Results 91 to 100 of about 2,379,457 (382)

Cell‐cycle‐specific lesion evolution rather than inhibition of double‐strand‐break repair underpins cisplatin radiosensitization

Molecular Oncology, EarlyView.
We analyze cisplatin–DNA adducts (CDAs) and double‐strand breaks (DSBs) in a cell‐cycle‐dependent manner. We find that CDAs form similarly across all cell cycle phases. DSBs arise only in S‐phase. CDAs might not directly impair DSB repair, but S‐phase DSB lesions evolve in the presence of CDAs and disrupt repair in G2, also causing radiosensitization ...
Ye Qiu, Xixi Lin, Emil Mladenov, Veronika Mladenova, Jürgen Thomale, Ali Sak, Yao Wang, Yunxuan Deng, Eleni Gkika, Martin Stuschke, George Iliakis   +10 more
wiley   +1 more source

RanBP2-Mediated SUMOylation Promotes Human DNA Polymerase Lambda Nuclear Localization and DNA Repair [PDF]

, 2020
Cellular DNA is under constant attack by a wide variety of agents, both endogenous and exogenous. To counteract DNA damage, human cells have a large collection of DNA repair factors. Among them, DNA polymerase lambda (Polλ) stands out for its versatility,
Cortés Ledesma, Felipe, García Domínguez, Mario, Herrero Ruiz, Andrés M., Moreno Oñate, Marta, Ruiz Pérez, José Francisco   +4 more
core   +1 more source

Quantum Chemical Methods for Modeling Covalent Modification of Biological Thiols

Journal of Computational Chemistry, 2019
Targeted covalent inhibitor drugs require computational methods that go beyond simple molecular‐mechanical force fields in order to model the chemical reactions that occur when they bind to their targets.
Ernest Awoonor-Williams, W. Isley, Stephen G. Dale, E. Johnson, Haibo Yu, A. Becke, B. Roux, C. Rowley   +7 more
semanticscholar   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

The anticancer natural product ophiobolin A induces cytotoxicity by covalent modification of phosphatidylethanolamine

eLife, 2016
Phenotypic screens allow the identification of small molecules with promising anticancer activity, but the difficulty in characterizing the mechanism of action of these compounds in human cells often undermines their value as drug leads.
Christopher Chidley, Sunia A Trauger, Kıvanç Birsoy, Erin K O'Shea   +3 more
doaj   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

Selection of antibody-binding covalent aptamers

Communications Chemistry
Aptamers are oligonucleotides with antibody-like binding function, selected from large combinatorial libraries. In this study, we modified a DNA aptamer library with N-hydroxysuccinimide esters, enabling covalent conjugation with cognate proteins.
Noah Soxpollard, Sebastian Strauss, Ralf Jungmann, Iain S. MacPherson   +3 more
doaj   +1 more source

Uncovering the covalent inhibitors of SARS-CoV-2 Mpro in Tibetan edible herb Rhodiola crenulata and their synergistic anti-Mpro mechanism

Journal of Pharmaceutical Analysis
The main protease (Mpro) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been validated as a therapeutic target for antiviral drug development, given its critical role in the viral life cycle.
Guang-Hao Zhu, Ya-Ni Zhang, Yuan Xiong, Xu-Dong Hou, Qing-Guang Zhang, Zhao-Qin Zhang, Xiao-Yu Zhuang, Wei-Dong Zhang, Guang-Bo Ge   +8 more
doaj   +1 more source

On the origin of the C induced $p4g$ reconstruction of Ni(001)

, 2005
First principles calculations of the geometric and electronic structures have been performed for two coverages (0.25 ML and 0.5 ML) of C on Ni(001) to understand the mechanism of the Ni(001) reconstruction induced by carbon adsorption.
Abdelkader Kara, C. Q. Sun, D. Singh, D. Vanderbilt, P. Blaha, Sampyo Hong, Sergey Stolbov, Talat S. Rahman, Y. Gauther   +8 more
core   +1 more source

Multistep, sequential control of the trafficking and function of the multiple sulfatase deficiency gene product, SUMF1 by PDI, ERGIC-53 and ERp44. [PDF]

, 2008
Sulfatase modifying factor 1 (SUMF1) encodes for the formylglicine generating enzyme, which activates sulfatases by modifying a key cysteine residue within their catalytic domains. SUMF1 is mutated in patients affected by multiple sulfatase deficiency, a
Annunziata F., BALLABIO, ANDREA, Cosma M. P., Cozzolino M., FRALDI, ALESSANDRO, Lombardi A., MONTI, MARIA, PUCCI, PIETRO, Sitia R., Spampanato C., Zito E.   +10 more
core   +1 more source