A hybrid multi-object segmentation framework with model-based B-splines for microbial single cell analysis [PDF]
2022 IEEE 19th International Symposium on Biomedical Imaging
(ISBI), 2022 In this paper, we propose a hybrid approach for multi-object microbial cell segmentation. The approach combines an ML-based detection with a geometry-aware variational-based segmentation using B-splines that are parametrized based on a geometric model of the cell shape. The detection is done first using YOLOv5.
arxiv +1 more source
Functional architecture and global properties of the Corynebacterium glutamicum regulatory network: novel insights from a dataset with a high genomic coverage [PDF]
Journal of Biotechnology 257:199-210 (2017), 2016 Corynebacterium glutamicum is a Gram-positive, anaerobic, rod-shaped soil bacterium able to grow on a diversity of carbon sources like sugars and organic acids. It is a biotechnological relevant organism because of its highly efficient ability to biosynthesize amino acids, such as L-glutamic acid and L-lysine. Here, we reconstructed the most complete C.
arxiv +1 more source
Quantitative modelling of nutrient-limited growth of bacterial colonies in microfluidic cultivation [PDF]
J. Royal Soc. Interface 15, 20170713 (2018), 2018 Nutrient gradients and limitations play a pivotal role in the life of all microbes, both in their natural habitat as well as in artificial, microfluidic systems. Spatial concentration gradients of nutrients in densely packed cell configurations may locally affect the bacterial growth leading to heterogeneous micropopulations.
arxiv +1 more source
Corynebacterium glutamicum regulation beyond transcription: Organizing principles and reconstruction of an extended regulatory network incorporating regulations mediated by small RNA and protein-protein interactions [PDF]
arXiv, 2021 Corynebacterium glutamicum is a Gram-positive bacterium found in soil where the condition changes demand plasticity of the regulatory machinery. The study of such machinery at the global scale has been challenged by the lack of data integration. Here, we report three regulatory network models for C.
arxiv
Gut Dysbiosis and Neurobehavioral Alterations in Rats Exposed to Silver Nanoparticles [PDF]
Scientific Reports 7, Article number: 2822 (2017), 2017 Due to their antimicrobial properties, silver nanoparticles (AgNPs) are being used in non-edible and edible consumer products. It is not clear though if exposure to these chemicals can exert toxic effects on the host and gut microbiome. Conflicting studies have been reported on whether AgNPs result in gut dysbiosis and other changes within the host. We
arxiv +1 more source
Multivariate cluster point process to quantify and explore multi-entity configurations: Application to biofilm image data [PDF]
arXiv, 2022 Clusters of similar or dissimilar objects are encountered in many fields. Frequently used approaches treat the central object of each cluster as latent. Yet, often objects of one or more types cluster around objects of another type. Such arrangements are common in biomedical images of cells, in which nearby cell types likely interact.
arxiv
Patchoulol Production with Metabolically Engineered Corynebacterium glutamicum [PDF]
Genes, 2018 Patchoulol is a sesquiterpene alcohol and an important natural product for the perfume industry. Corynebacterium glutamicum is the prominent host for the fermentative production of amino acids with an average annual production volume of ~6 million tons. Due to its robustness and well established large-scale fermentation, C.
Henke, Nadja Alina +8 more
openaire +3 more sources
Lysine uptake and exchange in Corynebacterium glutamicum
Journal of Bacteriology, 1990 Resting cells of Corynebacterium glutamicum (ATCC 13032) accumulate [14C]lysine by a transport system with a relatively high affinity (10 microMs) and a low maximum velocity (0.15 nmol/min per mg [dry weight]). Uptake of lysine was not inhibited by uncouplers or by ionophores affecting the ion gradients and the energetic state of the cell.
Reinhard Krämer, Stefan Bröer
openaire +4 more sources
Biosynthesis of Chondroitin in Engineered Corynebacterium glutamicum
Journal of Microbiology and Biotechnology, 2019 Chondroitin, the precursor of chondroitin sulfate, which is an important polysaccharide, has drawn significant attention due to its applications in many fields. In the present study, a heterologous biosynthesis pathway of chondroitin was designed in a GRAS (generally recognized as safe) strain C. glutamicum.
Huimin Yu +3 more
openaire +4 more sources
Formation of volutin granules inCorynebacterium glutamicum [PDF]
FEMS Microbiology Letters, 2005 Volutin granules are intracellular storages of complexed inorganic polyphosphate (poly P). Histochemical staining procedures differentiate between pathogenic corynebacteria such as Corynebacterum diphtheriae (containing volutin) and non-pathogenic species, such as C. glutamicum. Here we report that strains ATCC13032 and MH20-22B of the non-pathogenic C.
Pallerla, S. R. +6 more
openaire +3 more sources