Results 51 to 60 of about 1,431 (214)

Covalent Modification Cycles through the Spatial Prism [PDF]

Biophysical Journal, 2013
Covalent modification cycles are basic units and building blocks of posttranslational modification and cellular signal transduction. We systematically explore different spatial aspects of signal transduction in covalent modification cycles by starting with a basic temporal cycle as a reference and focusing on steady-state signal transduction.
Alam-Nazki, Aiman, Krishnan, J.
openaire   +2 more sources

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Selection of antibody-binding covalent aptamers

Communications Chemistry
Aptamers are oligonucleotides with antibody-like binding function, selected from large combinatorial libraries. In this study, we modified a DNA aptamer library with N-hydroxysuccinimide esters, enabling covalent conjugation with cognate proteins.
Noah Soxpollard, Sebastian Strauss, Ralf Jungmann, Iain S. MacPherson   +3 more
doaj   +1 more source

Recent progress on thermal conductivity of graphene filled epoxy composites

Nano Materials Science, 2022
With the rapid development of the electronic industry, the requirements for packaging materials with high thermal conductivity (TC) are getting higher and higher. Epoxy is widely used as package material for electronic package applications.
Ruicong Lv, Yanjuan Ren, Haichang Guo, Shulin Bai   +3 more
doaj   +1 more source

Covalent modification of nitrogenase MoFe protein by ADP [PDF]

Biochemical Journal, 1997
MgADP- reacted with the nitrogenase molybdenum–iron (MoFe) protein of Klebsiella pneumoniae (Kp1) over a period of 2 h to yield a stable, catalytically active conjugate. The isolated protein exhibited a new, broad 31P NMR resonance at -1 p.p.m. lacking phosphorus J coupling. The adenine ring of [8-14C]ADP remained associated with the conjugate.
R W, Miller, R R, Eady, C, Gormal, S A, Fairhurst, B E, Smith   +4 more
openaire   +2 more sources

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9

mBio, 2015
The genomic DNAs of tailed bacteriophages are commonly modified by the attachment of chemical groups. Some forms of DNA modification are known to protect phage DNA from cleavage by restriction enzymes, but others are of unknown function.
Alexandra L. Bryson, Young Hwang, Scott Sherrill-Mix, Gary D. Wu, James D. Lewis, Lindsay Black, Tyson A. Clark, Frederic D. Bushman   +7 more
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Deciphering the phosphorylation of chitosan through complementary 1H and 31P{1H} DOSY NMR

Carbohydrate Polymer Technologies and Applications
Chemical modification of chitosan through phosphorylation has gained significant attention for expanding its applications. However, confirming whether phosphorylating agents form covalent bonds with the chitosan backbone or remain as non-covalently ...
Souhaib Abouricha, Cédric Totee, Steven Le Vot, Abdelouahd Oukhrib, Ouassim Ghodbane, Mohammed Lahcini, Frédéric Favier, Bruno Alonso, Hicham Ben Youcef   +8 more
doaj   +1 more source