Results 31 to 40 of about 1,431 (214)

Characterization of Whey Protein Isolate Covalently Modified with Phenolic Compounds 2: Physiochemical and Emulsifying Properties [PDF]

Journal of Food and Dairy Sciences, 2019
This work was performed to study the effect of the covalent modifications of whey protein isolate (WP) by chlorogenic acid (CA) and rosmarinic acid (RA) on their physiochemical and emulsifying properties.
Salwa Arafa, M. Ali
doaj   +1 more source

A novel covalent modification of nitrogenase in a cyanobacterium

FEBS Letters, 2000
In extracts of the unicellular cyanobacterium Gloeothece, the Fe‐protein of nitrogenase can be separated by SDS–PAGE into two antigenically identifiable components. Unlike the situation in photosynthetic bacteria such as Rhodospirillum rubrum, these two forms do not arise from covalent modification of the protein by ADP‐ribosylation.
Gallon, John R., Cheng, Jiujun, Dougherty, Lisa J., Gallon, Victoria A., Hilz, Helmuth, Pederson, Dennis M., Richards, Helen M., Rüggeberg, Sabrina, Smith, Christopher J.   +8 more
openaire   +2 more sources

Covalent modification of a glutamic acid inspired by HaloTag technology

Nature Communications
For targeted covalent protein modification at low-reactivity aspartates and glutamates, new methods are in high demand. We report a technique inspired by the HaloTag technology, which employs nucleophilic substitution at chloroalkane-functionalised ...
Ruirui Zhang, Jie Liu, Raphael Gasper, Anke Unger, Farnusch Kaschani, Markus Kaiser, Petra Janning, Herbert Waldmann   +7 more
doaj   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Graphene oxide grafted with polydimethylsiloxane modified polyurethane

Nano Select, 2021
In order to improve the dispersion of graphene oxide (GO) in polymers, we synthesized polyurethane (PU) chains with long polydimethylsiloxane (PDMS) chains as the “soft segment” and hexamethylene diisocyanate (HDI) as the “hard segment”, then the PU ...
Danian Liu, Jingwen Wang, Xinhua Gao, Houqing Wang, Hua Ren   +4 more
doaj   +1 more source

Assessing reversible and irreversible binding effects of kinase covalent inhibitors through ADP-Glo assays

STAR Protocols, 2021
Summary: Typical enzymatic inhibition assays often demonstrate improved potency for kinase covalent inhibitors compared to reversible inhibitors. This can primarily be attributed to the irreversible mode of action and could affect the evaluations of the ...
Martin Schröder, Apirat Chaikuad
doaj   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Protein covalent modification by biologically active quinones [PDF]

Journal of the Serbian Chemical Society, 2004
The avarone/avarol quinone/hydroquinone couple shows considerable antitumor activity. In this work, covalent modification of b-lactoglobulin by avarone and its derivatives as well as by the synthetic steroidal quinone 2,5(10)-estradiene-1,4,17-trione and
MIROSLAV J. GASIC, BOGDAN SOLAJA, DRAGANA MILIC, TATJANA BOZIC, NATASA BOZIC, ZORAN VUJCIC, IRENA NOVAKOVIC, DUSAN SLADIC   +7 more
doaj  

Hydrogenated Germanene Nanosheets as an Antioxidative Defense Agent for Acute Kidney Injury Treatment

Advanced Science, 2022
Acute kidney injury (AKI) is a sudden kidney dysfunction caused by aberrant reactive oxygen species (ROS) metabolism that results in high clinical mortality. The rapid development of ROS scavengers provides new opportunities for AKI treatment.
Zhixin Chen, Fenggang Qi, Wujie Qiu, Chenyao Wu, Ming Zong, Min Ge, Deliang Xu, Yanling You, Ya‐Xuan Zhu, Zhimin Zhang, Han Lin, Jianlin Shi   +11 more
doaj   +1 more source

Methodology of stable peptide based on propargylated sulfonium

Biochemistry and Biophysics Reports, 2023
Peptides can be used as effective molecular tool for covalent modification of proteins and play important roles in ligand directed covalent modification. Tyr-selective protein modifications exert a profound impact on protein functionality.
Heng Li, Zhanfeng Hou, Yuena Wang, Ziyuan Zhou, Jin Cai, Qilei Xin, Feng Yin, Zigang Li, Naihan Xu   +8 more
doaj   +1 more source