Results 71 to 80 of about 166,609 (297)
Selection of antibody-binding covalent aptamers
Communications ChemistryAptamers are oligonucleotides with antibody-like binding function, selected from large combinatorial libraries. In this study, we modified a DNA aptamer library with N-hydroxysuccinimide esters, enabling covalent conjugation with cognate proteins.
Noah Soxpollard +3 more
doaj +1 more source
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Postsynthetic Covalent Modification of a Neutral Metal−Organic Framework
, 2016 The covalent modification of the metal−organic framework IRMOF-3 has been achieved using acetic anhydride. Mass spectrometry and 1H NMR evidence shows that the free amine groups in IRMOF-3 are converted to amides in high yields. Control experiments and X-
Zhenqiang Wang (1459963) +1 more
core +2 more sources
Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9
mBio, 2015 The genomic DNAs of tailed bacteriophages are commonly modified by the attachment of chemical groups. Some forms of DNA modification are known to protect phage DNA from cleavage by restriction enzymes, but others are of unknown function.
Alexandra L. Bryson +7 more
doaj +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Deciphering the phosphorylation of chitosan through complementary 1H and 31P{1H} DOSY NMR
Carbohydrate Polymer Technologies and ApplicationsChemical modification of chitosan through phosphorylation has gained significant attention for expanding its applications. However, confirming whether phosphorylating agents form covalent bonds with the chitosan backbone or remain as non-covalently ...
Souhaib Abouricha +8 more
doaj +1 more source
, 2019 Brd4, a member of the bromodomain and extraterminal domain (BET) family, has emerged as a promising epigenetic target in cancer and inflammatory disorders.
Michael Olp (6944188) +5 more
core +1 more source
Selective Covalent Targeting of SARS-CoV-2 Main Protease by Enantiopure Chlorofluoroacetamide
, 2021 The pandemic of COVID-2019 has urged the development of antiviral agents against its causative pathogen SARS-CoV-2. The main protease (Mpro), a cysteine protease essential for viral replication, is a promising protein target.
Rui, Hamada +9 more
core +1 more source
Rho GTPase regulation by miRNAs and covalent modifications [PDF]
Trends in Cell Biology, 2012 To date, most studies of Rho GTPase regulation have focused on the classic GTPase cycle - GTP binding and hydrolysis - controlled by guanine nucleotide exchange factors (GEFs), GTPase-activating proteins (GAPs) and GDP-dissociation inhibitors (GDIs).
Ming, Liu +3 more
openaire +2 more sources