Results 141 to 150 of about 4,339 (163)
Some of the next articles are maybe not open access.
Analytica Chimica Acta, 2009
The introduction of genetically modified (GM) crops into the market has raised a general alertness relating to the control and safety of foods. The applicability of protein separation hyphenated to mass spectrometry to identify the bacterial enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) protein expressed in GM crops has been previously reported
Mireia Fernández, Ocaña +4 more
openaire +2 more sources
The introduction of genetically modified (GM) crops into the market has raised a general alertness relating to the control and safety of foods. The applicability of protein separation hyphenated to mass spectrometry to identify the bacterial enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) protein expressed in GM crops has been previously reported
Mireia Fernández, Ocaña +4 more
openaire +2 more sources
Development and Characterization of a CP4 EPSPS‐Based, Glyphosate‐Tolerant Corn Event
Crop Science, 20055‐Enol‐pyruvylshikimate‐3‐phosphate synthase from Agrobacterium sp. CP4 (CP4 EPSPS) confers tolerance to the nonselective herbicide glyphosate (marketed under the trade name Roundup) when sufficiently expressed in transgenic plants. Dual CP4 EPSPS transgene cassettes were transformed into corn (Zea mays L.) under the transcriptional regulatory control ...
G. R. Heck +25 more
exaly +2 more sources
Journal of Mass Spectrometry, 2019
AbstractSample preparation methods used for genetically modified organisms (GMOs) analysis are often time consuming, require extensive manual manipulation, and result in limited amounts of purified protein, which may complicate the detection of low‐abundance GM protein.
Shobha Devi +3 more
openaire +2 more sources
AbstractSample preparation methods used for genetically modified organisms (GMOs) analysis are often time consuming, require extensive manual manipulation, and result in limited amounts of purified protein, which may complicate the detection of low‐abundance GM protein.
Shobha Devi +3 more
openaire +2 more sources
Food Chemistry, 2021
A simple electrochemical immunosensor based on nitrogen-doped graphene and polyamide-amine (GN-PAM) composites was proposed for the detection of the CP4-EPSPS protein in genetically modified (GM) crops. In this immunosensor, the amplification of the detection signal was realized through antibodies labeled with gold nanoparticles (AuNPs).
Haijuan, Zeng +7 more
openaire +2 more sources
A simple electrochemical immunosensor based on nitrogen-doped graphene and polyamide-amine (GN-PAM) composites was proposed for the detection of the CP4-EPSPS protein in genetically modified (GM) crops. In this immunosensor, the amplification of the detection signal was realized through antibodies labeled with gold nanoparticles (AuNPs).
Haijuan, Zeng +7 more
openaire +2 more sources
Selection of DNA Aptamers for Detection of CP4 EPSPS Proteinin GM Soybean
2018Thai Agricultural Research Journal, 36, 2, 141 ...
Adcharapun Chaicharoen +1 more
openaire +1 more source
Transgenic potato lines expressing CP4-EPSP synthase exhibit resistance against glyphosate
Plant Cell, Tissue and Organ Culture (PCTOC), 2019Potato crops are particularly vulnerable to weed competition from emergence to canopy closure and are subject to significant yield loss. Glyphosate is broad spectrum herbicide used to control weeds worldwide. In order to incorporate glyphosate resistant trait in four potato cultivars (Lady Olympia, Desiree, Agria and Granola), an efficient, cost ...
Allah Bakhsh +4 more
openaire +2 more sources
[Preparation and characterization of monoclonal antibody against CP4-EPSPS].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2011To prepare monoclonal antibody (mAb) against CP4-EPSPS, which could be applied to the development of gold colloidal rapid diagnostic kit for the specific detection of GMO.BALB/c mice were immunized with CP4-EPSPS. Splenocytes of the immunized mice were collected and fused with the mouse myeloma cell line Sp2/0 cells.
Ling-xia, Jing +7 more
openaire +1 more source
Foodborne Pathogens and Disease
Protein-based detection methods, enzyme-linked immunosorbent assays (ELISAs) and lateral flow strips, have been widely used for rapid, specific, and sensitive detection of genetically modified organisms (GMOs). However, the traditional ELISA method for the quantitative detection of GMOs has certain limitations.
Jiong Li
exaly +3 more sources
Protein-based detection methods, enzyme-linked immunosorbent assays (ELISAs) and lateral flow strips, have been widely used for rapid, specific, and sensitive detection of genetically modified organisms (GMOs). However, the traditional ELISA method for the quantitative detection of GMOs has certain limitations.
Jiong Li
exaly +3 more sources
Plant Cell Reports, 2014
Highly tolerant herbicide-resistant transgenic rice was developed by expressing codon-modified synthetic CP4--EPSPS. The transformants could tolerate up to 1% commercial glyphosate and has the potential to be used for DSR (direct-seeded rice). Weed infestation is one of the major biotic stress factors that is responsible for yield loss in direct-seeded
Sushil, Chhapekar +8 more
openaire +2 more sources
Highly tolerant herbicide-resistant transgenic rice was developed by expressing codon-modified synthetic CP4--EPSPS. The transformants could tolerate up to 1% commercial glyphosate and has the potential to be used for DSR (direct-seeded rice). Weed infestation is one of the major biotic stress factors that is responsible for yield loss in direct-seeded
Sushil, Chhapekar +8 more
openaire +2 more sources
Monoclonal antibody production for CP4 EPSPS detection assays
Environmental Biology Research, 2021A-Mi Yoon, Il Ryong Kim, Wonkyun Choi
openaire +1 more source