tethered primordial CO2 assimilation to the Earth's crust, suggesting a role for corrin ...
Luca D. Modjewski +5 more
wiley +2 more sources
Isolation and characterization of a novel methanogen Methanosarcina hadiensis sp. nov. from subsurface Boom Clay pore water [PDF]
Environmental Microbiology, Volume 26, Issue 12, December 2024.We isolated a novel methanogenic archaeon, Methanosarcina hadiensis sp. nov. (TD41E1‐1), from Boom Clay pore water at a depth of 225 m. It exhibits unique characteristics compared to previously characterized Methanosarcina species, such as the inability to perform acetoclastic methanogenesis and the presence of extensive surface appendages.
Francisco Javier Giménez +4 more
wiley +2 more sources
Exploitation of a Type 1 Toxin–Antitoxin System as an Inducible Counter-Selective Marker for Genome Editing in the Acetogen Eubacterium limosum [PDF]
Microorganisms, 2023 Targeted mutations in the anaerobic methylotroph Eubacterium limosum have previously been obtained using CRISPR-based mutagenesis methods.
James Millard +4 more
doaj +2 more sources
Skin-associated Corynebacterium amycolatum shares cobamides [PDF]
mSphereThe underlying interactions that occur to maintain skin microbiome composition, function, and overall skin health are largely unknown. Often, these types of interactions are mediated by microbial metabolites.
M. H. Swaney +4 more
doaj +2 more sources
Lettuce fortification through vitamin B12‐producing bacteria – proof of concept study [PDF]
Journal of the Science of Food and Agriculture, Volume 105, Issue 6, Page 3343-3354, April 2025.Abstract BACKGROUND Vitamin B12 (cobalamin) can be produced de novo only by certain bacteria and archaea. It plays a crucial role in the health of animals and humans, which obtain it only through diet, mainly from animal products. This study aimed to identify endophytic bacterial strains capable of synthesizing vitamin B12 and enriching edible plants ...
Sara Pipponzi +6 more
wiley +2 more sources
Site-specific incorporation of 19F-nuclei at protein C-terminus to probe allosteric conformational transitions of metalloproteins [PDF]
Communications BiologyAllosteric conformational change is an important paradigm in the regulation of protein function, which is typically triggered by the binding of small cofactors, metal ions or protein partners.
Xichun Liu +6 more
doaj +2 more sources
Soil microbial community response to corrinoids is shaped by a natural reservoir of vitamin B12 [PDF]
ISME JZachary F. Hallberg +9 more
openalex +2 more sources
Novel Pathway for Corrinoid Compounds Production in Lactobacillus
Frontiers in Microbiology, 2018 Vitamin B12 or cobalamin is an essential metabolite for humans, which makes it an interesting compound for many research groups that focus in different producer-strains synthesis pathways.
Andrea Carolina Torres +4 more
doaj +1 more source
Scientific Reports, 2023 Despite the success of AlphaFold2 (AF2), it is unclear how AF2 models accommodate for ligand binding. Here, we start with a protein sequence from Acidimicrobiaceae TMED77 (T7RdhA) with potential for catalyzing the degradation of per- and polyfluoroalkyl ...
Hao-Bo Guo +12 more
doaj +1 more source
Human gut Bacteroides capture vitamin B12 via cell surface-exposed lipoproteins
eLife, 2018 Human gut Bacteroides use surface-exposed lipoproteins to bind and metabolize complex polysaccharides. Although vitamins and other nutrients are also essential for commensal fitness, much less is known about how commensal bacteria compete with each other
Aaron G Wexler +5 more
doaj +1 more source