Results 1 to 10 of about 10,021 (182)

Design and validation of an inducible and curable EvolvR system for directed evolution in Corynebacterium glutamicum [PDF]

Frontiers in Bioengineering and Biotechnology
The EvolvR system was developed as a tool for the continuous diversification of user-defined genomic loci in Escherichia coli. To make EvolvR accessible for enzyme engineering in Corynebacterium glutamicum, this work focused on the design and ...
Paul M. Will, Vanessa L. Göttl, Jan Seeger, Volker F. Wendisch, Nadja A. Henke   +4 more
doaj   +2 more sources

Synthetic biology tools for engineering Corynebacterium glutamicum

Computational and Structural Biotechnology Journal, 2023
Corynebacterium glutamicum is a promising organism for the industrial production of amino acids, fuels, and various value-added chemicals. From the whole genome sequence release, C. glutamicum has been valuable in the field of industrial microbiology and
Jina Yang, Geunyung Park, Sang Woo Seo
exaly   +3 more sources

Systems metabolic engineering of Corynebacterium glutamicum for efficient l-tryptophan production [PDF]

Synthetic and Systems Biotechnology
Corynebacterium glutamicum is a versatile industrial microorganism for producing various amino acids. However, there have been no reports of well-defined C. glutamicum strains capable of hyperproducing l-tryptophan.
Yufei Dong, Zhen Chen
doaj   +2 more sources

CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l-proline exporter for l-proline hyperproduction

Nature Communications, 2022
Corynebacterium glutamicum is a major workhorse in industrial biomanufacturing of amino acids. Here, the authors employ CRISPR-assisted rational flux-tuning and CRISPRi screening of a L-proline exporter to covert a wild-type C.
Jiao Liu, Moshi Liu, Tuo Shi, Guannan Sun, Ning Gao, Xiaojia Zhao, Xuan Guo, Xiaomeng Ni, Qianqian Yuan, Jinhui Feng, Zhemin Liu, Yanmei Guo, Jiuzhou Chen, Yu Wang, Ping Zheng, Jibin Sun   +15 more
doaj   +1 more source

Iron Delivery through Membrane Vesicles in Corynebacterium glutamicum

Microbiology Spectrum, 2023
Bacterial cells form and release membrane vesicles (MVs) originating from cellular membranes. In recent years, many biological functions of bacterial MVs have been identified.
Kayuki Kawashima, Toshiki Nagakubo, Nobuhiko Nomura, Masanori Toyofuku   +3 more
doaj   +1 more source

Advances and Perspectives for Genome Editing Tools of Corynebacterium glutamicum

Frontiers in Microbiology, 2021
Corynebacterium glutamicum has been considered a promising synthetic biological platform for biomanufacturing and bioremediation. However, there are still some challenges in genetic manipulation of C. glutamicum.
Qingzhuo Wang, Jiao Zhang, Jiao Zhang, Naief H. Al Makishah, Xiaoman Sun, Zhiqiang Wen, Zhiqiang Wen, Yu Jiang, Sheng Yang, Sheng Yang   +9 more
doaj   +1 more source

Application of Corynebacterium glutamicum engineering display system in three generations of biorefinery

Microbial Cell Factories, 2022
The fermentation production of platform chemicals in biorefineries is a sustainable alternative to the current petroleum refining process. The natural advantages of Corynebacterium glutamicum in carbon metabolism have led to C. glutamicum being used as a
Kerui Lin, Shuangyan Han, Suiping Zheng
doaj   +1 more source

Blue-white colony screening on acetate and glucose in Corynebacterim glutamicum

浙江大学学报. 农业与生命科学版, 2002
To develop the blue-white colony screening with promoter test vector in Corynebacterium glutamicum, two vectors pROH7 and pROHO were constructed and transformed into Corynebacterium glutamicum ATCC 14752.
RUAN Hong, Robert Gerstmeir, Bernhard Eikmanns   +2 more
doaj   +1 more source

Production of l-glutamate family amino acids in Corynebacterium glutamicum: Physiological mechanism, genetic modulation, and prospects

Synthetic and Systems Biotechnology, 2021
l-glutamate family amino acids (GFAAs), consisting of l-glutamate, l-arginine, l-citrulline, l-ornithine, l-proline, l-hydroxyproline, γ-aminobutyric acid, and 5-aminolevulinic acid, are widely applied in the food, pharmaceutical, cosmetic, and animal ...
Qi Sheng, Xiao-Yu Wu, Xinyi Xu, Xiaoming Tan, Zhimin Li, Bin Zhang   +5 more
doaj   +1 more source

AftD functions as an α1 → 5 arabinofuranosyltransferase involved in the biosynthesis of the mycobacterial cell wall core

The Cell Surface, 2018
Arabinogalactan (AG) is an essential structural macromolecule present in the cell wall of Mycobacterium tuberculosis, serving to connect peptidoglycan with the outer mycolic acid layer.
Luke J. Alderwick, Helen L. Birch, Karin Krumbach, Michael Bott, Lothar Eggeling, Gurdyal S. Besra   +5 more
doaj   +1 more source