Results 31 to 40 of about 1,990 (174)

Continuous Purification of mRNA Produced by In Vitro Transcription Using High Performance Countercurrent Membrane Purification

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Messenger ribonucleic acid (mRNA) therapeutics produced by in vitro transcription must be purified to remove residual enzymes and free nucleotides. This study examines the use of high‐performance countercurrent membrane purification (HPCMP) for the purification of mRNA therapeutics based on differences in the rate of diffusion across ...
Ziqiao Wang, Amin Javidanbardan, Ali Behboudi, Andrew L. Zydney   +3 more
wiley   +1 more source

Application of commercial ultrafiltration membranes for protein purification by high performance countercurrent membrane purification (HPCMP)

Biotechnology Progress, EarlyView.
Abstract High performance countercurrent membrane purification (HPCMP) has emerged as a promising diffusive separation strategy for the purification of recombinant proteins and monoclonal antibodies (mAbs). However, prior studies employed dialysis membranes, which are designed for diffusive separations but have not been validated for biopharmaceutical ...
Aylin Mohammadzadehmarandi, Nate Heller, Mohammadreza Ahmadimashhadi, Jon Petrone, Nicholas Marchand, Siavash Darvishmanesh, Ka Zhang, Stefano Menegatti, Andrew L. Zydney   +8 more
wiley   +1 more source

Advances in Anthocyanin Recovery and Purification From Natural Sources: Bioavailability, Mechanistic Action in the Human Body and Applications in Novel Food Development

Food Chemistry International, EarlyView.
This review highlights green recovery, purification, stabilisation, bioavailability, health relevance, and novel food applications of anthocyanins from natural and agro‐industrial sources. ABSTRACT Anthocyanins are water‐soluble natural pigments and multifunctional food ingredients that support clean‐label colour, antioxidant activity, and value‐added ...
Chandan Kumar Sahu, Soumitra Banerjee, Venkata Krishna Bayineni, Ravi‐Kumar Kadeppagari, Amit Arora   +4 more
wiley   +1 more source

Isolation and identification of highly active anticholinesterase ingredients from fermented soybean products

CyTA - Journal of Food, 2017
Inhibitory activities of ethanol extracts from seven fermented soybean products, including douchi, sufu, stinky tofu, to acetylcholinesterase were determined, and crude extract from Yongchuan douchi, which showed the strongest inhibitory effect, was ...
Yunbin Zhang, Mengyue Wei, Weidi Li, Yifei Wang, Jingwen Wang   +4 more
doaj   +1 more source

Toward Robust Optimal Control of Chromatographic Separation Processes With Controlled Flow Reversal

Proceedings in Applied Mathematics and Mechanics, Volume 26, Issue 3, September 2026.
ABSTRACT Column liquid chromatography is an important technique applied in the production of biopharmaceuticals, specifically for the separation of biological macromolecules such as proteins. When setting up process conditions, it is crucial that the purity of the product is sufficiently high, even in the presence of perturbations in the process ...
Dominik H. Cebulla, Christian Kirches, Andreas Potschka   +2 more
wiley   +1 more source

The kidney antifibrotic effects of 5,7,3′,4′,5′-pentamethoxyflavone from Bauhinia championii in streptozotocin-induced diabetic rats: in vivo and in vitro experiments

Pharmaceutical Biology, 2023
Context The antidiabetic effects of flavonoids have been reported, but it is still unclear whether 5,7,3′,4′,5′-pentamethoxyflavone, isolated from Bauhinia championii Benth.
Jialin Li, Xiaocui Huang, Kailun He, Suzhen Wu   +3 more
doaj   +1 more source

Understanding Mass Spectrometry: From Ion Generation to Spectral Interpretation

Journal of Mass Spectrometry, Volume 61, Issue 8, August 2026.
ABSTRACT This tutorial provides a structured introduction to mass spectrometry (MS), with particular emphasis on electrospray ionization (ESI)–based measurements. It is intended to support researchers from chemistry and related disciplines in developing a coherent understanding of MS principles, instrumentation, and data interpretation.
Arnold Steckel, Dávid Papp, Gitta Schlosser   +2 more
wiley   +1 more source

Sex‐Specific Regulation of Glycemic Homeostasis by Theabrownin from Pu‐erh Tea

Advanced Science, Volume 13, Issue 38, 9 July 2026.
Pu‐erh tea's key component, theabrownin (TB), lowers blood glucose in a sex‐specific manner. In females, estrogen boosts intestinal MUC2 production, which dramatically enhances TB's ability to inhibit the carbohydrate‐digesting enzyme α‐glucosidase.
Yang Li, Junliang Kuang, Dan Zheng, Jieyi Wang, Kun Ge, Chun Qu, Xiaojiao Zheng, Zhenxing Ren, Fengjie Huang, Mingliang Zhao, Yimin Sun, Huiheng Zhang, Keke Ding, Xixi Xia, Yajun Tang, Lu Liu, Jian Zhou, Fei Gao, Xiaohui Ma, Yongquan Xu, Guoxiang Xie, Aihua Zhao, Wei Jia   +22 more
wiley   +1 more source

Isolation of phenolic compounds from Rheedia longifolia by high-speed countercurrent chromatography

Rodriguésia
Rheedia longifolia is a species of the Clusiaceae family, popularly known as bacupari. Pharmacological studies conducted by our group revealed that the raw methanolic extract of its leaves, along with its fractions, induces both inflammatory and ...
Ana Luiza Rangel Bérenger, Flávia da Cunha Camillo, Lavínia de Carvalho Brito, Maria Auxiliadora Coelho Kaplan, Maria Raquel Figueiredo   +4 more
doaj   +1 more source

Isolation of Phenolics from by Combined Counter-current Chromatography and Gel-Filtration

Natural Product Communications, 2014
Nine phenolic compounds, quercetin, epi -catechin, catechin, 4-hydroxybenzoic acid, kaempferol 3- O -β-glucopyranoside, quercetin 3- O -β-glucopyranoside, quercetin 3- O -6″- trans -coumaroyl-β-glucoside, kaempferol 3- O -β-rutinoside and quercetin 3- O -
Fernanda das Neves Costa, Marcos Daniel da Silva, Ricardo Moreira Borges, Gilda Guimarães Leitão   +3 more
doaj   +1 more source