Cobalamin Riboswitches Are Broadly Sensitive to Corrinoid Cofactors to Enable an Efficient Gene Regulatory Strategy [PDF]
mBio, 2022 In bacteria, many essential metabolic processes are controlled by riboswitches, gene regulatory RNAs that directly bind and detect metabolites. Highly specific effector binding enables riboswitches to respond to a single biologically relevant metabolite.
Kristopher J. Kennedy +6 more
doaj +4 more sources
Genetic dissection of regulation by a repressing and novel activating corrinoid riboswitch enables engineering of synthetic riboswitches [PDF]
mBio, 2023 The ability to sense and respond to intracellular metabolite levels enables cells to adapt to environmental conditions. Many prokaryotes use riboswitches—structured RNA elements usually located in the 5′ untranslated region of mRNAs—to sense ...
Rebecca R. Procknow +4 more
doaj +3 more sources
Protein binding assays for an accurate differentiation of vitamin B12 from its inactive analogue. A study on edible cricket powder [PDF]
Food Chemistry: X, 2023 Inactive analogues of vitamin B12 (cobalamin, Cbl) can mimic the active Cbl in food if using the traditional microbiological measurements. Thus, overestimated Cbl was recently revealed in edible insects employing immunoaffinity adsorption, HPLC ...
Sergey N. Fedosov +4 more
doaj +2 more sources
Soil microbial community response to corrinoids is shaped by a natural reservoir of vitamin B12. [PDF]
ISME JHallberg ZF +9 more
europepmc +4 more sources
Genetic Analysis of Citrobacter sp.86 Reveals Involvement of Corrinoids in Chlordecone and Lindane Biotransformations [PDF]
Frontiers in Microbiology, 2020 Chlordecone (Kepone®) and γ-hexachlorocyclohexane (γ-HCH or lindane) have been used for decades in the French West Indies (FWI) resulting in long-term soil and water pollution. In a previous work, we have identified a new Citrobacter species (sp.86) that
Agnès Barbance +11 more
doaj +2 more sources
Cobamide metabolism, regulation, and adaptation in Mycobacterium tuberculosis [PDF]
Journal of BacteriologyCobamides play a paradoxical but critical role in the biology of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis. Although Mtb retains nearly all cobalamin (Cbl) biosynthetic genes and encodes multiple cobamide-requiring enzymes ...
Terry Kipkorir +4 more
doaj +2 more sources
Identification of a Novel Cobamide Remodeling Enzyme in the Beneficial Human Gut Bacterium
mBio, 2020 The beneficial human gut bacterium Akkermansia muciniphila provides metabolites to other members of the gut microbiota by breaking down host mucin, but most of its other metabolic functions have not been investigated. A.
Kenny C. Mok +6 more
doaj +2 more sources
Resonance Raman Optical Activity Spectroscopy in Probing Structural Changes Invisible to Circular Dichroism Spectroscopy: A Study on Truncated Vitamin B12 Derivatives [PDF]
Molecules, 2020 This work demonstrates resonance Raman optical activity (RROA) spectra of three truncated vitamin B12 derivatives modified within the nucleotide loop. Since truncated cobalamins possess sufficiently high rotational strength in the range of ROA excitation
Ewa Machalska +5 more
doaj +2 more sources
Genome-Based Metabolic Reconstruction Unravels the Key Role of B12 in Methionine Auxotrophy of an Ortho-Phenylphenol-Degrading Sphingomonas haloaromaticamans [PDF]
Frontiers in Microbiology, 2020 Auxotrophy to amino acids and vitamins is a common feature in the bacterial world shaping microbial communities through cross-feeding relations. The amino acid auxotrophy of pollutant-degrading bacteria could hamper their bioremediation potential ...
Chiara Perruchon +3 more
doaj +2 more sources
Corrinoids in anaerobic bacteria [PDF]
FEMS Microbiology Letters, 1990 Erhard Stupperich
exaly +2 more sources