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Talanta, 2022 Soybean is the most produced crop in Argentina, and 99 % corresponds to genetically modified soybean. One of the main produced varieties is Roundup Ready® soybean (RR), which was modified to express the enzyme CP4 5-enolpyruvylshikimate 3-phosphate synthase (CP4 EPSPS), which confers resistance to glyphosate, the main herbicide worldwide used.
Patricia Gabriela Molina +2 more
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Food Chemistry, 2023
Two different models of electrochemiluminescence (ECL) immunosensors for the sensitive and quantitative detection of the CP4-EPSPS protein in genetically modified (GM) crops were proposed in this study. One was a signal-reduced ECL immunosensor based on nitrogen-doped graphene, graphitic carbon nitride and polyamide-amine (GN-PAMAM-g-C3N4) composites ...
Jinbin Wang, Lemei Zhu, Haijuan Zeng
exaly +3 more sources
Two different models of electrochemiluminescence (ECL) immunosensors for the sensitive and quantitative detection of the CP4-EPSPS protein in genetically modified (GM) crops were proposed in this study. One was a signal-reduced ECL immunosensor based on nitrogen-doped graphene, graphitic carbon nitride and polyamide-amine (GN-PAMAM-g-C3N4) composites ...
Jinbin Wang, Lemei Zhu, Haijuan Zeng
exaly +3 more sources
Foodborne Pathogens and Disease
Protein-based detection methods, enzyme-linked immunosorbent assays (ELISAs) and lateral flow strips, have been widely used for rapid, specific, and sensitive detection of genetically modified organisms (GMOs). However, the traditional ELISA method for the quantitative detection of GMOs has certain limitations.
Jiong Li
exaly +3 more sources
Protein-based detection methods, enzyme-linked immunosorbent assays (ELISAs) and lateral flow strips, have been widely used for rapid, specific, and sensitive detection of genetically modified organisms (GMOs). However, the traditional ELISA method for the quantitative detection of GMOs has certain limitations.
Jiong Li
exaly +3 more sources
Mass spectrometric detection of CP4 EPSPS in genetically modified soya and maize
Rapid Communications in Mass Spectrometry, 2007Abstract The potential of protein fractionation hyphenated to mass spectrometry (MS) to detect and characterize the transgenic protein present in Roundup Ready™ soya and maize has been investigated. Genetically modified (GM) soya and maize contain the 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS) gene from
Mireia Fernández, Ocaña +4 more
openaire +2 more sources
Development and Characterization of a CP4 EPSPS‐Based, Glyphosate‐Tolerant Corn Event
Crop Science, 20055‐Enol‐pyruvylshikimate‐3‐phosphate synthase from Agrobacterium sp. CP4 (CP4 EPSPS) confers tolerance to the nonselective herbicide glyphosate (marketed under the trade name Roundup) when sufficiently expressed in transgenic plants. Dual CP4 EPSPS transgene cassettes were transformed into corn (Zea mays L.) under the transcriptional regulatory control ...
G. R. Heck +25 more
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Gametic Selection by Glyphosate in Soybean Plants Hemizygous for the CP4 EPSPS Transgene
Crop Science, 2006Glyphosate application to glyphosate‐tolerant soybean [Glycine max (L.) Merr.] cultivars expressing a CP4 EPSPS transgene has become the most common method of soybean weed control in the USA. The objectives of this research were (i) to determine whether atypical segregation ratios for glyphosate tolerance and glyphosate sensitivity in the progeny of ...
David R. Walker +11 more
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Microchemical Journal, 2021
Abstract This work evaluates three generations of transgenic and non-transgenic soybean plants through multiomics namely ionomics and lipidomics. Transgenic variety was inserted with cp4-EPSPS gene, which confers resistance to glyphosate. Comparisons between soybeans were carried out from seeds obtained during summer season on south hemisphere, grown
Luana Ferreira da Costa +2 more
openaire +1 more source
Abstract This work evaluates three generations of transgenic and non-transgenic soybean plants through multiomics namely ionomics and lipidomics. Transgenic variety was inserted with cp4-EPSPS gene, which confers resistance to glyphosate. Comparisons between soybeans were carried out from seeds obtained during summer season on south hemisphere, grown
Luana Ferreira da Costa +2 more
openaire +1 more source
Analytica Chimica Acta, 2009
The introduction of genetically modified (GM) crops into the market has raised a general alertness relating to the control and safety of foods. The applicability of protein separation hyphenated to mass spectrometry to identify the bacterial enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) protein expressed in GM crops has been previously reported
Mireia Fernández, Ocaña +4 more
openaire +2 more sources
The introduction of genetically modified (GM) crops into the market has raised a general alertness relating to the control and safety of foods. The applicability of protein separation hyphenated to mass spectrometry to identify the bacterial enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) protein expressed in GM crops has been previously reported
Mireia Fernández, Ocaña +4 more
openaire +2 more sources